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Volume 18, Issue 8 (August 2020)
IJRM 2020, 18(8): 667-682 |
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Mehdikhani H, Shariati M, Forouzanfar M, Hosseini S E. Protective effects of Ceratonia siliqua extract on protamine gene expression, testicular function and testicular histology in doxorubicin-treated adult rats. IJRM 2020; 18 (8) :667-682
URL: http://ijrm.ir/article-1-1650-en.html
URL: http://ijrm.ir/article-1-1650-en.html
1- Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2- Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran. , mehrdadshariati@hotmail.com
3- Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.
4- Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
2- Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran. , mehrdadshariati@hotmail.com
3- Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.
4- Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
Abstract: (1956 Views)
Abstract
Background: Spermatogenesis is a complex process under the influence of many different genes.
Objective: This study was to investigate the possible effects of Ceratonia siliqua hydroalcoholic extract (CSHAE) on protamine gene expression, testicular function and testicular histology in doxorubicin-treated rats.
Materials and Methods: 56 adult male rats (210± 10 g) were divided into 7 groups (n=8/each). The control group left untreated; the sham group received 0.3ml distilled water intraperitoneally; The negative control group received 3 mg/kg doxorubicin, intraperitoneally, once a week for 28 days; the positive control group received 600 mg/kg of CSHAE, orally, for 48 days; the experimental groups 1, 2 and 3 received 150, 300 and 600 mg/kg of CSHAE, orally, respectively for 48 days, as well as 3 mg/kg doxorubicin once a week for 28 days. Hematoxylin-eosin staining was used in histological study of testes, and enzyme-linked immunosorbent assay method was used in measuring serum levels of testosterone. Protamine gene expression was determined by Real Time PCR method.
Results: Mean body weight (p=0.009), testicular weight (p<0.001), testicular volume (p<0.001), testosterone level (p=0.022), the count of Leydig (p=0.014), spermatogonia (p =0.046), spermatocyte (p =0.025), and spermatid (p=0.006) cells, as well as protamine gene expression (p=0.008) were significantly increased in experimental group 2 compared to the negative control group. The testicular tissue regeneration was observed in the experimental group 2.
Conclusion: CSHAE has protective effect on doxorubicin-induced testicular injuries.
Background: Spermatogenesis is a complex process under the influence of many different genes.
Objective: This study was to investigate the possible effects of Ceratonia siliqua hydroalcoholic extract (CSHAE) on protamine gene expression, testicular function and testicular histology in doxorubicin-treated rats.
Materials and Methods: 56 adult male rats (210± 10 g) were divided into 7 groups (n=8/each). The control group left untreated; the sham group received 0.3ml distilled water intraperitoneally; The negative control group received 3 mg/kg doxorubicin, intraperitoneally, once a week for 28 days; the positive control group received 600 mg/kg of CSHAE, orally, for 48 days; the experimental groups 1, 2 and 3 received 150, 300 and 600 mg/kg of CSHAE, orally, respectively for 48 days, as well as 3 mg/kg doxorubicin once a week for 28 days. Hematoxylin-eosin staining was used in histological study of testes, and enzyme-linked immunosorbent assay method was used in measuring serum levels of testosterone. Protamine gene expression was determined by Real Time PCR method.
Results: Mean body weight (p=0.009), testicular weight (p<0.001), testicular volume (p<0.001), testosterone level (p=0.022), the count of Leydig (p=0.014), spermatogonia (p =0.046), spermatocyte (p =0.025), and spermatid (p=0.006) cells, as well as protamine gene expression (p=0.008) were significantly increased in experimental group 2 compared to the negative control group. The testicular tissue regeneration was observed in the experimental group 2.
Conclusion: CSHAE has protective effect on doxorubicin-induced testicular injuries.
Type of Study: Original Article |
Subject:
Reproductive Andrology
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