Volume 3, Issue 1 (7-2005)                   IJRM 2005, 3(1): 30-35 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Arefi S, Jeddi Tehrani M, Akhondi M M, Mousavi A R, Heidari M, Bayat A A et al . Anti-zona pellucida antibodies in infertile patients in relation to multiple puncture of ovaries and unexplained infertility. IJRM. 2005; 3 (1) :30-35
URL: http://ijrm.ssu.ac.ir/article-1-29-en.html
Abstract:   (1936 Views)
Background: Auto antibodies to zona-pellucida (AZA) seem to be important autoantibodies implicated in reproduction, with substantial role in both endocrine and reproductive functions of the human ovary. There are some debates on the relation of AZA with infertility, repeated In Vitro Fertilization (IVF) attempts, and outcome of it. Objective: In this study, we assessed the presence of AZA in the follicular fluids (FFs) of women who underwent intra cytoplasmic sperm injection (ICSI), in relation to etiology of infertility and multiple puncture of ovaries. Materials and Methods: In this prospective study, follicular fluids were evaluated from 96 infertile women, (19-40 years old, 31.5�5.1), who were candidates for ICSI based on the etiology of infertility. From these 80 women had explained infertility whereas 16 had unexplained infertility. All FFs were evaluated for presence of AZA by ELISA test. Results: Twenty patients (20.8%) were positive for AZA in follicular fluid. In patients with unexplained infertility, AZA antibody in follicular fluid, was significantly higher than the group with proven etiology of infertility (p=0.001). In addition, 20.4 % of patients who had been punctured previously showed AZA in their FFs which is statistically similar to the patients who were punctured for the first time. Conclusions: The high incidence of AZA in infertile women, especially women with unexplained infertility has to be considered. Relation of the presence AZA and repeated puncture of ovaries is still debatable. Determinations of AZA are highly recommended in evaluation of infertile couples especially in patient with unexplained infertility
Full-Text [PDF 44 kb]   (303 Downloads) |   |   Full-Text (HTML)  (120 Views)  
Type of Study: Original Article |

1. Karsten U, and Donat H. Detection and significance of serum zona pellucida antibodies in sterile females. Zentralbl Gynakol 1986; 108(5): 297-304.
2. Singh J, and Mhaskar AM. Enzyme-linked immunosorbent determination of autoantibodies to zona pellucida as a possible cause of infertility in women. J Immunol Methods 1985; 79(1): 133-141. [DOI:10.1016/0022-1759(85)90399-0]
3. Kamada M, Daitoh T, Mori K, Maeda N, Hirano K, Irahara M, et al. Etiological implication of autoantibodies to zona pellucida in human female infertility. Am J Reprod Immunol 1992; 28(2): 104-109. [DOI:10.1111/j.1600-0897.1992.tb00768.x] [PMID]
4. Mikulikova L, Veselsky L, Cerny V, Martinek J, Malbohan I, Fialova L. Immunofluorescence detection of porcine anti-zona pellucida antibodies in sera of infertile women. Acta Univ Carol (Med) (Praha) 1989; 35(1-2): 63-68
5. Caudle MR, shivers CA, Wild RA. Clinical significance of naturally occurring anti-zona pellucida antibodies in infertile women. Am J Reprod Immunol Microbiol 1987; 15(4): 119-121.
6. Shivers CA, Dunbar BS.Autoantibodies to zona pellucida: a possible cause for infertility in women. Science 1977; 197(4308): 1082-1084. [DOI:10.1126/science.70076] [PMID]
7. Mori T, Nishimoto T, Kitagawa M, Noda Y, Nishimura T, Oikawa T. Possible presence of autoantibodies to zone pellucida in infertile women. Experientia 1978; 34(6): 797-799. [DOI:10.1007/BF01947334] [PMID]
8. Ulcova-Gallova Z, Mardesic T. Does in vitro fertilization (IVF) influences the levels of sperm and zona pellucida (ZP) antibodies in infertile women? Am J Reprod Immunol 1996; 36(4): 216-219. [DOI:10.1111/j.1600-0897.1996.tb00166.x] [PMID]
9. Luborsky J, Llanes B, Davies S, Binor Z, Radwanska E, Pong R. Ovarian autoimmunity: greater frequency of autoantibodies in premature menopause and unexplained infertility than in the general population. Clin Immunol 1999; 90(3): 368-374. [DOI:10.1006/clim.1998.4661] [PMID]
