Volume 13, Issue 2 (2-2015)                   IJRM 2015, 13(2): 85-92 | Back to browse issues page

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Taati M, Moghadasi M, Dezfoulian O, Asadian P, Zendehdel M. Effects of Ghrelin on germ cell apoptosis and proinflammatory cytokines production in Ischemia-reperfusion of the rat testis. IJRM 2015; 13 (2) :85-92
URL: http://ijrm.ir/article-1-624-en.html
Effects of Ghrelin on germ cell apoptosis and proinflammatory cytokines production in Ischemia-reperfusion of the rat testis
Majid Taati * 1, Mehrnoush Moghadasi2 , Omid Dezfoulian3 , Payman Asadian3 , Morteza Zendehdel4
1- Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran , taatimajid@yahoo.com
2- Department of Physiology, Faculty of Medicine, Lorestan University of Medical Sciences, Khoramabad, Iran
3- Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran
4- Department of Physiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Abstract:   (2607 Views)
Background: Testicular torsion is a medical emergency that requires surgical intervention to reperfuse the affected testis. Ischemia reperfusion injury is usually associated with proinflammatory cytokine generation and apoptosis of germ cells in the testes.
Objective: In this study we investigate the effect of ghrelin on the proinflammatory cytokines levels and germ cell apoptosis in testicular ischemia reperfusion.
Materials and Methods: 45 male rats were selected for the study and randomly divided into 3 groups, each containing 15 rats. Animals in the testicular torsion and ghrelin treated groups were subjected to unilateral 720 counterclockwise testicular torsion for 1 hr and then reperfusion was allowed after detorsion for 4 hr, 1 and 7 days. The ghrelin-treated group received intraperitoneal injection of ghrelin 15min before detorsion. The expression levels of bcl-2-associated X protein and proliferating cell nuclear antigen in testicular tissue in the different groups were detected by immunohistochemical assay and tissue cytokines interleukin-1β, tumor necroses factor-α and interleukin-6 were measured using enzyme-linked immunosorbent assay
Results: After being treated by ghrelin, the population of immunoreactive cells against BAX in the spermatocytes on day 7 after reperfusion significantly decreased when compared to tortion/ detortion-saline animals (p=0.024). In contrast, PCNA expression in the spermatocytes and spermatogonia were not significantly different between tortion/ detortion-ghrelin and tortion/ detortion-saline groups on both experimental days. Administration of ghrelin significantly attenuated the testicular tumor necroses factor-α and interleukin-6 levels compared with the untreated animals, but had no significant effect on the level of interleukin-1β.
Conclusion: Ghrelin offers remarkable anti-inflammatory and anti-apoptotic effects in testicular ischemia reperfusion injury.
Keywords: Ghrelin, Ischemia, Reperfusion, Testes, Apoptosis.
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Type of Study: Original Article |
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