Volume 1, Issue 1 (1-2003)                   IJRM 2003, 1(1): 12-15 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Movassagh-Pour A A, Salehnia M, Pourfatollah A A, Moazzeni S M. The Effect of Murine Leukemia Inhibitory Factor on In Vitro Differentiation of Mouse Embryonic Stem Cells. IJRM. 2003; 1 (1) :12-15
URL: http://ijrm.ssu.ac.ir/article-1-1-en.html
Abstract:   (1859 Views)
Background: Embryonic stem cells (ESc) are pluripotent cells which have been used as a model to study the mechanism that control the embryogenesis and early mammalian development in vitro. The aim of this study was to isolate and produce embryonic stem cells from late blastocyst stage embryos in mice. Materials and Methods: Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 24 h in DMEM medium. 4-6 days after hatching, the inner cell masses (ICM) formed colonies which were then collected mechanically and trypsinized. Several subcultures were prepared in the medium supplemented with 0.1 mM 2 Mercaptoethanol, 1000 U/ml Leukemia Inhibitory Factor (LIF) and 10% Fetal Bovine Serum (FBS). The (ESc) were recognized by alkaline phosphates histochemistry using azo-coupling method. Results: The results demonstrated that a highly pluripotent stem cell line was derived from the blastocyst stage embryos of NMRI mice; however, the rate of colonies was as low as 10%. Conclusion: The LIF is effective to culture and maintain the isolated ICM colonies in undifferentiated condition in the absence of feeder layer.
Full-Text [PDF 170 kb]   (265 Downloads) |   |   Full-Text (HTML)  (129 Views)  
Type of Study: Original Article |

1. Anderson GB. (1992) Isolation and use of embryonic stem cells from livestock species. Anim Biotechnol 3: 165-175. [DOI:10.1080/10495399209525769]
2. Bongso A, Fong CY, Ng SC, Ratnam S. (1994) Isolation and culture of inner cell mass cells from human blastocysts. Hum Reprod 9: 2119-2117. [DOI:10.1093/oxfordjournals.humrep.a138401] [PMID]
3. Cole RJ, Edwards RC, Poul J. (1965) Cytodifferentiation in cell colonies and cell strains derived from cleaving ova and blastocysts of the rabbit. J Exp Cell Res 501-504. [DOI:10.1016/0014-4827(65)90201-6]
4. Doetschman T, Williams P, Maeda N. (1988) Establishment of hamster blastocyst derived embryonic stem cells. Dev Biol 127: 224-227. [DOI:10.1016/0012-1606(88)90204-7]
5. Donovan PJ, Stott D, Cairns LA, Heasman J, Wylie CC. (1986) Migratory and postmigratory mouse primordial germ cells behave differently in culture. Cell 44: 831-838. [DOI:10.1016/0092-8674(86)90005-X]
6. Evans MJ, Kaufman M. (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 242: 154-156. [DOI:10.1038/292154a0] [PMID]
7. Martin GR. (1981) Isolation of a pluripotent cell line from early mouse embryos cultured in medium contained with teratocarcinoma stem cells. Proc Nat Sci 78: 7634-7638. [DOI:10.1073/pnas.78.12.7634] [PMID] [PMCID]
8. Movassagh Pour A, Salehnia M, Pourfatollah M, Moazzeni SM. (2003) CFU-GM like colonies derived from embryonic stem cells cultured on the bone marrow stromal cells. Iranian Biomedical J (In press).
9. Odorico JS, Kaufman DS, Thomson JA. (2001) Multilineage differentiation from human embryonic stem cell lines. Stem Cells 19: 193-204. [DOI:10.1634/stemcells.19-3-193] [PMID]
10. Pera MF, Reubinoff BE, Truonson A. (2000) Human embryonic stem cells. Cell Science 113: 5-10.
11. Rathjeh PD, Lake J, Whyatt, Bettess MD, Rathjeh J. (1998) Properties and uses of embryonic stem cells: prospects for application to human biology and gene therapy. Reprod Fertil Dev 10: 31-47. [DOI:10.1071/R98041] [PMID]
12. Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A. (2000) Embryonic stem cell lines from human blastocyst: somatic differentiation in vitro. Nature Biotech. 18: 399-404. [DOI:10.1038/74447] [PMID]
13. Smith AG, Hooper L. (1987) Buffalo liver cell produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev Biol 121: 1-9. [DOI:10.1016/0012-1606(87)90132-1]
14. Smith AG, Heath JK, Donaldsmon DD, Wong GG, Moreau J, Stahl M, Rogers D. (1988) Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides. Nature 336: 688-690. [DOI:10.1038/336688a0] [PMID]
15. Smith AG. (1998) Cell therapy: In search of pluripotency. Current Biol 8; 802-804. [DOI:10.1016/S0960-9822(07)00504-0]
16. Smith AG. (1992) Mouse embryo stem cells: their identification, propagation and manipulation. Seminar in Cell Biol 3: 385-399. [DOI:10.1016/1043-4682(92)90010-S]
17. Thomson JA, Marshal VS. (1998) Primate embryonic stem cells. Curr Topics Dev Biol 38: 133-165. [DOI:10.1016/S0070-2153(08)60246-X]
18. Thomson JA, Kalishman J, Golos TS, Durning M, Harris CP, Becker RA, Hearn JP. (1995) Isolation of a primate embryonic stem cell line. Proc Natl Acad Sci USA 92: 7844-48. [DOI:10.1073/pnas.92.17.7844] [PMID] [PMCID]

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb