Volume 12, Issue 5 (6-2014)                   IJRM 2014, 12(5): 343-0 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mardani M, Vaez A, Razavi S. Effect of saffron on rat sperm chromatin integrity. IJRM 2014; 12 (5) :343-0
URL: http://ijrm.ir/article-1-534-en.html
Effect of saffron on rat sperm chromatin integrity
Mohammad Mardani1 , Ahmad Vaez1 , Shahnaz Razavi * 2
1- Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2- Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran , razavi@med.mui.ac.ir
Abstract:   (2797 Views)
Background: Currently, relation between reactive oxygen species (ROS) ROS concentration and semen quality was indicated. Saffron has traditionally been not only considered as a food additive but also as a medicinal herb, which has a good antioxidant properties.
Objective: The aim of this study was to evaluate the protection potency of saffron and vitamin E on sperm chromatin integrity.
Materials and Methods: Thirty adult male Wistar rats divided equally into saffron (100 mg/kg), vitamin E (100 mg/kg) and control (0.5cc distilled water /day) groups. After 60 days, cauda epididymis dissected and sperm cells were used for analysis of sperm chromatin packaging by chromomycin A3 (CMA3) staining, and sperm chromatin susceptibility to acid denaturation by acridine orange (AO) staining.
Results: The mean percentage of CMA3 positive sperm was significantly decreased in saffron and vitamin E groups relative to control group (p<0.001). Moreover, the AO staining results showed that the mean percentage of sperm with DNA damage was significantly decreased in saffron and vitamin E groups as compared with control group (p<0.001).
Conclusion: Our results purposed that saffron can protect sperm against DNA damage and chromatin anomalies.
Keywords: Antioxidants, Saffron, Vitamin E, DNA damage, Sperm chromatin.
Full-Text [PDF 203 kb]   (481 Downloads) |   |   Full-Text (HTML)  (800 Views)  
Type of Study: Original Article |
References
1. Dohle GR, Colpi GM, Hargreave TB, Papp GK, Jungwirth A, Weidner W, et al. EAU guidelines on male infertility. Eur Urol 2005; 48: 703-711. [DOI:10.1016/j.eururo.2005.06.002]
2. Geva E, Lessing JB, Lerner-Geva L, Amit A. Free radicals, antioxidants and human spermatozoa: clinical implications. Hum Reprod 1998; 13: 1422-1424. [DOI:10.1093/oxfordjournals.humrep.a019709]
3. World Health Organization. Infertility: a tabulation of available data on prevalence of primary and secondary infertility. Geneva, Switzerland: WHO Programme on Maternal and Child Health and family planning; 1991.
4. Agarwal A, Makker K, Sharma R. Clinical Relevance of Oxidative Stress in Male Factor Infertility: An Update. Am J Reprod Immunol 2008; 59: 2-11. [DOI:10.1111/j.1600-0897.2007.00559.x]
5. Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 1994; 344: 721-724. [DOI:10.1016/S0140-6736(94)92211-X]
6. Lopes S, Jurisicova A, Sun JG, Casper RF. Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa. Hum Reprod 1998; 13: 896-900. [DOI:10.1093/humrep/13.4.896]
7. Said S, Funahashi H, Niwa K. DNA stability and thioldisulphide status of rat sperm nuclei during epididymal maturation and penetration of oocytes. Zygote 1999; 7: 249-254. [DOI:10.1017/S0967199499000635]
8. Mahi CA, and Yanagimachi, R. Induction of nuclear decondensation of mammalian spermatozoa in vitro. J Reprod Fertil 1975; 44: 293-296. [DOI:10.1530/jrf.0.0440293]
9. Nasr-Esfahani MH, Salehi M, Razavi S, Anjomshoa M, Rozbahani S, Moulavi F, et al. Effect of sperm DNA damage and sperm protamine deficiency on fertilization and embryo development post-ICSI. Reprod Biomed Online 2005; 11: 198-205. [DOI:10.1016/S1472-6483(10)60959-5]
10. Agarwal A, Nallella KP, Allamaneni SS, Said TM. Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online 2004; 8: 616-627. [DOI:10.1016/S1472-6483(10)61641-0]
11. Sikka SC. Oxidative stress and role of antioxidants in normal and abnormal sperm function. Front Biosci 1996; 1: 78-86. [DOI:10.2741/A146]
12. Makker K, Agarwal A, Sharma R. Oxidative stress and male infertility. Indian J Med Res 2009; 129: 357-367.
13. Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol 2005; 3: 1-21. [DOI:10.1186/1477-7827-3-28]
14. Bansal AK, Bilaspuri GS. Antioxidant effect of vitamin E on motility, viability and lipid peroxidation of cattle spermatozoa under oxidative stress. Anim Sci Paper Report 2009; 27: 5-14.
