Volume 9, Issue 2 (7-2011)                   IJRM 2011, 9(2): 125-130 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Talaei-khozani T, Borzoei Z, Bahmanpour S, Zolghadr J, Dehbashi S, Zareh H R. Effects of sera taken from women with recurrent spontaneous abortion on sperm motility and apoptosis. IJRM 2011; 9 (2) :125-130
URL: http://ijrm.ir/article-1-214-en.html
Effects of sera taken from women with recurrent spontaneous abortion on sperm motility and apoptosis
Tahereh Talaei-khozani * 1, Zahra Borzoei2 , Soghra Bahmanpour2 , Jaleh Zolghadr3 , Sedigheh Dehbashi3 , Hamid Reza Zareh4
1- Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran , talaeit@sums.ac.ir
2- Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran
3- Department of Obstetrics and Gynecology, Shiraz University of Medical Sciences, Shiraz, Iran
4- Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:   (2581 Views)
Background: Recurrent spontaneous abortion impacts almost 1% of couples. The sera from women with unexplained recurrent spontaneous abortion (URSA) have toxic effects on embryos that grow in the uterus. Therefore, the abnormal condition of the uterus may also affect sperm qualities.
Objective: The objectives of this study were to search if these sera could induce DNA denaturation in sperm nuclei and also it could reduce sperm motility.
Materials and Methods: Sera of 20 women with URSA history and sera from 20 women with at least two healthy children were added to the sperms samples from 20 healthy men for 2 hours. The sperm motility was assessed after incubation with sera. The samples were stained with Tdt mediated dUTP nick end labeling (TUNEL) assay for DNA fragmentation. The samples were analyzed with flow cytometry and the percentage of the TUNEL positive sperms were calculated. The data were analyzed by t-test.
Results: The incubation of the sperm samples in sera with URSA lead to a decrease in the percentage of the motile sperm from 55% in control to 41% in the treated group, significantly (p=0.038). The percentage of the sperm with abnormal fragmented DNA increased after incubation with URSA (26.6%) compare to the control (21.2%); however, it was not significant.
Conclusion: It seems that sera from URSA patients could not induce a significant increase in the percentage of the sperms with nuclei contain DNA fragmentation. However, the sera of women with URSA could affect the fertility rate by reduction of the sperm motility
Keywords: Unexplained recurrent spontaneous abortion, Sperm, Apoptosis, Sperm motility
Full-Text [PDF 101 kb]   (484 Downloads) |   |   Full-Text (HTML)  (285 Views)  
Type of Study: Original Article |
References
1. Stirrat GM. Recurrent pregnancy loss I: definition and epidemiology. Lancet 1990; 336: 673-675. [DOI:10.1016/0140-6736(90)92159-F]
2. Coulam CB. Epidemiology of recurrent spontaneous abortion. Am J Reprod Immunol 1991; 26: 23-27. [DOI:10.1111/j.1600-0897.1991.tb00697.x]
3. Saxena P, Misro MM, Roy S, Chopra K, Sinha D, Nandan D, et al. Possible role of male factors in recurrent pregnancy loss. Indian J Physiol Pharmacol 2008; 52: 274-282.
4. Saxena P, Misro MM, Chaki SP, Chopra K, Roy S, Nandan D. Is abnormal sperm function an indicator among couples with recurrent pregnancy loss? Fertil Steril 2008; 90: 1854-1858. [DOI:10.1016/j.fertnstert.2007.09.031]
5. Gopalkrishnan K, Padwal V, Meherji PK, Gokral JS, Shah R, Juneja HS. Poor quality of sperm as it affects repeated early pregnancy loss. Arch Androl 2000; 45: 111-117. [DOI:10.1080/014850100418800]
6. Zorn B, Virant-Klun I, Drobni S, Sinkovec J, Meden-Vrtovec H. Male and female factors that influence ICSI outcome in azoospermia or aspermia. Reprd Biomed online 2009; 18: 168-176. [DOI:10.1016/S1472-6483(10)60252-0]
7. Zini A, Boman JM, Belzile E, Ciampi A. Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: systematic review and meta-analysis. Hum Reprod 2008; 23: 2663-2668. [DOI:10.1093/humrep/den321]
8. Gandini L, Lombardo F, Paoli D, Caruso F, Eleuteri P, Leter G, et al. Full-term pregnancies achieved with ICSI despite high levels of sperm chromatin damage. Hum Reprod 2004; 19: 1409-1417. [DOI:10.1093/humrep/deh233]
9. Bungum M, Humaidan P, Spano M, Jepson K, Bungum L, Giwercman A. The predictive value of sperm chromatin structure assay (SCSA) parameters for the outcome of intrauterine insemination, IVF and ICSI. Hum Reprod 2004; 19: 1401-1408. [DOI:10.1093/humrep/deh280]
10. Fernandez-Gonzalez R, Moreira PN, Perez-Crespo M, Sanchez-Martin M, Ramirez MA, Pericuesta E, et al: Long-term effects of mouse intracytoplasmic sperm injection with DNA-fragmented sperm on health and behavior of adult offspring. Biol Reprod 2008; 78: 761-772. [DOI:10.1095/biolreprod.107.065623]
11. Perez-Crespo M, Moreira P, Pintado B, Gutierrez-Adan A. Factors from damaged sperm affect its DNA integrity and its ability to promote embryo implantation in mice. J Androl 2008; 29: 47-54. [DOI:10.2164/jandrol.107.003194]
12. Ming-Huei Lin, Robert Kuo-Kuang Lee, Sheng-Hsiang Li, Chung-Hao Lu, Fang-Ju Sun, Yuh-Ming Hwu. Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic sperm injection, but might be related to spontaneous abortion rates. Fertil Steril 2008; 90: 352-359. [DOI:10.1016/j.fertnstert.2007.06.018]
13. Abir R, Zusman I, Ben Hur H, Yaffe P, Ornoy A. The effects of serum from women with miscarriages on the in vitro development of mouse preimplantation embryos. Acta Obstet Gynecol Scand 1990; 69: 27-33. [DOI:10.3109/00016349009021035]
14. Talaei-Khozani T, Aminizadeh N, Aliabadi E, Mesbah SF, Zolghadr J. lectin reactivity of expanded mouse blastocysts after exposure to sera from women with unexplained recuurent spontaneous abortion. Rep Toxicol. 2005; 20: 531-537. [DOI:10.1016/j.reprotox.2005.04.015]
15. Al-Hussein K, Al-Mukhalafi Z, Bertilsson PA, Jaroudi K, Shoukri M, Hollander j. Value of flow cytometric assay for the detection of antisperm antibodies in women with a history of recurrent abortion. Am J Reprod Immunol 2002; 47: 31-37. [DOI:10.1034/j.1600-0897.2002.1o013.x]
16. Clarke GN. Etiology of sperm immunity in women. Fertil Steril 2009: 91: 639-643. [DOI:10.1016/j.fertnstert.2007.11.045]
17. Haller K, Sikut A, Karro H, Uibo R, Salumets A. Circulating anti-follicle-stimulating hormone immunoglobulin A in women: a sperm-prone reaction of mucosal tolerance? Fertil Steril 2008; 90: 1253-1255. [DOI:10.1016/j.fertnstert.2007.08.073]
18. Carreau S, Delalande C, Galeraud-Denis I. Mammalian sperm quality and aromatase expression. Microsc Res Tech 2009; 72: 552-557. [DOI:10.1002/jemt.20703]
19. Walczak-Jedrzejowska R, Slowikowska-Hilczer J, Marchlewsk K, Oszukowska E, Kula K. During seminiferous tubule maturation testosterone and synergistic action of FSH with estradiol support germ cell survival while estradiol alone has pro-apoptotic effect. Folia Histochem Cytobiol 2007; 45: 59-64.
20. World Health Organization. Laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th Ed. Cambridge University Press; Cambridge; 1991.
21. Getpook C, Wirotkarun S. Sperm motility stimulation and preservation with various concentrations of follicular fluid. J Assist Reprod Genet 2007; 24: 425-428. [DOI:10.1007/s10815-007-9145-6]
22. Cerkiene Z, Eidukaite A, Usoniene A. Follicular fluid levels of interleukin-10 and interferon-gamma do not predict outcome of assisted reproductive technologies. Am J Reprod Immunol 2008; 59: 118-126. [DOI:10.1111/j.1600-0897.2007.00552.x]
23. Lampiao F, du Plessis SS. TNF-alpha and IL-6 affect human sperm function by elevating nitric oxide production. Reprod Biomed Online 2008; 17: 628-631. [DOI:10.1016/S1472-6483(10)60309-4]
24. Gupta S, Agarwal A, Banerjee J, Alvarez JG. The role of oxidative stress in spontaneous abortion and recurrent pregnancy loss: A systematic review. Obstet Gynecol Surv 2007; 62: 335-347. [DOI:10.1097/01.ogx.0000261644.89300.df]
25. Kao SH, Chao HT, Chen HW, Hwang TI, Liao TL, Wei YH. Increase of oxidative stress in human sperm with lower motility. Fertil Steril 2008; 89: 1183-1190. [DOI:10.1016/j.fertnstert.2007.05.029]
26. Allam JP , Fronhoffs F, Fathy A, Novak N, Oltermann I, Bieber T, et al. High percentage of apoptotic spermatozoa in ejaculates from men with chronic genital tract inflammation. Inter J Andrologica 2008; 40: 329-334. [DOI:10.1111/j.1439-0272.2008.00864.x]
27. Moskovtsev SI, Willis J, White J, Mullen JB. Sperm DNA damage: correlation to severity of semen abnormalities. Urology 2009; 74: 789-793. [DOI:10.1016/j.urology.2009.05.043]
28. Toy H, Camuzcuoglu H, Celik H, Erel O, Aksoy N. Assessment of serum paraoxonase and arylesterase activities in early pregnancy failure. Swiss Med Wkly 2009; 139: 76-81.
29. Aitken RJ, Koppers AJ. Apoptosis and DNA damage in human spermatozoa. Asian J Androl. 2010; 30: 36-42.
30. Gandini L, Lombardo F, Paoli D, Caponecchia L, Familiari G, Verlengia C, et al. study of apoptotic DNA fragmentation in human spermatozoa. Hum Repord 2000; 15: 830-839. [DOI:10.1093/humrep/15.4.830]
31. Oosterhuis GJE, Vermes I. apoptosis in human ejaculated spermatozoa. J Biol Regul Homeost 2004; 18: 115-119.
32. Shen HM, Dai J, Chia SE, Lim A, Ong CN. Detection of apoptotic alterations in sperm in subfertile patients and their correlation with sperm quality. Hum Reprod 2002; 17: 1266-1273. [DOI:10.1093/humrep/17.5.1266]
33. Coulam CB. Association between infertility and spontaneous abortion. Am J Reprod Immunol 1992; 27: 128-129. [DOI:10.1111/j.1600-0897.1992.tb00739.x]
34. Ferrari DA, Gilles PA, Klein NW, Nadler D, Weeks BS, Lammi-Keefe CJ, Hillman RE, et al. Rat embryo development on human sera is related to numbers of previous spontaneous abortions and nutritional factors. Am J Obstet Gynecol 1994; 170: 228-236. [DOI:10.1016/S0002-9378(94)70412-0]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb