Volume 14, Issue 2 (2-2016)                   IJRM 2016, 14(2): 125-132 | Back to browse issues page

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Khaki A A, Khaki A, Ahmadi S S. The effect of Non- ionizing electromagnetic field with a frequency of 50 Hz in Rat ovary: A transmission electron microscopy study. IJRM 2016; 14 (2) :125-132
URL: http://ijrm.ir/article-1-720-en.html
The effect of Non- ionizing electromagnetic field with a frequency of 50 Hz in Rat ovary: A transmission electron microscopy study
Amir Afshin Khaki1 , Arash Khaki2 , Seyed Shahin Ahmadi * 3
1- Tabriz Health Services Management Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2- Department of Pathobiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3- Tabriz Health Services Management Research Center, Tabriz University of Medical Sciences, Tabriz, Iran , arashkhaki@yahoo.com
Abstract:   (2997 Views)
Background: Recently, there are increasing concerns and interests about the potential effects of Electromagnetic Field (EMF) on both human and animal health.
Objective: The goal of this study was to evaluate the harmful effects of 50 Hz non-ionizing EMF on rat oocytes.
Materials and Methods: In this experimental study 30 rats were randomly taken from laboratory animals and their ags and weights were determined. These 3 month's old rats were randomly divided into 3 groups. The control group consisted of 10 rats without receiving any treatment and kept under normal conditions. Experimental group 1 (10 rats) received EMF for 8 weeks (3 weeks intrauterine +5 weeks after births) and experimental group 2 (10 rats) received EMF for 13 weeks (3 weeks intrauterine +10 weeks after birth). After removing the ovaries and isolating follicles, granulosa cells were fixed in glutaraldehyde and osmium tetroxide. Electron microscopy was used to investigate the traumatic effects of EMF on follicles.
Results: In control group nucleus membrane and mitochondria in follicle’s cytoplasm seemed normal in appearance. Theca layer of primary follicles in experimental group was separated clearly, zona layer demonstrated trot with irregular thickness and ovarian stroma seemed isolated with dilated vessels showing infiltration.
Conclusion: According to the results of this study, it can be concluded that EMF has harmful effects on the ovarian follicles
Keywords: Electromagnetic field, Ovary, Organelles, Rat
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Type of Study: Original Article |
References
1. Hardell L, Sage C. Biological effects from electromagnetic field exposure1 and public exposure standards. Biomed Pharmacother 2008; 62: 104-109. [DOI:10.1016/j.biopha.2007.12.004]
2. Carpenter DO. Human disease resulting from exposure to electromagnetic fields. Rev Environ Health 2013; 28: 159-172. [DOI:10.1515/reveh-2013-0016]
3. Cameron IL, Hardman WE, Winters WD, Zimmerman S, Zimmerman AM. Environmental magnetic fields: influences on early embryogenesis. J Cell Biochem 1993; 51: 417-425. [DOI:10.1002/jcb.2400510406]
4. Li P, McLaughlin J, Infante-Rivard C. Maternal occupational exposure to extremely low frequency magnetic fields and the risk of brain cancer in the offspring. Cancer Causes Control 2009; 20: 945-955. [DOI:10.1007/s10552-009-9311-5]
5. Infante-Rivard C, Deadman JE. Maternal occupational exposure to extremely low frequency magnetic fields during pregnancy and childhood leukemia. Epidemiology 2003; 14: 437-441. [DOI:10.1097/01.ede.0000078421.60231.bc]
6. Sambucci M, Laudisi F, Nasta F, Pinto R, Lodato R, Altavista P, et al. Prenatal exposure to non-ionizing radiation: effects of WiFi signals on pregnancy outcome, peripheral B-cell compartment and antibody production. Radiat Res 2010; 174: 732-740. [DOI:10.1667/RR2255.1]
7. Nishimura I, Oshima A, Shibuya K, Negishi T. Lack of teratological effects in rats exposed to 20 or 60 kHz magnetic fields. Birth Defects Res B Dev Reprod Toxicol 2011; 92: 469-477. [DOI:10.1002/bdrb.20316]
8. Khaki AA, Zarrintan S, Khaki A, Zahedi A. The effects of electromagnetic field on the microstructure of seminal vesicles in rat: a light and transmission electron microscope study. Pak J Biol Sci 2008;11: 692-701. [DOI:10.3923/pjbs.2008.692.701]
9. Khaki AA, Tubbs RS, Shoja MM, Rad JS, Khaki A, Farahani RM, et al. The effects of an electromagnetic field on the boundary tissue of the seminiferous tubules of the rat: A light and transmission electron microscope study. Folia Morphol (Warsz) 2006; 65: 188-194.
10. Hajhosseini L, Khaki A, Merat E, Ainehchi N. Effect of rosmarinic acid on sertoli cells apoptosis and serum antioxidant levels in rats after exposure to electromagnetic fields. Afr J Tradit Complement Altern Med 2013; 10: 477-480. [DOI:10.4314/ajtcam.v10i6.14]
11. Edelstyn N, Oldershaw A. The acute effects of exposure to the electromagnetic field emitted by mobile phones on human attention. Neuroreport 2002; 13: 119-121. [DOI:10.1097/00001756-200201210-00028]
12. Kaprana AE, Karatzanis AD, Prokopakis EP, Panagiotaki IE, Vardiambasis IO, Adamidis G, et al. Studying the effects of mobile phone use on the auditory system and the central nervous system: a review of the literature and future directions. Eur Arch Otorhinolaryngol 2008 265: 1011-1019. [DOI:10.1007/s00405-008-0703-z]
13. Cao YN, Zhang Y, Liu Y. Effects of exposure to extremely low frequency electromagnetic fields on reproduction of female mice and development of offsprings. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2006; 24: 468-470.
14. Odaci E, Bas O, Kaplan S. Effects of prenatal exposure to a 900 MHz electromagnetic field on the dentate gyrus of rats: a stereological and histopathological study. Brain Res 2008; 31: 224-229. [DOI:10.1016/j.brainres.2008.08.013]
15. Han J, Cao Z, Liu X, Zhang W, Zhang S. Effect of early pregnancy electromagnetic field exposure on embryo growth ceasing. Wei Sheng Yan Jiu 2010; 39: 349-352.
16. Huuskonen H, Juutilainen J, Komulainen H. Effects of low-frequency magnetic fields on fetal development in rats. Bioelectromagnetics 1993; 14: 205-213. [DOI:10.1002/bem.2250140304]
17. Li DK, Chen H, Odouli R. Maternal exposure to magnetic fields during pregnancy in relation to the risk of asthma in offspring. Arch Pediatr Adolesc Med 2011; 165: 945-950. [DOI:10.1001/archpediatrics.2011.135]
18. Chung MK, Lee SJ, Kim YB, Park SC, Shin DH, Kim SH, et al. Evaluation of spermatogenesis and fertility in F1 male rats after in utero and neonatal exposure to extremely low frequency electromagnetic fields. Asian J Androl 2005; 7: 189-194. [DOI:10.1111/j.1745-7262.2005.00007.x]
19. Wu YL, Ma SR, Peng T, Teng ZH, Liang XY, Guo GZ, et al. Effects of pulsed electromagnetic field on differentiation of HUES-17 human embryonic stem cell line. Int J Mol Sci 2014; 15: 14180-14190. [DOI:10.3390/ijms150814180]
20. Nicholas CR, Haston KM, Pera RA. Intact fetal ovarian cord formation promotes mouse oocyte survival and development. BMC Dev Biol 2010, 10: 2. [DOI:10.1186/1471-213X-10-2]
21. Farsi A, Khaki A, Fathiazad F, Afshari F, Hajhosseini L, Khaki AA. Improvement effect of rosmarinic acid on serum testosterone level after exposing with electromagnetic. International Journal of Women's Health and Reproduction Sciences 2013; 1: 45-50. [DOI:10.15296/ijwhr.2013.08]
22. Devine PJ, Payne CM, McCuskey MK, Hoyer PB. Ultrastructural evaluation of oocytes during atresia in rat ovarian follicles. Biol Reprod 2000; 63: 1245-1252. [DOI:10.1095/biolreprod63.5.1245]
23. Cecconi S, Gualtieri G, Di Bartolomeo A, Troiani G, Cifone MG, Canipari R. Evaluation of the effects of extremely low frequency electromagnetic fields on mammalian follicle development. Hum Reprod 2000; 15: 2319-2325. [DOI:10.1093/humrep/15.11.2319]
24. Bakacak M, Bostancı MS, Attar R, Yıldırım ÖK, Yıldırım G, Bakacak Z, et al. The effects of electromagnetic fields on the number of ovarian primordial follicles: An experimental study. Kaohsiung J Med Sci 2015; 31: 287-292. [DOI:10.1016/j.kjms.2015.03.004]
25. Cheronff N, Rogers JM, Kavet R. A review of the literature on potential reproductive and developpmental toxicity of electric and magnetic fields. Toxicology 1992; 74: 91-126 [DOI:10.1016/0300-483X(92)90132-X]
26. Khaki A, Khaki AA, Zahedi Afshin. Effect of Ocimum basilicum on Ovary tissue Apoptosis after exposed with extremely low frequency electromagnetic fields (ELF-EMF) in Rats. Life Sci J 2013; 10: 317-321.
27. Khaki A, Khaki AA. Recovery Effect of Basil on ovarian tissue artery Hyperemia after exposed with electromagnetic field. Health MED 2013; 8: p2375.
28. Roshangar L, Hamdi BA, Khaki AA, Soleimani-Rad J, Soleimani-Rad S. Effect of low-frequency electromagnetic field exposure on oocyte differentiation and follicular development. Adv Biomed Res 2014; 3: 76. [DOI:10.4103/2277-9175.125874]
29. Belyaev I, Dean A, Eger H, Hubmann G, Jandrisovits R, Johansson O, et al. EUROPAEM EMF Guideline 2015 for the prevention, diagnosis and treatment of EMF-related health problems and illnesses. 2015; 30: 337-371.
30. Panagopoulos DJ, Johansson O, Carlo GL. Real versus Simulated Mobile Phone Exposures in Experimental Studies. Biomed Res Int 2015; 2015: 607053-607061. [DOI:10.1155/2015/607053]
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