Volume 16, Issue 12 (December 2018)                   IJRM 2018, 16(12): 783-790 | Back to browse issues page

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Shariati F, Favaedi R, Ramazanali F, Ghoraeian P, Afsharian P, Aflatoonian B, et al . Increased expression of stemness genes REX-1, OCT4, NANOG, and SOX-2 in women with ovarian endometriosis versus normal endometrium: A case-control study. IJRM 2018; 16 (12) :783-790
URL: http://ijrm.ir/article-1-1334-en.html
Increased expression of stemness genes REX-1, OCT4, NANOG, and SOX-2 in women with ovarian endometriosis versus normal endometrium: A case-control study
Fatameh Shariati1 , Raha Favaedi2 , Fariba Ramazanali3 , Pegah Ghoraeian4 , Parvaneh Afsharian2 , Behrouz Aflatoonian5 , Reza Aflatoonian3 , Maryam Shahhoseini * 6
1- Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
2- Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
3- Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
4- Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
5- Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
6- Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , m.shahhoseini@royaninstitute.org
Abstract:   (2665 Views)
Background: Endometriosis is a common, chronic inflammatory disease which is defined as an overgrowth of endometrial tissue outside the uterine cavity. The etiology of this disease is complex and multifactorial but there is a strong evidence that supports the presence of endometrial stem cells and their possible involvement in endometriosis.
Objective: In this study, we analyzed the mRNA expression of REX-1 stemness gene and reconsidered three other stemness genes SOX-2, NANOG, OCT-4 in women with endometriosis compared to normal endometrium.
Materials and Methods: Ten ectopic and ten eutopic tissue samples along with 23 normal endometrium specimens were recruited in this study. The expression levels of OCT-4, NANOG, SOX-2, and REX-1 genes were evaluated by the quantitative real-time polymerase chain reaction.
Results: The transcription levels of OCT-4, NANOG, and SOX-2 mRNA were significantly increased in ectopic lesions compared with eutopic and control group (p = 0.041, p = 0.035, p = 0.048), although the REX-1 mRNA increase was not significant between endometriosis and control groups. Also, there were differences in the expression level of these genes in normal endometrium during the menstrual cycles (p = 0.031, p = 0.047, p = 0.031).
Conclusion: Based on our data, we confirm the dynamic role of stemness genes in proliferation and growth of normal endometrium during the menstrual cycle and conclude that differential expression 
Keywords: Endometriosis, Stemness genes
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Type of Study: Original Article |
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