Volume 9, Issue 3 (7-2011)                   IJRM 2011, 9(3): 209-216 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Nazari S, Khalili M A, Esmaielzadeh F, Mohsenzadeh M. Maturation capacity, morphology and morphometric assessment of human immature oocytes after vitrification and in-vitro maturation. IJRM 2011; 9 (3) :209-216
URL: http://ijrm.ir/article-1-229-en.html
Maturation capacity, morphology and morphometric assessment of human immature oocytes after vitrification and in-vitro maturation
Saeedeh Nazari * 1, Mohammad Ali Khalili1 , Forouzan Esmaielzadeh2 , Mehdi Mohsenzadeh3
1- Researh and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Department of Animal Sciences, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran
3- Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:   (3079 Views)
Background: In general, 15% of oocytes collected in ART cycles are immature. These oocytes may be cryopreserved further for use in in-vitro maturation (IVM) program.
Objective: The aim of this study was to determine maturation capacity, morphometric parameters and morphology of human immature oocytes in both fresh IVM (fIVM) and vitrified-IVM (vIVM) oocytes.
Materials and Methods: 93 women who underwent controlled ovarian stimulation for ART were included. The immature oocytes (n=203) were divided into two groups: the first group (n=101) directly matured in vitro; and the second group (n=102) first vitrified, then matured in vitro. All oocytes underwent IVM in Ham’s F10 supplemented with LH+FSH and human follicular fluid. After 48h of incubation, the oocyte maturation rates, as well as morphometric and morphologic characteristics were assessed using cornus imaging and were compared.
Results: Oocyte maturation rates were reduced in vIVM, (40.4%), in comparison with fIVM (59.4%, p<0.001). Following morphometric assessment, there was no difference in the mean oocyte diameters (µm) between fIVM and vIVM, 156.3±6.8 and 154.07±9.9, respectively. Other parameters of perimeters, egg areas, as well as oocyte and ooplasm volumes were similar in two groups. In addition, more morphologic abnormalities, such as, vacuole, and dark oocyte were observed in vIVM oocytes.
Conclusion: fIVM was more successful than vIVM groups. No statistical differences were noticed in morphometry assessment in two groups. This suggests that morphometric parameters can not be applied as prognosis factor in oocyte maturation outcome in IVM program.
Keywords: IVM, Oocyte, Vitrification, Maturation, Morphology, Morphometry.
Full-Text [PDF 443 kb]   (631 Downloads) |   |   Full-Text (HTML)  (574 Views)  
Type of Study: Original Article |
References
1. Ben-Ami I, Komsky A, Bern O, kasterstein E, Komarovsky D, Ron-El R. In vitro maturation of human germinal vesicle-stage oocytes: role of epidermal growth factor-like growth factors in the culture medium. Hum Reprod 2011; 26: 76-78. [DOI:10.1093/humrep/deq290]
2. Liu S, Jing JJ, Feng HL, Ma SY, Li M, Li Y. Evaluation of the immature human oocytes from unstimulated cycles in polycystic ovary syndrome patients using a novel scoring system. Fertil Steril 2010; 93: 2202-2209. [DOI:10.1016/j.fertnstert.2009.01.062]
3. Al-hasani S, Ozmen B, Koutlaki N, Schoepper B, Diedrich K, Schultze-mosgau A. three years of routine vitrification of human zygotes: is it still fair to advocate slow-rate freezing? Reprod Biomed Online 2007; 14: 288-293. [DOI:10.1016/S1472-6483(10)60869-3]
4. wininger JD, Kort HI. cryopreservation of immature and mature human oocytes. Seminreprod Med 2002; 20: 45-49. [DOI:10.1055/s-2002-23519]
5. Vajta G, Nagy ZP. Are programmable freezers still needed in embryo laboratory? Reprod Biomed Online 2006; 12: 779-796. [DOI:10.1016/S1472-6483(10)61091-7]
6. Humburg R, Van der veen F, Silber SJ. Oocyte vitrification-women's emancipation set in stone. Fertil Steril 2009; 91: 1319-1320. [DOI:10.1016/j.fertnstert.2008.02.127]
7. Cobo A, Kuwayama M, Pérez S, Ruiz A, Pellicer A, Remohí J. Comparison of concomitant outcome achieved with fresh and cryopreserved donor oocytes vitrified by the cryotop method. Fertil Steril 2008; 89: 1657-1664. [DOI:10.1016/j.fertnstert.2007.05.050]
8. Cha KY, Koo JJ, Ko JJ, Choi DH, Han SY, Yoon TK . pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril 1991; 55: 109-113. [DOI:10.1016/S0015-0282(16)54068-0]
9. Trounson A, wood C, Kausche A. In vitro maturation and the fertilization and developmental compotence oocytes recovered from untreated polycystic ovarian patients. Fertil Steril 1994; 62: 353-362. [DOI:10.1016/S0015-0282(16)56891-5]
10. Durinzi KL, Saniga EM, Lanzerdorf SE. the relationship between : size and maturation in vitro in the unstimulated human oocyte. Fertil Steril 1995; 63: 404-406. [DOI:10.1016/S0015-0282(16)57377-4]
11. Mikkelsen AL, Lindenberg S. morphology of in-vitro matured oocytes: impact on fertility potential and embryo quality. Hum Reprod 2001; 16: 1714-1718. [DOI:10.1093/humrep/16.8.1714]
12. Alberts b, Brray D, Lewis J, Raff M, Robert K, Waston JD. Molecular biology of the cell. Garland publishing Inc; 1983.
