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Volume 17, Issue 5 (May 2019 2019)
IJRM 2019, 17(5): 337-348 |
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Bagherpour H, Malekshah A K, Talebpour Amiri F, Azadbakht M. Protective effect of green tea extract on the
deltamethrin-induced toxicity in mice testis:
An experimental study. IJRM 2019; 17 (5) :337-348
URL: http://ijrm.ir/article-1-1517-en.html
URL: http://ijrm.ir/article-1-1517-en.html
Hoda Bagherpour1 
, Abbasali Karimpour Malekshah * 2, Fereshteh Talebpour Amiri1 , Mohammad Azadbakht3
, Abbasali Karimpour Malekshah * 2, Fereshteh Talebpour Amiri1 , Mohammad Azadbakht3
1- Department of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran
2- Department of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
3- Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
2- Department of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
3- Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Abstract: (2828 Views)
Background: Deltamethrin (DM) is one of the environmental factors that can have destructive effects on the male fertility. Green tea (GT) as a medicinal herb, has antioxidant property.
Objective: The present study investigated the protective role of GT extract in improving the harmful effects of DM on the testis.
Materials and Methods: In this experimental study, 35 adult male mice (25–30 gr) were divided in to five groups (n = 7/each). The control group received only normal saline.
Sham received 0.2 ml corn oil. Green tea group received only GT of 150 mg/kg. bw; deltamethrin group received the DM at a dose of 0.6 mg/kg. bw; GT + DM received both GT and DM. The effect of GT was assessed by measuring oxidative stress markers, sperm parameters, histological and immunohistochemical analysis.
Results: The results showed that thecount and motility of spermatozoa, testosterone, and Malondialdehyde significantly decreased (p < 0.001) and the abnormal spermatozoa increased (p < 0.001) in DM group compared to control group. Moreover, enhanced caspase-3expression and apoptosis were observed in DM-treated mice compared to control group. Histologically, DM with a degenerative effect on testicular tissue reduced
the spermatogenesis progenitor cells. The epithelial height and the diameter of the seminiferous tubules were also reduced in the DM group. Treatment with GTin the DMtreated mice significantly improved these changes.
Conclusion: With these findings, it was concluded that the GT treatment with antioxidant activity and anti-apoptotic property could protect the testicular injury induced by DM.
Objective: The present study investigated the protective role of GT extract in improving the harmful effects of DM on the testis.
Materials and Methods: In this experimental study, 35 adult male mice (25–30 gr) were divided in to five groups (n = 7/each). The control group received only normal saline.
Sham received 0.2 ml corn oil. Green tea group received only GT of 150 mg/kg. bw; deltamethrin group received the DM at a dose of 0.6 mg/kg. bw; GT + DM received both GT and DM. The effect of GT was assessed by measuring oxidative stress markers, sperm parameters, histological and immunohistochemical analysis.
Results: The results showed that thecount and motility of spermatozoa, testosterone, and Malondialdehyde significantly decreased (p < 0.001) and the abnormal spermatozoa increased (p < 0.001) in DM group compared to control group. Moreover, enhanced caspase-3expression and apoptosis were observed in DM-treated mice compared to control group. Histologically, DM with a degenerative effect on testicular tissue reduced
the spermatogenesis progenitor cells. The epithelial height and the diameter of the seminiferous tubules were also reduced in the DM group. Treatment with GTin the DMtreated mice significantly improved these changes.
Conclusion: With these findings, it was concluded that the GT treatment with antioxidant activity and anti-apoptotic property could protect the testicular injury induced by DM.
Type of Study: Original Article |
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