Volume 14, Issue 3 (3-2016)                   IJRM 2016, 14(3): 173-180 | Back to browse issues page

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Asadzadeh R, Khosravi S, Zavareh S, Ghorbanian M T, Paylakhi S H, Mohebbi S R. Vitrification affects the expression of matrix metalloproteinases and their tissue inhibitors of mouse ovarian tissue. IJRM 2016; 14 (3) :173-180
URL: http://ijrm.ir/article-1-735-en.html
Vitrification affects the expression of matrix metalloproteinases and their tissue inhibitors of mouse ovarian tissue
Reza Asadzadeh1 , Shima Khosravi1 , Saeed Zavareh * 2, Mohammad Taghi Ghorbanian1 , Seyed Hassan Paylakhi1 , Seyed Reza Mohebbi1
1- School of Biology, Damghan University, Damghan, Iran
2- School of Biology, Damghan University, Damghan, Iran , Zavareh.S@du.ac.ir
Abstract:   (2674 Views)
Background: One of the most major obstacles of ovarian tissue vitrification is suboptimal developmental competence of follicles. Matrix metalloproteinases 2 (MMP-2) and 9 (MMP-9) and their tissue inhibitors TIMP-1 and TIMP-2 are involved in the remodeling of the extracellular matrix in the ovaries.
Objective: This study aimed to evaluate the expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 genes in the preantral follicles derived from vitrified mouse ovaries.
Materials and Methods: In this experimental study, the gene expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the isolated preantral follicles derived from fresh and vitrified ovaries of 14-16 days old female mice through real time qRT-PCR was evaluated. Developmental parameters, including survival rate, growth, antrum formation and metaphase II oocytes were also analyzed.
Results: The developmental parameters of fresh preantral follicles were significantly higher than vitrified preantral follicles. The TIMP-1 and MMP-9 expression levels showed no differences between fresh and vitrified preantral follicles (p=0.22, p=0.11 respectively). By contrast, TIMP-2 expression significantly decreased (p=0.00) and MMP-2 expression increased significantly (p=0.00) in vitrified preantral follicles compared with to fresh ones.
Conclusion: Changes in expression of MMP-2 and TIMP-2 after ovarian tissues vitrification is partially correlated with decrease in follicle development.
Keywords: Vitrification, Pre antral follicles, Matrix metalloproteinases, Mouse
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Type of Study: Original Article |
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