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Reproductive Biomedicine
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Volume 20, Issue 12 (December 2022)
IJRM 2022, 20(12): 999-1006 |
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Bahrami Z, Daeifarshbaf N, Amjadi F, Aflatoonian R. The effects of hormonal changes on sperm DNA integrity in oligoasthenoteratospermia individuals: A case-control study. IJRM 2022; 20 (12) :999-1006
URL: http://ijrm.ir/article-1-2160-en.html
URL: http://ijrm.ir/article-1-2160-en.html
1- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Laleh IVF Clinic, Laleh Hospital, Tehran, Iran.
2- Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Infertility Center, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, Iran.
3- Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
4- Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Laleh IVF Clinic, Laleh Hospital, Tehran, Iran. , R.aflatoonian@royaninstitute.org
2- Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Infertility Center, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, Iran.
3- Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
4- Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Laleh IVF Clinic, Laleh Hospital, Tehran, Iran. , R.aflatoonian@royaninstitute.org
Abstract: (847 Views)
Abstract
Background: Hormonal imbalance is one of the important etiological factors for Oligoasthenoteratospermias (OAT).
Objective: This study aimed to evaluate the effects of hormonal changes including prolactin, TSH, testosterone, luteinizing hormone, follicle-stimulating hormone and anti-Mullerian hormone on sperm DNA fragmentation in normal men compared with OAT to design a clinical algorithm for the comprehensive study of male factor infertilities.
Materials and Methods: We consecutively selected 60 candidates referred to the infertility clinic to collect the semen and blood samples. Then, a terminal deoxynucleotidyl transferase dUTP nick end labeling test was performed to evaluate the sperm DNA fragmentation index (DFI). After semen analysis and DFI checking, they were classified into 4 groups consisting of normospermia and OAT men each with or without increased DFI. Hormone parameters were analyzed using enzyme-linked immunoassay.
Results: Follicle-stimulating hormone and luteinizing hormone levels showed positive correlations with DFI in a significant way (p ≤ 0.01), while testosterone and thyroid-stimulating hormone were associated with sperm concentration. Prolactin and anti-Mullerian hormone levels significantly correlated (p ≤ 0.01) with sperm concentration and DFI value simultaneously.
Conclusion: Decreased and increased levels of serum hormones could adversely affect semen profile and sperm DNA integrity which lead to severe male infertility. Although we investigated the effects of the main hormones related to male infertility on DNA damage, the role of these hormones on the fertilization rate and embryo quality needs to be evaluated in further studies.
Objective: This study aimed to evaluate the effects of hormonal changes including prolactin, TSH, testosterone, luteinizing hormone, follicle-stimulating hormone and anti-Mullerian hormone on sperm DNA fragmentation in normal men compared with OAT to design a clinical algorithm for the comprehensive study of male factor infertilities.
Materials and Methods: We consecutively selected 60 candidates referred to the infertility clinic to collect the semen and blood samples. Then, a terminal deoxynucleotidyl transferase dUTP nick end labeling test was performed to evaluate the sperm DNA fragmentation index (DFI). After semen analysis and DFI checking, they were classified into 4 groups consisting of normospermia and OAT men each with or without increased DFI. Hormone parameters were analyzed using enzyme-linked immunoassay.
Results: Follicle-stimulating hormone and luteinizing hormone levels showed positive correlations with DFI in a significant way (p ≤ 0.01), while testosterone and thyroid-stimulating hormone were associated with sperm concentration. Prolactin and anti-Mullerian hormone levels significantly correlated (p ≤ 0.01) with sperm concentration and DFI value simultaneously.
Conclusion: Decreased and increased levels of serum hormones could adversely affect semen profile and sperm DNA integrity which lead to severe male infertility. Although we investigated the effects of the main hormones related to male infertility on DNA damage, the role of these hormones on the fertilization rate and embryo quality needs to be evaluated in further studies.
Type of Study: Original Article |
Subject:
Reproductive Andrology
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