International Journal of
Reproductive Biomedicine
Sat, Apr 20, 2024
[Archive]
Volume 18, Issue 9 (September 2020)
IJRM 2020, 18(9): 785-794 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mazaheri F, Kamali K, Kalantar S M, Ziya N, Khoradmehr A, Anvari M. Effects of phosalone plant pesticide on sperm parameters and sexual hormone levels in Wistar rats: An experimental study. IJRM 2020; 18 (9) :785-794
URL: http://ijrm.ir/article-1-1549-en.html
URL: http://ijrm.ir/article-1-1549-en.html
Fahime Mazaheri1 
, Kazem Kamali * 2, Seyed Mehdi Kalantar3 , Noosha Ziya4 , Arezoo Khoradmehr5 , Morteza Anvari3
, Kazem Kamali * 2, Seyed Mehdi Kalantar3 , Noosha Ziya4 , Arezoo Khoradmehr5 , Morteza Anvari3
1- Department of Biochemistry, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran. Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Department of Arid land and Desert Management, Faculty of Natural Resources, Yazd University, Yazd, Iran. , kkamali@yazd.ac.ir
3- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Islamic Azad University of Shahrekord , Iran.
5- The Persian Gulf Marine Biotechnology Research Center, the Persian Gulf Biomedical Sciences Research Institute Bushehr University of Medical Sciences, Bushehr, Iran.
2- Department of Arid land and Desert Management, Faculty of Natural Resources, Yazd University, Yazd, Iran. , kkamali@yazd.ac.ir
3- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Islamic Azad University of Shahrekord , Iran.
5- The Persian Gulf Marine Biotechnology Research Center, the Persian Gulf Biomedical Sciences Research Institute Bushehr University of Medical Sciences, Bushehr, Iran.
Abstract: (1764 Views)
Background: Phosalone is an organophosphate insecticide, applied to control of plant pests. This compound has various side effects because it acts as an acetyl cholinesterase enzyme inhibitor.
Objective: To investigate the effects of phosalone on the sperm parameters of and levels of sex hormones in adult male rats.
Materials and Methods: In this experimental study, 16 adult (8-12 wk) male Wister rates (weighing 220-280 gr) were randomly assigned into 4 groups (n= 4/each). Group 1 (control) received only routine adequate water and food; Group 2, 3, and 4 received different low doses of phosalone (60, 90, and 120 mg/kg respectively). The rats were weighed and anesthetized after 48 days. Sperm parameters including number, motility, and viability as well as sex hormones (such as Luteinizing Hormone, Follicle Stimulating Hormone, and testosterone) were evaluated and compared after removing the epididymis tail.
Results: Our results showed that phosalone decreased sperm motility, viability, and number in a dose-dependent manner. The level of FSH and LH was increased, and testosterone was decreased. Also, depending on the dose, phosalone decrease sperm motility and viability (p ≤ 0.001), while the level of FSH and LH was increased and testosterone was decreased (p = 0.861).
Conclusion: Phosalone has negative effects on reproductive indices in male rats and can cause serious damage and decrease the number and sperms motility. It can also cause infertility due to changing the concentration of hormones.
Objective: To investigate the effects of phosalone on the sperm parameters of and levels of sex hormones in adult male rats.
Materials and Methods: In this experimental study, 16 adult (8-12 wk) male Wister rates (weighing 220-280 gr) were randomly assigned into 4 groups (n= 4/each). Group 1 (control) received only routine adequate water and food; Group 2, 3, and 4 received different low doses of phosalone (60, 90, and 120 mg/kg respectively). The rats were weighed and anesthetized after 48 days. Sperm parameters including number, motility, and viability as well as sex hormones (such as Luteinizing Hormone, Follicle Stimulating Hormone, and testosterone) were evaluated and compared after removing the epididymis tail.
Results: Our results showed that phosalone decreased sperm motility, viability, and number in a dose-dependent manner. The level of FSH and LH was increased, and testosterone was decreased. Also, depending on the dose, phosalone decrease sperm motility and viability (p ≤ 0.001), while the level of FSH and LH was increased and testosterone was decreased (p = 0.861).
Conclusion: Phosalone has negative effects on reproductive indices in male rats and can cause serious damage and decrease the number and sperms motility. It can also cause infertility due to changing the concentration of hormones.
Type of Study: Original Article |
Subject:
Reproductive Biology
References
1. Bretveld RW, Thomas CMG, Scheepers PTJ, Zielhuis GA, Roeleveld N. Pesticide exposure: the hormonal function of the female reproductive system disrupted? Reprod Biol Endocrinol 2006; 4: 30-43. [DOI:10.1186/1477-7827-4-30] [PMID] [PMCID]
2. Tabb MM, Blumberg B. New modes of action for endocrine-disrupting chemicals. Mol Endocrinol 2006; 20: 475-482. [DOI:10.1210/me.2004-0513] [PMID]
3. Taib IS, Budin SB, Ghazali AR, Jayusman PA, Mohamed J. Fenitrothion alters sperm characteristics in rats: ameliorating effects of palm oil tocotrienol-rich fraction. Exp Anim 2014; 63: 383-393. [DOI:10.1538/expanim.14-0016] [PMID] [PMCID]
4. Kidd SA, Eskenazi B, Wyrobek AJ. Effects of male age on semen quality and fertility: A review of the literature. Fertil Steril 2001; 75: 237-248. [DOI:10.1016/S0015-0282(00)01679-4]
5. Sharma B, Sharma P, Joshi S. Effect of an organophosphorus insecticide di chorovos on the reproductive performance in male rat. Eur J Biomed Pharm Sci 2016; 8: 403-409.
6. Sujatha R, Chitra KC, Latchoumycandane C, Mathur PP. Effect of lindane on testicular antioxidant system and steroidogenic enzymes in adult rats. Asian J Androl 2001; 3: 135-138.
7. Aktar WM, Sengupta D, Chowdhury A. Impact of pesticides use in agriculture: their benefits and hazards. Interdiscip Toxicol 2009; 2: 1-12. [DOI:10.2478/v10102-009-0001-7] [PMID] [PMCID]
8. Larsen SB, Giwercman A, Spanò M, Bonde JP. A longitudinal study of semen quality in pesticide spraying Danish farmers. The ASCLEPIOS study group. Reprod Toxicol 1998; 12: 581-589. [DOI:10.1016/S0890-6238(98)00047-1]
9. Nithya R, Elango V. Pesticide effect in male hormones and antioxidant status in male albino rats. J Acad Indus Res 2015; 4: 140-143.
10. Fattahi E, Parivar K, Jorsaraei SG, Moghadamnia AA. The effects of diazinon on testosterone, FSH and LH levels and testicular tissue in mice. Iran J Reprod Med 2009; 7: 59-64.
11. Toppari J, Larsen JC, Christiansen P, Giwercman A, Grandjean P, Guillette Jr LJ, et al. Male reproductive health and environmental xenoestrogens. Environ Health Perspect 1996; 104 (suppl.): 741-803. [DOI:10.1289/ehp.96104s4741] [PMID] [PMCID]
12. Okamura A, Kamijima M, Shibata E, Ohtani K, Takagi K, Ueyama J, et al. A comprehensive evaluation of the testicular toxicity of dichlorvos in Wistar rats. Toxicology 2005; 213: 129-137. [DOI:10.1016/j.tox.2005.05.015] [PMID]
13. Saenphet K, Jitjaingam A, Chaiyapo M. Reproductive toxicity test of plant-derived insecticide in male rats. Chiang Mai J Sci 2015; 42: 816-821.
14. Talebi AR, Khalili MA, Nahangi H, Abbasi A, Anvari M. Evaluation of epididymal necrospermia following experimental chronic spinal cord injury in rat. Iran J Reprod Med 2007; 5: 171-176.
15. Farag AT, Eweidah MH, El-Okazy AM. Reproductive toxicology of acephate in male mice. Reprod Toxicol 2000; 14: 457-462. [DOI:10.1016/S0890-6238(00)00094-0]
16. Mnif W, Ibn Hadj Hassine A, Bouaziz A, Bartegi A, Thomas O, Roig B. Effect of Endocrine disruptor pesticides. Int J Environ Res Public Health 2011; 8: 2265-2303. [DOI:10.3390/ijerph8062265] [PMID] [PMCID]
17. Joshi SC, Sharma P. Male reproductive toxicity of organophosphorous compounds: a review. Toxicol Environment Chem 2011; 93: 1486-1507. [DOI:10.1080/02772248.2011.581874]
18. Ambali SF, Akanbi DO, Shittu M, Giwa A, Oladipo OO, Olusegun Ayo J. Chlorpyrifos-induced clinical, haematological and biochemical changes in Swiss albino mice: mitigating effect by co-administration of vitamins C and E. Life Science Journal 2010; 7: 37-44.
19. Joshi SC, Mathur R, Gulati N. Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rat. Toxicol Ind Health 2007; 23: 439-444. [DOI:10.1177/0748233707080908] [PMID]
20. Jorgenson JL. Aldrin and dieldrin: a review of research on their production, environmental deposition and fate, bioaccumulation, toxicology, and epidemiology in the United States. Environ Health Perspect 2001; 109 (suppl.): 113-139. [DOI:10.1289/ehp.01109s1113] [PMID] [PMCID]
21. Hanke WO, Jurewicz J. The risk of adverse reproductive and developmental disorders due to occupational pesticide exposure: an overview of current epidemiological evidence. Int J Occup Med Environment Health 2004; 17: 223-243.
22. Geng X, Shao H, Zhang Z, Ng JC, Peng C. Malathion-induced testicular toxicity is associated with spermatogenic apoptosis and alterations in testicular enzymes and hormone levels in male Wistar rats. Environ Toxicol Pharmacol 2015; 39: 659-667. [DOI:10.1016/j.etap.2015.01.010] [PMID]
23. Farag AT, Radwan AH, Sorour F, El Okazy A, El-Agamy ES, El-Sebae AE. Chlorpyrifos induced reproductive toxicity in male mice. Reprode Toxicol 2010; 29: 80-85. [DOI:10.1016/j.reprotox.2009.10.003] [PMID]
24. Colborn T, Vom Saal FS, Soto AM. Developmental effects of endocrine-disrupting chemicals in wildlife and humans. Environ Health Perspect 1993; 101: 378-384. [DOI:10.1289/ehp.93101378] [PMID] [PMCID]
25. Jasuja ND, Sharma P, Joshi SC. A comprehensive effect of acephate on cauda epididymis and accessory sex organs of male rats. Afr J Pharm Pharmacol 2013; 7: 1560-1567. [DOI:10.5897/AJPP2013.0039]
26. Adamkovicova M, Toman R, Martiniakova M, Omelka R, Babosova R, Krajcovicova V, et al. Sperm motility and morphology changes in rats exposed to cadmium and diazinon. Reprod Biol Endocrinol 2016; 14: 42-48. [DOI:10.1186/s12958-016-0177-6] [PMID] [PMCID]
27. Slimani S, Boulakoud MS, Abdennour C. Pesticide exposure and reproductive biomarkers among male farmers from north-east Algeria. Ann Biol Res 2011; 2: 290-297.
28. Patrick-Iwuanyanwu K, Udowelle NA, Okereke CJ. Testicular toxicity and sperm quality following exposure to Solignum: A Permethrin-containing wood preservative in adult male Wistar rats. J Interdiscipl Histopathol 2016; 4: 13-16. [DOI:10.5455/jihp.20151125081952]
29. Olorunshola KV, Achie LN, Akpomiemie ML. Ascorbic acid ameliorates toxic effects of chlopyrifos on testicular functions of albino rats. Br J Pharmacol Toxicol 2011; 2: 262-269.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
