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Reproductive Biomedicine
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Volume 8, Issue 4 (7-2010)
IJRM 2010, 8(4): 173-178 |
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Nowroozi M R, Radkhah K, Ranjbaran A, Ghaffari S R, Sedighi Gilani M A, Gourabi H. Is karyotyping and Y chromosome microdeletion study necessary in men candidate for ICSI?. IJRM 2010; 8 (4) :173-178
URL: http://ijrm.ir/article-1-194-en.html
URL: http://ijrm.ir/article-1-194-en.html
Mohammad Reza Nowroozi * 
1, Keivan Radkhah2 , Alireza Ranjbaran2 , Saeed Reza Ghaffari3 , Mohammad Ali Sedighi Gilani4 , Hamid Gourabi5
1, Keivan Radkhah2 , Alireza Ranjbaran2 , Saeed Reza Ghaffari3 , Mohammad Ali Sedighi Gilani4 , Hamid Gourabi5
1- Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran , mrnowroozi@yahoo.com
2- Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Genetics, Tehran University of Medical Sciences, Tehran, Iran
4- Department of Urology, Rooyan Institute, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Genetics, Rooyan Institute, Tehran, Iran
2- Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Genetics, Tehran University of Medical Sciences, Tehran, Iran
4- Department of Urology, Rooyan Institute, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Genetics, Rooyan Institute, Tehran, Iran
Abstract: (2520 Views)
Background: The sperm count and function may be affected by karyotype abnormalities or microdeletion in Y chromosome. These genetic abnormalities can probably transmit to the children.
Objective: In this study, we tried to determine the frequency of karyotype abnormalities and Y chromosome microdeletions in severe oligospermic or azoospermic men who fathered sons by ICSI.
Materials and Methods: This study comprised of fathers who had at least a son with ICSI due to severe oligospermia or azoospermia. General examinations were done and blood sample were obtained for karyotype and Y chromosome studies.
Results: The total of 60 fathers was evaluated along with their 70 sons. The mean duration of infertility was 8.7 years and the sons were 2.4 years in average at the time of examination. The mean age of neonates at the time of delivery was 33 weeks; 42.9% were delivered prematurely; and 40.5% of them were twins. 8.6% of the sons had hypospadiasis and 7.1% had UDT. Most of the side effects were due to prematurity. In total 6 of fathers had karyotype anomaly, meanwhile 4 of their sons had also karyotype anomaly. Only one son had karyotype anomaly without affected father. No case of Y chromosome microdeletion was found in the fathers.
Conclusion: Y chromosome microdeletion is not prevalent in fathers with successful ICSI and it is not necessary to be analyzed before ICSI performance. Karyotype anomaly may transmit to the sons. All together ICSI is reliable and safe. Most of the complications are the result of premature delivery.
Objective: In this study, we tried to determine the frequency of karyotype abnormalities and Y chromosome microdeletions in severe oligospermic or azoospermic men who fathered sons by ICSI.
Materials and Methods: This study comprised of fathers who had at least a son with ICSI due to severe oligospermia or azoospermia. General examinations were done and blood sample were obtained for karyotype and Y chromosome studies.
Results: The total of 60 fathers was evaluated along with their 70 sons. The mean duration of infertility was 8.7 years and the sons were 2.4 years in average at the time of examination. The mean age of neonates at the time of delivery was 33 weeks; 42.9% were delivered prematurely; and 40.5% of them were twins. 8.6% of the sons had hypospadiasis and 7.1% had UDT. Most of the side effects were due to prematurity. In total 6 of fathers had karyotype anomaly, meanwhile 4 of their sons had also karyotype anomaly. Only one son had karyotype anomaly without affected father. No case of Y chromosome microdeletion was found in the fathers.
Conclusion: Y chromosome microdeletion is not prevalent in fathers with successful ICSI and it is not necessary to be analyzed before ICSI performance. Karyotype anomaly may transmit to the sons. All together ICSI is reliable and safe. Most of the complications are the result of premature delivery.
Type of Study: Original Article |
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