13. Lasiene K, Vitkuse A, Valanciute A, Lasys V. morphological criteria of oocyte quality. Medicina (Kaunas) 2009; 45: 509-514. [DOI:10.3390/medicina45070067]
14. Bao S, Obata Y, Carroll J, Domeki I, Kono T. epigenetic modifications neccessary for normal development are estabilished during oocyte growth in mice. Biol Reprod 2000; 62: 616-621. [DOI:10.1095/biolreprod62.3.616]
15. Cavilla JL, Kennedy CR, Byskov AG, Hatshorne GM. human immature oocytes grow during cultue for IVM. Hum Reprod 2008; 23: 37-45. [DOI:10.1093/humrep/dem178]
16. Michelmann HW, Bogdan A, Hinney B. micromorphometry and spermatozoa binding patterns of fertilized and unfertilized human oocytes after in-vitro fertilization. Hum Reprod 1995; 10: 3154-3160. [DOI:10.1093/oxfordjournals.humrep.a135878]
17. kim BK, Lee SC, Kim KJ, Han CH, Kim JH. in vitro maturation, fertilization and development of human germinal vesicle oocytes collected from stimulated cycles. Fertil Steril 2000; 74: 1153-1158. [DOI:10.1016/S0015-0282(00)01617-4]
18. Cao YX, Chian RC. fertility preservation with immature and in vitro matured oocytes. Semin Reprod Med 2009; 27: 457-464. [DOI:10.1055/s-0029-1241055]
19. Paynter SJ. cryopreservation of human unfertilized oocytes. Hum Reprod Update 2000; 6: 449-456. [DOI:10.1093/humupd/6.5.449]
20. Russell JB. Immature oocyte retrieval combined with in-vitro oocyte maturation. Hum Reprod 1998; 13: 63-70. [DOI:10.1093/humrep/13.suppl_3.63]
21. Toth TH, Baka SG, Veeck LL, Jones HW, Muasher S, Lanzendorf SE. fertilization and in vitro development of cryopreserved human prophase 1 oocytes. Fertil Steril 1994; 61: 891-894. [DOI:10.1016/S0015-0282(16)56702-8]
22. Gougeon A. Dynamics of follicular growth in the human: a model from preliminary results. Hum Reprod 1986; 1: 81-87. [DOI:10.1093/oxfordjournals.humrep.a136365]
23. veek L. abnormal morphology of the human oocyte and conceptus. In: An Atlas of Human Gametes and Conceptuses. Lancs; 1999.
24. Bertand E, Van den Bergh M, Englert Y. Does zona pellucida tickness influence the fertilization rate? Hum Reprod 1995; 10: 1189-1193. [DOI:10.1093/oxfordjournals.humrep.a136116]
25. Yuan YQ, Van Soom A, Leroy JL, Dewulf J, Van Zeveren A, de Kruif A, et al. Apoptosis in cumulus cells , but not in oocytes may influence bovine embryopnic developmental compotence. Theriogenology 2005; 63: 2147-2163. [DOI:10.1016/j.theriogenology.2004.09.054]
26. Zeuner A, Muller K, Reguszynski K, Jewgenow K. apoptosis within bovine follicular cells and its effect on oocyte development during in viro maturation. Theriogenology 2003; 59: 1421-1433. [DOI:10.1016/S0093-691X(02)01190-1]
27. Balaban B, Urman B. Effect of oocyte morphology on embryo development and implantation. Reprod Biomed Online 2006; 12: 608-615. [DOI:10.1016/S1472-6483(10)61187-X]
28. Xia P. Intracytoplasmic sperm injection: correlation of oocyte grade based on polar body, perivitelline space and cytoplasmic inclusions with fertilization rate and embryo quality. Hum Reprod 1997; 12: 1750-1755. [DOI:10.1093/humrep/12.8.1750]
29. Ubaldi F, Rienzi L. morphological selection of gamets. Placenta 2008; 29: 5115-5120. [DOI:10.1016/j.placenta.2008.08.009]
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb