Volume 17, Issue 10 (October 2019)                   IJRM 2019, 17(10): 717-726 | Back to browse issues page

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Azizi P, Soleimani Mehranjani M. The effect of green tea extract on the sperm parameters and histological changes of testis in rats exposed to para-nonylphenol. IJRM 2019; 17 (10) :717-726
URL: http://ijrm.ir/article-1-1694-en.html
The effect of green tea extract on the sperm parameters and histological changes of testis in rats exposed to para-nonylphenol
Parisa Azizi1 , Malek Soleimani Mehranjani * 2
1- Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.
2- Department of Biology, Faculty of Sciences, Arak University, Arak, Iran , m-Soleimani@araku.ac.ir
Abstract:   (5304 Views)
Abstract

Background: Para-nonylphenol (p-NP), an environmental contaminant, can generate free radicals that disturbs the reproductive properties. Green tea extract (GTE) is an antioxidant which may prevent the adverse effects of free radicals.

Objective: The aim was to investigate the effect of GTE on sperm parameters and testis tissue in p-NP-treated rats.

Materials and Methods: 24 adult male Wistar rats (215 ± 20 gr) were randomly divided into four groups (n = 6/each) – including control, p-NP (200 mg/kg/day), GTE (200 mg/kg/day), and p-NP + GTE – and orally treated for 56 days. The right testes and left caudal epididymis were used to evaluate selected parameters. In addition, the concentration of serum malondialdehyde was calculated.

Results: A significant decrease in the sperm number, motility, viability and morphology (p < 0.001) was observed in the rats treated with p-NP compared to the control ones. The diameter of seminiferous tubules (p < 0.001), thickness of germinal epithelium (p = 0.018), total volume of testis (p = 0.009), volume of seminiferous tubules (p < 0.001), and testis weight (p = 0.017) decreased in the p-NP group in contrast with the other groups. Moreover, a significant increase of the malondialdehyde concentration was seen in the p-NP group when compared with the controls (p = 0.043). The majority of adverse effects of p-NP could be recovered following the administration of GTE.

Conclusion: It seems GTE can be used as a potent antioxidant in the case of p-NP toxication.
Keywords: Green tea extract, Para-nonylphenol, Sperm, Testis, Rat.
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Type of Study: Original Article |
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