15. Yue D, Yan L, Luo H, Xu X, Jin X. Effect of Vitamin E supplementation on semen quality and the testicular cell membranal and mitochondrial antioxidant abilities in Aohan fine-wool sheep. Anim Reprod Sci 2010; 118: 217-222. [DOI:10.1016/j.anireprosci.2009.08.004]
16. Wang S, Wang G, Barton BE, Murphy TF, Huang HF. Beneficial effects of vitamin E in sperm functions in the rat after spinal cord injury. J Androl 2007; 28: 334-341. [DOI:10.2164/jandrol.106.001164]
17. Song B, He X, Jiang H, Peng Y, Wu H, Cao Y. [Compound Xuanju Capsule combined with vitamin E improves sperm chromatin integrity]. Zhonghua Nan Ke Xue 2012; 18: 1105-1107. (In Chinese)
18. Papandreou MA, Tsachaki M, Efthimiopoulos S, Cordopatis P, Lamari FN, Margarity M. Memory enhancing effects of saffron in aged mice are correlated with antioxidant protection. Behav Brain Res 2011; 219: 197-204. [DOI:10.1016/j.bbr.2011.01.007]
19. Mansoori P, Akhondzadeh S, Raisi F, Ghaeli P, Jamshidi AH, Nasehi AA, et al. A randomized, double-blind, placebo-controlled study of safety of the adjunctive saffron on sexual dysfunction induced by a selective serotonin reuptake inhibitor. J Med Plants 2011; 10: 121-130.
20. Assimopoulou AN, Sinakos Z, Papageorgiou VP. Radical scavenging activity of Crocus sativus L. extract and its bioactive constituents. Phytother Res 2005; 19: 997-1000. [DOI:10.1002/ptr.1749]
21. Fernández JA, Pandalai S. Biology, biotechnology and biomedicine of saffron. Recent Res Dev Plant Sci 2004; 2: 127-159.
22. Premkumar K, Thirunavukkarasu C, Abraham S, Santhiya S, Ramesh A. Protective effect of saffron (Crocus sativus L.) aqueous extract against genetic damage induced by anti-tumor agents in mice. Hum Experiment Toxicol 2006; 25: 79-84. [DOI:10.1191/0960327106ht589oa]
23. Heidary M, Vahhabi S, Reza Nejadi J, Delfan B, Birjandi M, Kaviani H, et al. Effect of saffron on semen parameters of infertile men. Urology J 2008; 5: 255-259.
24. Khayatnouri M, Safavi SE, Safarmashaei S, Babazadeh D, Mikailpourardabili B. The effect of Saffron Orally Administration on Spermatogenesis Index in Rat. Adv Environ Biol 2011; 5: 1514-1521.
25. El-Demerdash FM, Yousef MI, Kedwany FS, Baghdadi HH. Cadmium-induced changes in lipid peroxidation, blood hematology, biochemical parameters and semen quality of male rats: protective role of vitamin E and b-carotene. Food Chem Toxicol 2004; 42: 1563-1571. [DOI:10.1016/j.fct.2004.05.001]
26. World Health Organization. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge university press, Cambridge; 1992.
27. Razavi S, Nasr Esfahani MH, Mardani M, Mafi A, Moghdam A. Effect of human sperm chromatin anomalies on fertilization outcome post ICSI. Andrologia 2003; 35: 238-243. [DOI:10.1046/j.1439-0272.2003.00566.x]
28. Lolis D, Georgiou I, Syrrou M, Zikopoulos K, Konstantelli M, Messinis I. Chromomycin A3-staining as an indicator of protamine deficiency and fertilization. Int J Androl 1996; 19: 23-27. [DOI:10.1111/j.1365-2605.1996.tb00429.x]
29. Nasr-Esfahani MH, Razavi S, Mardani M. Relation between different human sperm nuclear maturity tests and in vitro fertilization. J Assist Reprod Genet 2001; 18: 219-225. [DOI:10.1023/A:1009412130417]
30. Manicardi GC, Bianchi PG, Pantano S, Azzoni P, Bizzaro D, Bianchi U, et al. Presence of endogenous nicks in DNA of ejaculated human spermatozoa and its relationship to chromomycin A3 accessibility. Biol Reprod 1995; 52: 864-867. [DOI:10.1095/biolreprod52.4.864]
31. Hoshi K, Katayose H, Yanagida K, Kimura Y, Sato A. The relationship between acridine orange fluorescence of sperm nuclei and the fertilizing ability of human sperm. Fertil Steril 1996; 66: 634-639. [DOI:10.1016/S0015-0282(16)58581-1]
32. Razavi SH, Nasr-Esfahani MH, Deemeh MR, Shayesteh M, Tavalaee M. Evaluation of zeta and HA‐binding methods for selection of spermatozoa with normal morphology, protamine content and DNA integrity. Andrologia 2010; 42: 13-19. [DOI:10.1111/j.1439-0272.2009.00948.x]
33. Kemal Duru N, Morshedi M, Oehninger S. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa. Fertil Steril 2000; 74: 1200-1207. [DOI:10.1016/S0015-0282(00)01591-0]
34. Aitken RJ, Krausz C. Oxidative stress, DNA damage and the Y chromosome. Reproduction 2001; 122: 497-506. [DOI:10.1530/rep.0.1220497]
35. Suleiman SA, Ali ME, Zaki ZM, el-Malik EM, Nasr MA. Lipid peroxidation and human sperm motility: protective role of vitamin E. J Androl 1996; 17: 530.
36. Kumar V, Bhat ZA, Kumar D, Khan NA, Chashoo IA, Shah MY. Pharmacological profile of crocus sativus-a comprehe sive review. Pharmacol Online 2011; 3: 799-811.
37. Hosseinzadeh H, Ziaee T, Sadeghi A. The effect of saffron, Crocus sativus stigma, extract and its constituents, safranal and crocin on sexual behaviors in normal male rats. Phytomedicine 2008; 15: 491-495. [DOI:10.1016/j.phymed.2007.09.020]
38. Dominguez-Rebolledo AE, Fernandez-Santos MR, Bisbal A, Ros-Santaella JL, Ramon M, Carmona M, et al. Improving the effect of incubation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatments. Reprod Fertil Dev 2010; 22: 856-870. [DOI:10.1071/RD09197]
39. Safarinejad MR, Shafiei N, Safarinejad S. A prospective double-blind randomized placebo- controlled study of the effect of saffron (Crocus sativus Linn.) on semen parameters and seminal plasma antioxidant capacity in infertile men with idiopathic oligoasthenoteratozoospermia. Phytother Res 2011; 25: 508-516. [DOI:10.1002/ptr.3294]
40. Hosseinzadeh H, Abootorabi A, Sadeghnia HR. Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate-Induced DNA Damage in Mice Organs. DNA Cell Biol 2008; 27: 657-664. [DOI:10.1089/dna.2008.0767]
41. Uzunhisarcikli M, Kalender Y, Dirican K, Kalender S, Ogutcu A, Buyukkomurcu F. Acute, subacute and subchronic administration of methyl parathion-induced testicular damage in male rats and protective role of vitamins C and E. Pesticide Biochem Physiol 2007; 87: 115-122. [DOI:10.1016/j.pestbp.2006.06.010]
42. Keskes-Ammar L, Feki-Chakroun N, Rebai T, Sahnoun Z, Ghozzi H, Hammami S, et al. Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Arch Androl 2003; 49: 83-94. [DOI:10.1080/01485010390129269]
43. Paradiso Galatioto G, Gravina GL, Angelozzi G, Sacchetti A, Innominato PF, Pace G, et al. May antioxidant therapy improve sperm parameters of men with persistent oligospermia after retrograde embolization for varicocele? World J Urol 2008; 26: 97-102. [DOI:10.1007/s00345-007-0218-z]
44. Momeni HR, Soleimani Mehranjani M, Abnosi MH, Mahmoodi M. Effects of vitamin E on sperm parameters and reproductive hormones in developing rats treated with para-nonylphenol. Iran J Reprod Med 2009; 7: 111-116.
45. Kessopoulou E, Powers HJ, Sharma KK, Pearson MJ, Russell JM, Cooke ID, et al. A double-blind randomized placebo cross-over controlled trial using the antioxidant vitamin E to treat reactive oxygen species associated male infertility. Fertil Steril 1995; 64: 825. [DOI:10.1016/S0015-0282(16)57861-3]
46. Greco E, Iacobelli M, Rienzi L, Ubaldi F, Ferrero S, Tesarik J. Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment. J Androl 2005; 26: 349. [DOI:10.2164/jandrol.04146]
47. Tunc O, Thompson J, Tremellen K. Improvement in sperm DNA quality using an oral antioxidant therapy. Reprod Biomed Online 2009; 18: 761-768. [DOI:10.1016/S1472-6483(10)60024-7]
48. Menezo YJ, Hazout A, Panteix G, Robert F, Rollet J, Cohen-Bacrie P, et al. Antioxidants to reduce sperm DNA fragmentation: an unexpected adverse effect. Reprod Biomed Online 2007; 14: 418-421. [DOI:10.1016/S1472-6483(10)60887-5]
49. Piomboni P, Gambera L, Serafini F, Campanella G, Morgante G, De Leo V. Sperm quality improvement after natural anti-oxidant treatment of asthenoteratospermic men with leukocytospermia. Asian J Androl 2008; 10: 201-206. [DOI:10.1111/j.1745-7262.2008.00356.x]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb