Volume 10, Issue 6 (4-2012)                   IJRM 2012, 10(6): 555-560 | Back to browse issues page

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Bayat P, Darabi M R, Ghanbari A, Amiri S, Sohouli P. Effects of prenatal exposure to extremely low electro-magnetic field on in vivo derived blastocysts of mice. IJRM 2012; 10 (6) :555-560
URL: http://ijrm.ir/article-1-325-en.html
Effects of prenatal exposure to extremely low electro-magnetic field on in vivo derived blastocysts of mice
Parvin-Dokht Bayat1 , Mohmmad Reza Darabi1 , Ali Ghanbari2 , Sara Amiri * 3, Pardis Sohouli4
1- Department of Anatomy, Arak University of Medical Sciences, Arak, Iran
2- Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
3- Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran , s_amiri_glis@hotmail.com
4- Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract:   (2242 Views)
Background: Indisputable population exposure to widespread electromagnetic fields, has grown concerns over the probable health effects of these fields. Objective: The present study was aimed to examine the possible effects of 50 Hz extremely low frequency electromagnetic field (ELF-EMF) exposure on the number and quality of mice blastocysts.
Materials and Methods: In present study, 66 NMRI pregnant females divided into two treated and non-treated groups. The treated group exposed to ELF-EMF (50 Hz and 6×10-3 T). Subsequently, embryos were collected by flushing the uterine horn and Fallopian tubes on the day 3 of gestation. Number of trophoectoderm (TE) and Inner Cell Mass (ICM) cells in blastocysts were determined after differential nuclei staining using a modified method. Furthermore, number of all flushed blastocysts calculated in each group.
Results: There was no significant difference in mean number of blastocysts in treated (6.64±1.34) and none treated (8.22±1.59) groups. In treated group, there were significant decreased in total cell number of blastocysts (p=0.000), number of ICM cells (p=0.000), and number of TE cells (p=0.001) whereas the ratio of ICM/TE cells increased (p=0.002).
Conclusion: The data indicate that ELF-EMF is able to affect cellular composition of blastocysts, but it can't omit total volume of blastocysts.
Keywords: Blastocyst, Electromagnetic fields, Mice
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Type of Study: Original Article |
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