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Volume 16, Issue 11 (November 2018)
IJRM 2018, 16(11): 711-718 |
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Hosseini A, Zare S, Borzouei Z, Ghaderi Pakdel F. Cyclophosphamide-induced testicular toxicity ameliorate by American ginseng treatment: An experimental study. IJRM 2018; 16 (11) :711-718
URL: http://ijrm.ir/article-1-1300-en.html
URL: http://ijrm.ir/article-1-1300-en.html
1- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 2. Department of Biology, Faculty of Science, University of Urmia, Urmia, Iran
2- Department of Biology, Faculty of Science, University of Urmia, Urmia, Iran
3- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4- Solid Tumor Research Center Urmia. University of Medical Sciences, Urmia, Iran , info@fgpakdel.com
2- Department of Biology, Faculty of Science, University of Urmia, Urmia, Iran
3- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4- Solid Tumor Research Center Urmia. University of Medical Sciences, Urmia, Iran , info@fgpakdel.com
Abstract: (2702 Views)
Background: Cyclophosphamide (CP) is one of the most invasive chemotherapeutic agents, which used commonly despite of its wide spectrum toxicity. Clinical evidence showed toxic side effects of CP in multiple organ systems.
Objective: The objective of the present study was to evaluate the effects of American ginseng on CP-induced testicular toxicity in rats.
Materials and Methods: Adult male Wistar rats (220±30 gr) were randomly divided into four groups (n=7 in each). Group 1 as control received normal saline by gavage, group 2 received CP (6.1 mg/kg/day, i.p.) for a period of 50 days. Group 3 received American ginseng (500 mg/kg/day) by gavage. Group 4 received American ginseng (500 mg/kg/day) 1h prior to the administration of CP in the equal dose of group 2. The animals scarified one day after the last treatment and the effects of American ginseng on the sperm vital parameters, testicular functions, biochemical factors, and structural malformations evaluated.
Results: Serum testosterone concentration was significantly decreased whereas the level of malondialdehyde and DNA damage were significantly increased in animals of CP group (p<0.01). Co-administration of American ginseng reversed these parameters and improved recovery in CP+ginseng group. In addition, seminiferous tubules of testis severely damaged in the CP group but ginseng improved histologic changes in CP+ginseng group.
Conclusion: The findings confirmed the protective effects of American ginseng on toxicity induced by CP in the reproductive system of male rats.
Objective: The objective of the present study was to evaluate the effects of American ginseng on CP-induced testicular toxicity in rats.
Materials and Methods: Adult male Wistar rats (220±30 gr) were randomly divided into four groups (n=7 in each). Group 1 as control received normal saline by gavage, group 2 received CP (6.1 mg/kg/day, i.p.) for a period of 50 days. Group 3 received American ginseng (500 mg/kg/day) by gavage. Group 4 received American ginseng (500 mg/kg/day) 1h prior to the administration of CP in the equal dose of group 2. The animals scarified one day after the last treatment and the effects of American ginseng on the sperm vital parameters, testicular functions, biochemical factors, and structural malformations evaluated.
Results: Serum testosterone concentration was significantly decreased whereas the level of malondialdehyde and DNA damage were significantly increased in animals of CP group (p<0.01). Co-administration of American ginseng reversed these parameters and improved recovery in CP+ginseng group. In addition, seminiferous tubules of testis severely damaged in the CP group but ginseng improved histologic changes in CP+ginseng group.
Conclusion: The findings confirmed the protective effects of American ginseng on toxicity induced by CP in the reproductive system of male rats.
Type of Study: Original Article |
References
1. Junyoung Jo, Jerng UM. The effects of traditional Korean medicine in infertile male patients with poor semen quality: A retrospective study. Eur J Integr Med 2016; 8: 36-40. [DOI:10.1016/j.eujim.2016.01.007]
2. Zini A, Bielecki R, Phang D, Zenzes MT. Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil Steril 2001; 75: 674-677. [DOI:10.1016/S0015-0282(00)01796-9]
3. Kopalli SR, Won YJ, Hwang SY, Cha KM, Kim SY, Han CK, et al. Korean red ginseng protects against doxorubicin-induced testicular damage: An experimental study in rats. J Func Foods 2016; 20: 96-107. [DOI:10.1016/j.jff.2015.10.020]
4. Carrell DT, Liu L. Altered protamine 2 expression is uncommon in donors of known fertility, but common among men with poor fertilizing capacity, and may reflect other abnormalities of spermiogenesis. J Androl 2001; 22: 604-610.
5. Ludeman SM. The chemistry of the metabolites of cyclophosphamide. Curr Pharm Des 1999; 5: 627-643.
6. Crook TR, Souhami RL, McLean AE. Cytotoxicity, DNA cross-linking, and single strand breaks induced by activated cyclophosphamide and acrolein in human leukemia cells. Cancer Res 1986; 46: 5029-5034.
7. Anderson D, Bishop JB, Garner RC, Ostrosky-Wegman P, Selby PB. Cyclophosphamide: review of its mutagenicity for an assessment of potential germ cell risks. Mutat Res 1995; 330: 115-181. [DOI:10.1016/0027-5107(95)00039-L]
8. Kim KT, Yoo KM, Lee JW, Eom SH, Hwang IK, Lee CY. Protective effect of steamed American ginseng (Panax quinquefolius L.) on V79-4 cells induced by oxidative stress. J Ethnopharmacol 2007; 111: 443-450. [DOI:10.1016/j.jep.2007.01.004]
9. Wang L, Hao J, Hu J, Pu J, Lü Z, Zhao L, et al. Protective effects of ginsenosides against bisphenol a-induced cytotoxicity in 15P‐1 sertoli cells via extracellular signal-regulated kinase 1/2 signalling and antioxidant mechanisms. Basic Clin Pharmacol Toxicol 2012; 111: 42-49. [DOI:10.1111/j.1742-7843.2012.00857.x]
10. Moyad MA, Park K. What do most erectile dysfunction guidelines have in common? No evidence-based discussion or recommendation of heart-healthy lifestyle changes and/or Panax ginseng. Asian J Androl 2012; 14: 830-841. [DOI:10.1038/aja.2012.82]
11. Hosseini A, Ghaderi Pakdel F, Ahmadi A, Zare S. Beneficial effects of american ginseng on epididymal sperm analyses in cyclophosphamide treated rats. Cell J 2012; 14: 116-121.
12. Organisation WH. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. London; Cambridge university press, 1999.
13. Porter KL, Shetty G, Meistrich ML. Testicular edema is associated with spermatogonial arrest in irradiated rats. Endocrinology 2006; 147: 1297-1305. [DOI:10.1210/en.2005-0890]
14. Hess RA, Linder RE, Strader LF, Perreault SD. Acute effects and long‐term sequelae of 1, 3‐dinitrobenzene on male reproduction in the rat ii. quantitative and qualitative histopathology of the testis. J Androl 1988; 9: 327-342. [DOI:10.1002/j.1939-4640.1988.tb01059.x]
15. Narayana K, D'Souza UJ, Sanyal AK, Rao KP. 5-Fluorouracil (5-FU) induces the formation of giant cells and sloughing of seminiferous epithelium in the rat testis. Indian J Physiol Pharmacol 2000; 44: 317-322.
