Volume 14, Issue 6 (6-2016)                   IJRM 2016, 14(6): 383-388 | Back to browse issues page

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Heydarian N, Favaedi R, Sadighi Gilani M A, Shahhoseini M. Expression level of chromodomain Y (CDY): potential marker for prediction of sperm recovery in non-obstructive azoospermia. IJRM 2016; 14 (6) :383-388
URL: http://ijrm.ir/article-1-760-en.html
Expression level of chromodomain Y (CDY): potential marker for prediction of sperm recovery in non-obstructive azoospermia
Neda Heydarian1 , Raha Favaedi2 , Mohammad Ali Sadighi Gilani3 , Maryam Shahhoseini * 2
1- Department of Biology, Faculty of Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2- Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
3- Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Abstract:   (2712 Views)
Background: The availability of testis specific genes will be of help in choosing the most promising biomarkers for the detection of testicular sperm retrieval in patients with non-obstructive azoospermia (NOA). Testis specific chromodomain protein Y 1 (CDY1) is a histone acetyltransferase which concentrates in the round spermatid nucleus, where histone hyperacetylation occurs and causes the replacement of histones by the sperm-specific DNA packaging proteins, TNPs and PRMs.
Objective: The aim was to evaluate CDY1 gene as a marker for predicting of successful sperm retrieval in NOA patients.
Materials and Methods: This research was conducted on 29 patients with NOA who had undergone testicular sperm extraction (TESE) procedure. NOA patients were subdivided into patients with successful sperm retrieval (NOA+, n=12) and patients with unsuccessful sperm retrieval (NOA-, n=17). Relative expression of CDY1 gene and chromatin incorporation of CDY1 protein were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and ELISA assay, respectively.
Results: Quantification of mRNA relative expression and incorporation of CDY1 protein in chromatin showed significant lower expressions and protein levels of CDY1 in testis tissues of NOA- in comparison to NOA+ group.
Conclusion: The findings in this study demonstrated a correlation between the low levels of CDY1 function and unsuccessful sperm recovery in the testicular tissues of NOA- compared to NOA+ patients. Therefore, it can be reasonable to consider CDY1 as a potential biomarker for predicting the presence of spermatozoa, although the claim needs more samples to be confirmed.
Keywords: Azoospermia, CDY1, Gene expression, Marker
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Type of Study: Original Article |
References
1. Ishikawa T. Surgical recovery of sperm in non-obstructive Azoospermia. Asian J Androl 2012; 14: 109-115. [DOI:10.1038/aja.2011.61]
2. Esteves SC, Agarwa A. Sperm retrieval rates and intracytoplasmic sperm injection outcomes for men with non-obstructive azoospermia and the health of resulting offspring. Asian J Androl 2014; 16: 642. [DOI:10.4103/1008-682X.127817]
3. Plas E, Riedl CR, Engelhardt PF, Muhlbauer H, Pfluger H. Unilateral or bilateral testicular biopsy in the era of intracytoplasmic sperm injection. J Urol 1999; 162: 2010-2013. [DOI:10.1016/S0022-5347(05)68089-5]
4. Tsujimura A, Matsumiya K, Miyagawa Y, 59TTakao T59T, 59TFujita K59T, 59TKoga M59T, et al. Prediction of successful outcome of microdissection testicular sperm extraction in men with idiopathic nonobstructive azoospermia. J Urol 2004; 172: 1944-1997. [DOI:10.1097/01.ju.0000142885.20116.60]
5. Bonaparte E, Moretti M, Colpi GM, Nerva F, Contalbi G, Vaccalluzzo L, et al. ESX1 gene expression as a robust marker of residual spermatogenesis in azoospermic men. Hum Reprod 2010; 25: 1398-1403. [DOI:10.1093/humrep/deq074]
6. Kleiman SE, Lagziel A, Yogev L, Botchan A, Paz G, Yavetz H. Expression of CDY1 may identify complete spermatogenesis. Fertil Steril 2001; 75: 166-173. [DOI:10.1016/S0015-0282(00)01639-3]
7. Haraguchi T, Ishikawa T, Yamaguchi K, Fujisawa M. Cyclin and protamine as prognostic molecular marker for testicular sperm extraction in patients with azoospermia. Fertil Steril 2009; 91: 1424-1426. [DOI:10.1016/j.fertnstert.2008.05.072]
8. Zamudio NM, Chong S, O'Bryan MK. Epigenetic regulation in male germ cells. Reproduction 2008; 136: 131-146. [DOI:10.1530/REP-07-0576]
9. Khalil AM, Wahlestedt C. Epigenetic mechanisms of gene regulation during mammalian spermatogenesis. Epigenetics 2008; 3: 21-27. [DOI:10.4161/epi.3.1.5555]
10. Oakberg EF. A description of spermiogenesis in the mouse and its use in analysis of the cycle of the seminiferous epithelium and germ cell renewal. Am J Anat 1956; 99: 391-413. [DOI:10.1002/aja.1000990303]
11. Lahn BT, Page D. Retroposition of autosomal mRNA yielded testis-specific gene family on human Y chromosome. Nat Genet 1999; 21: 429-433. [DOI:10.1038/7771]
12. Kleiman SE, Yogev L, Hauser R, Botchan A, Bar-Shira Maymon B, Schreiber L, et al. Members of the CDY family have different expression patterns: CDY1transcripts have the best correlation with complete spermatogenesis. Hum Genet 2003; 113: 486-492. [DOI:10.1007/s00439-003-0990-9]
13. Stahl PJ, Mielnik AN, Barbieri CE, Schlegel PN, Paduch DA. Deletion or under expression of the Y-chromosome genes CDY2 and HSFY is associated with maturation arrest in American men with nonobstructive azoospermia. Asian J Androl 2012; 14: 676-682. [DOI:10.1038/aja.2012.55]
14. Kleiman SE, Lehavi O, 59THauser R59T, 59TBotchan A59T, 59TPaz G59T, 59TYavetz H59T, et al. CDY1 and BOULE transcripts assessed in the same biopsy as predictive markers for successful testicular sperm retrieval. Fertil Steril 2011; 95: 2297-2302. [DOI:10.1016/j.fertnstert.2011.03.020]
15. Ghorbel M, Baklouti-Gargouri S, Keskes R, Chakroun N, Sellami A, Fakhfakh F, Ammar-Keskes L. Deletion of CY1b copy of Y chromosome CDY1 gene is a risk factor of male infertility in Tunisian men. Gene 2014; 548: 251-255. [DOI:10.1016/j.gene.2014.07.042]
16. Dai B, Rasmussen T P. Global epiproteomic signatures distinguish embryonic stem cells from differentiated cells. Stem Cells 2007; 25: 2567-2574. [DOI:10.1634/stemcells.2007-0131]
17. Oates R. Evaluation of the azoospermic male. Asian J Androl 2012; 14: 82-87. [DOI:10.1038/aja.2011.60]
18. Paz G, Gamzu R, Yavetz H. Diagnosis of Nonobstructive Azoospermia: The Laboratory Perspective. J Androl 2003; 24: 167-169. [DOI:10.1002/j.1939-4640.2003.tb02657.x]
19. Malcher A, Rozwadowska N, Stokowy T, Kolanowski T, Jedrzejczak P, Zietkowiak W, et al. Potential biomarkers of non-obstructive azoospermia identified in microarray gene expression analysis. Fertil Steril 2013; 100: 1686-1694. [DOI:10.1016/j.fertnstert.2013.07.1999]
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