10. Koyama K, Hasegawa A, Tsuji Y, Isojima S. Production and characterization of monoclonal antibodies to cross-reactive antigens of human and porcine zonae pellucidae. J Reprod Immunol 1985; 7(3): 187-198. [DOI:10.1016/0165-0378(85)90050-6]
11. Dunbar BS, Avery S, Lee V, Prasad S, Schwahn D, Schwoebel E, et al. The mammalian zona pellucida: its biochemistry, immunochemistry, molecular biology, and developmental expression. Reprod Fertil Dev 1994; 6(3): 331-347. [DOI:10.1071/RD9940331] [PMID]
12. Carino C, Prasad S, Skinner S, Dunbar B, Chirinos M, Schwoebel E, et al. Localization of species conserved zona pellucida antigens in mammalian ovaries. Reprod Biomed Online 2002; 4(2): 116-126. [DOI:10.1016/S1472-6483(10)61928-1]
13. Hartmann JF, Gwatkin RB. Alteration of sites on the mammalian sperm surface following capacitation. Nature 1971; 234: 479-481. [DOI:10.1038/234479b0] [PMID]
14. Shabanowitz RB, O'Rand MG. Characterization of the human zona pellucida from fertilized and unfertilized eggs. J Reprod Fertil 1988; 82: 151-161. [DOI:10.1530/jrf.0.0820151] [PMID]
15. Papale ML, Grillo A, Leonardi E, Giuffrida G, Palumbo M, Palumbo G. Assessment of the relevance of zona pellucida antibodies in follicular fluid of in-vitro fertilization (IVF) patients. Hum Reprod 1994; 9(10): 1827-1831. [DOI:10.1093/oxfordjournals.humrep.a138342] [PMID]
16. Mantzavinos T, Dalamanga N, Hassiakos D, Dimitriadou F, Konidaris S, Zourlas PA. Assessment of autoantibodies to the zona pellucida in serum and follicular fluid in in-vitro fertilization patients. Clin Exp Obstet Gynecol 1993; 20(2): 111-115.
17. Ivanova M, Djarkova T, Mollova M, Petrov M, Tikhomirova T, Dakhno F. Zona pellucida autoantibodies in women undergoing ART. Folia Biol (Praha) 1999; 45(2): 59-62.
18. Nishimoto T, Mori T, Yamada I, Nishimura T. Autoantibodies to zona pellucida in infertile and aged women. Fertil Steril 1980; 34(6): 552-556. [DOI:10.1016/S0015-0282(16)45194-0]
19. Curtis P, Burford G, Amso N, Keith E, Shaw RW. Assessment of the relevance of zona pellucida antibodies in serum and cervical mucus in patients who have fertilization failure during in vitro fertilization. Fertil Steril 1991; 56(6): 1124-1127. [DOI:10.1016/S0015-0282(16)54727-X]
20. Barbarino-Monnier P, Jouan C, Dubois M, Gobert B, Faure G, Bene MC Anti-ovarian antibodies and in vitro fertilization: cause or consequence? Gynecol Obstet Fertil 2003; 31(9): 770-773. [DOI:10.1016/S1297-9589(03)00209-1]
21. Barbarino-Monnier P, Gobert B, Guillet-Rosso F, Bene MC, Landes P, Faure G. Antiovary antibodies, repeated attempts, and outcome of in vitro fertilization. Fertil Steril 1991; 56(5): 928-932. [DOI:10.1016/S0015-0282(16)54667-6]
22. Geva E, Vardinon N, Lessing JB, Lerner-Geva L, Azem F, Yovel I, et al. Organ-specific autoantibodies are possible markers for reproductive failure: a prospective study in an in-vitro fertilization-embryo transfer program. Hum Reprod 1996; 11(8): 1627-1631. [DOI:10.1093/oxfordjournals.humrep.a019458] [PMID]
23. Mhaskar A, Buckshee K, Talwar GP. Autoantibodies to zona pellucida in tubectomized women. Contraception 1984; 29(1): 75-82. [DOI:10.1016/0010-7824(84)90060-X]
24. Hovav Y, Almagor M, Benbenishti D, Margalioth EJ, Kafka I, Yaffe H. Immunity to zona pellucida in women with low response to ovarian stimulation, in unexplained infertility and after multiple IVF attempts. Hum Reprod 1994; 9(4): 643-645. [DOI:10.1093/oxfordjournals.humrep.a138563] [PMID]
25. Moustafa M, Ozornek MH, Krussel JS, Cupisti S, Bodden-Heidrich R, Koldovsky U, et al. The effect of antigamete antibodies on the success of assisted reproduction. Clin Exp Obstet Gynecol 1997; 24(2): 67-69.
26. Gobert B, Barbarino-Monnier P, Guillet-May F, Bene MC, Faure GC. Anti -ovary antibodies after attempts at human in vitro fertilization induced by follicular puncture rather than hormonal stimulation. J Reprod Fertil 1992; 96(1): 213-218. [DOI:10.1530/jrf.0.0960213] [PMID]

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb