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Volume 18, Issue 10 (October 2020)
IJRM 2020, 18(10): 877-884 |
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Ramazani N, Ostadsharif M, Nayeri H. Association of BsmI variant of vitamin D receptor gene with polycystic ovary syndrome: A case-control study. IJRM 2020; 18 (10) :877-884
URL: http://ijrm.ir/article-1-1441-en.html
URL: http://ijrm.ir/article-1-1441-en.html
1- Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
2- Department of Medical Basic Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. Department of Medical Biotechnology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. , maryam.ostadsharif@gmail.com
2- Department of Medical Basic Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. Department of Medical Biotechnology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. , maryam.ostadsharif@gmail.com
Abstract: (1873 Views)
Background: Polycystic ovarian syndrome (PCOS) is an endocrine disorder that affects women's fertility and causes alterations such as obesity, insulin resistance, menstrual irregularities, and polycystic ovaries. The results of the studies show that the issue of vitamin D and vitamin D receptor (VDR) is controversial for PCOS susceptibility.
Objective: To investigate the association of BsmI polymorphism in the VDR gene with metabolic parameters in obese PCOS women.
Materials and Methods: In this case-control study, 38 obese subjects with PCOS and 40 unrelated obese individuals were evaluated to determine the allelic and genotypic frequency of BsmI variant by Polymerase Chain Reaction Restriction Fragment Length Polymorphism method. Body Mass Index, parathyroid hormone, phosphorus, and calcium were evaluated in all participants.
Results: BsmI (rs1544410), (A/G) AA, AG, GG, A, and G percentage of genotypic/allelic frequencies were 65.8, 26.3, 7.9, 78.9, and 21.1 in cases and 57.5, 40, 2.5, 77.5, and 22.5 in controls, respectively. Statistical analysis revealed that the differences in genotypic (p = 0.31)/allelic (p = 0.83) frequencies and dominant (p = 0.45)/recessive (p = 0.35) models between the cases and controls were not significant. This study indicates no association between the BsmI genotypes and metabolic parameters.
Conclusion: It can be concluded that VDR BsmI (rs1544410) Intron 8 (A>G) was not associated with obesity along with PCOS susceptibility in the studied groups.
Objective: To investigate the association of BsmI polymorphism in the VDR gene with metabolic parameters in obese PCOS women.
Materials and Methods: In this case-control study, 38 obese subjects with PCOS and 40 unrelated obese individuals were evaluated to determine the allelic and genotypic frequency of BsmI variant by Polymerase Chain Reaction Restriction Fragment Length Polymorphism method. Body Mass Index, parathyroid hormone, phosphorus, and calcium were evaluated in all participants.
Results: BsmI (rs1544410), (A/G) AA, AG, GG, A, and G percentage of genotypic/allelic frequencies were 65.8, 26.3, 7.9, 78.9, and 21.1 in cases and 57.5, 40, 2.5, 77.5, and 22.5 in controls, respectively. Statistical analysis revealed that the differences in genotypic (p = 0.31)/allelic (p = 0.83) frequencies and dominant (p = 0.45)/recessive (p = 0.35) models between the cases and controls were not significant. This study indicates no association between the BsmI genotypes and metabolic parameters.
Conclusion: It can be concluded that VDR BsmI (rs1544410) Intron 8 (A>G) was not associated with obesity along with PCOS susceptibility in the studied groups.
Type of Study: Original Article |
Subject:
Reproductive Genetics
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