16. Narayana K, Prashanthi N, Nayanatara A, Kumar HH, Abhilash K, Bairy KL. Effects of methyl parathion (o, o-dimethyl o-4-nitrophenyl phosphorothioate) on rat sperm morphology and sperm count, but not fertility, are associated with decreased ascorbic acid level in the testis. Mutat Res 2005; 588: 28-34. [DOI:10.1016/j.mrgentox.2005.08.012]
17. Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal. Methods Enzymol 1990; 186: 407-421. [DOI:10.1016/0076-6879(90)86134-H]
18. Morris ID. Sperm DNA damage and cancer treatment1. Int J Androl 2002; 25: 255-261. [DOI:10.1046/j.1365-2605.2002.00372.x]
19. Elangovan N, Chiou T-J, Tzeng W-F, Chu S-T. Cyclophosphamide treatment causes impairment of sperm and its fertilizing ability in mice. Toxicology 2006; 222: 60-70. [DOI:10.1016/j.tox.2006.01.027]
20. Higuchi H, Nakaoka M, Kawamura S, Kamita Y, Kohda A, Seki T. Application of computer-assisted sperm analysis system to elucidate lack of effects of cyclophosphamide on rat epididymal sperm motion. J Toxicol Sci 2001; 26: 75-83. [DOI:10.2131/jts.26.75]
21. Katoh C, Kitajima S, Saga Y, Kanno J, Horii I, Inoue T. Assessment of quantitative dual-parameter flow cytometric analysis for the evaluation of testicular toxicity using cyclophosphamide-and ethinylestradiol- treated rats. J Toxicol Sci 2002; 27: 87-96. [DOI:10.2131/jts.27.87]
22. Jang DJ, Lee MS, Shin BC, Lee YC, Ernst E. Red ginseng for treating erectile dysfunction: a systematic review. Br J Clin Pharmacol 2008; 66: 444-450. [DOI:10.1111/j.1365-2125.2008.03236.x]
23. de Andrade E, de Mesquita AA, Claro Jde A, De Andrade PM, Ortiz V, Paranhos M, et al. Study of the efficacy of Korean Red Ginseng in the treatment of erectile dysfunction. Asian J Androl 2007; 9: 241-244. [DOI:10.1111/j.1745-7262.2007.00210.x]
24. Leung KW, Wong AS. Ginseng and male reproductive function. Spermatogenesis 2013; 3: e26391. [DOI:10.4161/spmg.26391]
25. Yang WM, Park SY, Kim HM, Park EH, Park SK, Chang MS. Effects of panax ginseng on glial cell-derived neurotrophic factor (GDNF) expression and spermatogenesis in rats. Phytother Res 2011; 25: 308-311.
26. Sikka SC. Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. J Androl 2004; 25: 5-18. [DOI:10.1002/j.1939-4640.2004.tb02751.x]
27. Arumugam N, Sivakumar V, Thanislass J, Devaraj H. Effects of acrolein on rat liver antioxidant defense system. Indian J Exp Biol 1997; 35: 1373-1374.
28. Zhang D, Yasuda T, Yu Y, Zheng P, Kawabata T, Ma Y, et al. Ginseng extract scavenges hydroxyl radical and protects unsaturated fatty acids from decomposition caused by iron-mediated lipid peroxidation. Free Radic Biol Med 1996; 20: 145-150. [DOI:10.1016/0891-5849(95)02020-9]
29. Primakoff P, Myles DG. Penetration, adhesion, and fusion in mammalian sperm-egg interaction. Science 2002; 296: 2183-2185. [DOI:10.1126/science.1072029]
30. Sanocka D, Kurpisz M. Reactive oxygen species and sperm cells. Reprod Biol Endocrinol 2004; 2: 1-12. [DOI:10.1186/1477-7827-2-12]
31. Vernet P, Aitken RJ, Drevet JR. Antioxidant strategies in the epididymis. Mol Cell Endocrinol 2004; 216: 31-39. [DOI:10.1016/j.mce.2003.10.069]
32. Zhu B, Zheng YF, Zhang YY, Cao YS, Zhang L, Li XG, et al. Protective effect of L-carnitine in cyclophosphamide-induced germ cell apoptosis. J Zhejiang Univ Sci B 2015; 16: 780-787. [DOI:10.1631/jzus.B1500015]
33. Fischer MA, Willis J, Zini A. Human sperm DNA integrity: correlation with sperm cytoplasmic droplets. Urology 2003; 61: 207-211. [DOI:10.1016/S0090-4295(02)02098-8]
34. Barth AD, Bowman PA. The sequential appearance of sperm abnormalities after scrotal insulation or dexamethasone treatment in bulls. Can Vet J 1994; 35: 93-102.
35. Kosower Ns, Katayose H, Yanagimachi R. Thiol-disulfide status and acridine orange fluorescence of mammalian sperm nuclei. J Androl 1992; 13: 342-348.
36. Zini A, De Lamirande E, Gagnon C. Low levels of nitric oxide promote human sperm capacitation in vitro. J Androl 1995; 16: 424-431.
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