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Volume 10, Issue 5 (10-2012)
IJRM 2012, 10(5): 409-412 |
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Soltani L, Kafilzadeh F, Karami Shabankareh H. The effect of various concentrations of myo-inositol in culture medium on development of bovine embryos. IJRM 2012; 10 (5) :409-412
URL: http://ijrm.ir/article-1-315-en.html
URL: http://ijrm.ir/article-1-315-en.html
1- Department of Animal Sciences, Faculty of Agriculture, Razi University, Kermanshah, Iran
2- Department of Animal Sciences, Faculty of Agriculture, Razi University, Kermanshah, Iran , kafilzadeh@razi.ac.ir
2- Department of Animal Sciences, Faculty of Agriculture, Razi University, Kermanshah, Iran , kafilzadeh@razi.ac.ir
Abstract: (2524 Views)
Background: Progress achieved in culture media formulations have resulted led to an improvement in maintaining the mammalian embryo in culture throughout the preimplantation and pre-attachment period.
Objective: The objective of this study was to evaluate the effect of various concentrations of myo-inositol during in vitro fertilization of bovine oocytes on subsequent embryo development.
Materials and Methods: Bovine cumulus oocytes complexes (COCs) were matured in vitro at 39oC, in humidified 5% CO2 atmosphere for 22-24 h. The COCs were coincubated with epididymal spermatozoa of post mortem bulls in modified TALP medium for 22-24hr. The fertilization medium used was: 1) TALP medium without myo-inositol (control); 2) control+0.02 g/l myo-inositol; 3) control+0.03 g/l myoinositol; 4) control+0.04 g/l myo-inositol. Zygotes were cultured in vitro for 8 days when the ratios of in vitro embryo development of the hatched blastocysts were assessed and compared with the control group (p<0.05).
Results: The presence of 0.04 g/l myo-inpsitol significantly improved overall morula and blastocyst rates (46.94%) compared to control (32.19%), but there was no difference in the percentage of embryos successfully developed to the morula and blastocyst stage when different levels of myo-inositol were used (46.94, 36.36 and 37.33% respectively). The mean percentage of cleavage rate was not significantly affected by treatments.
Conclusion: These results suggest that, addition of 0.04 g/l myo-inositol in TALP medium is more beneficial for subsequent bovine embryonic development.
Objective: The objective of this study was to evaluate the effect of various concentrations of myo-inositol during in vitro fertilization of bovine oocytes on subsequent embryo development.
Materials and Methods: Bovine cumulus oocytes complexes (COCs) were matured in vitro at 39oC, in humidified 5% CO2 atmosphere for 22-24 h. The COCs were coincubated with epididymal spermatozoa of post mortem bulls in modified TALP medium for 22-24hr. The fertilization medium used was: 1) TALP medium without myo-inositol (control); 2) control+0.02 g/l myo-inositol; 3) control+0.03 g/l myoinositol; 4) control+0.04 g/l myo-inositol. Zygotes were cultured in vitro for 8 days when the ratios of in vitro embryo development of the hatched blastocysts were assessed and compared with the control group (p<0.05).
Results: The presence of 0.04 g/l myo-inpsitol significantly improved overall morula and blastocyst rates (46.94%) compared to control (32.19%), but there was no difference in the percentage of embryos successfully developed to the morula and blastocyst stage when different levels of myo-inositol were used (46.94, 36.36 and 37.33% respectively). The mean percentage of cleavage rate was not significantly affected by treatments.
Conclusion: These results suggest that, addition of 0.04 g/l myo-inositol in TALP medium is more beneficial for subsequent bovine embryonic development.
Type of Study: Original Article |
References
1. Michal G. Biochemical phathways. Berlin: Spektrum; 1999.
2. Downes CP. The cellular function of myo-inositol. Biochem Soc Trans 1989; 17: 259-268. [DOI:10.1042/bst0170259]
3. Kane MT, Bavister BD. Vitamin requirements for development of eight-cell hamster embryos to hatching blastocysts in vitro. Biol Reprod 1988; 39: 1137-1143. [DOI:10.1095/biolreprod39.5.1137]
4. Daniel JC Jr. Vitamins and growth factors in the nutrition of rabbit blastocysts in vitro. Growth 1967; 31: 71-77.
5. Fahy MM, Kane MT. Incorporation of [3H] inositol in to phosphoinositides and inositol phosphates by rabbit blastocysts. Mol Reprod Dev 1993; 34: 391-395. [DOI:10.1002/mrd.1080340407]
6. Holm P, Booth PJ, Schmidt MH, Greve T, Callesen H. High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins. Theriogenology 1999; 52: 683-700. [DOI:10.1016/S0093-691X(99)00162-4]
7. Hynes AC, Sreenan JM, Kane MT. Uptake and incorporation of myo-inositol by bovine preimplantation embryos from two-cell to early blastocyst stages. Mol Reprod Dev 2000; 55: 265-269.
https://doi.org/10.1002/(SICI)1098-2795(200003)55:3<265::AID-MRD4>3.0.CO;2-6 [DOI:10.1002/(SICI)1098-2795(200003)55:33.0.CO;2-6]
8. Kafilzadeh F, Karami Shabankareh H, Soltani L, kazemine Jasemi V. Comparison of the effects of myo-inositol alone or in combination with MEM vitamins on embryo development of sanjabi sheep. J Iran Anat Sci 2011; 8: 189-196.
9. Gandhi AP, Lane M, Gardner DK, Krisher RL. A single medium supports development of bovine embryo throughout maturation, fertilization and culture. Hum Reprod 2000; 15: 395-401. [DOI:10.1093/humrep/15.2.395]
10. Parrish JJ, Susko-Parrish JL, Winer MA, First NL. Capacitation of bovine sperm by heparin. Biol Reprod 1988; 38: 1171-1180. [DOI:10.1095/biolreprod38.5.1171]
11. Eagle H, Oyama VI, Levy M, Freeman AE. Myo-Inoshol as an essential growth factor for normal and malignant human cells in tissue culture. J Biol Chem 1957; 226: 191-205.
12. Hawthorne JN. Inositol phospholipids. In: Hawthorne JN, Ansell GB. Phospholipids. Amesterdam: Elsevier Biomedical Press; 1982.
13. Vance DE. Phospholipid metabolism in eukaryotes. In: Vance DE, Vance JE, editors. Biochemistry of lipids and membranes. Menlo Park, CA: The Benjamin/ Cummings Publishing Co. Inc; 1986.
14. Kane MT. The effects of water-soluble vitamins on the expansion of rabbit blastocysts in vitro. J Exp Zool 1988; 245: 220-223. [DOI:10.1002/jez.1402450211]
15. Funahashi H, Kim NH, Stumpf TT, Cantley TC, Day BN. Presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes. Biol Reprod 1996; 54: 1412-1419. [DOI:10.1095/biolreprod54.6.1412]
16. Lim KT, Jang G, Ko KH, Lee WW, Park HJ, Kim JJ, et al. Improved in vitro bovine embryo development and increased efficiency in producing viable calves using defined media. Theriogenology 2007; 67: 293-302. [DOI:10.1016/j.theriogenology.2006.07.011]
17. Kane MT. Effects of the putative phospholipid precursors, inositol, choline, serine and ethanolamine, on formation and expansion of rabbit blastocysts in vitro. J Reprod Fertil 1989; 87: 275-279. [DOI:10.1530/jrf.0.0870275]
18. Fahy MM, Kane MT. Inositol stimulates DNA and protein synthesis, andexpansion by rabbit blastocysts in vitro. Hum Reprod 1992; 7: 550-552. [DOI:10.1093/oxfordjournals.humrep.a137688]
19. Chiu TY, Rogers MS, Briton-Jones C, Haines C. Effects of myo-inositol on the in-vitro maturation and subsequent development of mouse oocytes. Hum Reprod 2003; 18: 408-416. [DOI:10.1093/humrep/deg113]
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