International Journal of
Reproductive Biomedicine
Sat, Apr 20, 2024
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Volume 5, Issue 4 (7-2007)
IJRM 2007, 5(4): 103-107 |
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Ghaffari Novin M, Heidari M, Akhondi M A, Jeddi Tehrani M. In vitro application of Matrigel enhances human blastocyst formation and hatching. IJRM 2007; 5 (4) :103-107
URL: http://ijrm.ir/article-1-77-en.html
URL: http://ijrm.ir/article-1-77-en.html
1- Reproductive Biotechnology Research Centre, Avesina Research Institute, ACECR, Tehran, Iran , mghaffarin@yahoo.com
2- Nanobiotechnology Research Centre, Avesina Research Institute, ACECR, Tehran, Iran
3- Reproductive Biotechnology Research Centre, Avesina Research Institute, ACECR, Tehran, Iran
4- Monoclonal Antibody Research Center, Avesina Research Institute, ACECR, Tehran, Iran
2- Nanobiotechnology Research Centre, Avesina Research Institute, ACECR, Tehran, Iran
3- Reproductive Biotechnology Research Centre, Avesina Research Institute, ACECR, Tehran, Iran
4- Monoclonal Antibody Research Center, Avesina Research Institute, ACECR, Tehran, Iran
Abstract: (2184 Views)
Background: Matrigel (extracellular matrix) can improve the growth of many cell types in vitro.
Objective: The aim of the present study was to determine the effect of Matrigel on the development of 2-4 cells human embryos in culture.
Material and Methods: Surplus 2-4 cells human embryos, resulting from ICSI, were divided into two groups (control and test). Quality of embryos in both groups was morphologically similar. The test group (n=140) was cultured in Hams’ F10 supplemented with 10% human serum albumin and 150 µl liquid Matrigel. The control group (n=140) was cultured in the same medium devoid of Matrigel. Embryos were cultured for an additional 4 days and their morphology was assessed every 24 hours. Both groups were then statistically compared.
Results: The percentage of the human embryos that reached the morula stage in the control and test groups were 79.2% and 80%, respectively (p>0.05). However, 36.4% of embryos reached the blastocyst stage in the test group as compared to 5.7% in the control group after 144 hours in culture. This difference was statically significant (p <0.01). In addition, culture of embryos on Matrigel and medium versus medium alone significantly improved in vitro hatching (25.7% versus 3.5%; p <0.01).
Conclusion: Matrigel at low concentration enhances human blastocyst formation and hatching in vitro.
Objective: The aim of the present study was to determine the effect of Matrigel on the development of 2-4 cells human embryos in culture.
Material and Methods: Surplus 2-4 cells human embryos, resulting from ICSI, were divided into two groups (control and test). Quality of embryos in both groups was morphologically similar. The test group (n=140) was cultured in Hams’ F10 supplemented with 10% human serum albumin and 150 µl liquid Matrigel. The control group (n=140) was cultured in the same medium devoid of Matrigel. Embryos were cultured for an additional 4 days and their morphology was assessed every 24 hours. Both groups were then statistically compared.
Results: The percentage of the human embryos that reached the morula stage in the control and test groups were 79.2% and 80%, respectively (p>0.05). However, 36.4% of embryos reached the blastocyst stage in the test group as compared to 5.7% in the control group after 144 hours in culture. This difference was statically significant (p <0.01). In addition, culture of embryos on Matrigel and medium versus medium alone significantly improved in vitro hatching (25.7% versus 3.5%; p <0.01).
Conclusion: Matrigel at low concentration enhances human blastocyst formation and hatching in vitro.
Type of Study: Original Article |
References
1. Carnegie J, Claman P, Lawrence C, Cabaca O. Can matrigel substitute for Vero cells in promoting the in-vitro development of mouse embryos? Hum. Reprod 1995; 10: 636-641. [DOI:10.1093/oxfordjournals.humrep.a136002]
2. Desai N, Scarrow M, Lawson J, Kinzer D, Goldfarb J. Evaluation of the effect of interleukin-6 and human extracellullar matrix on embryonic development. Hum Reprod. 1999; 14:1588-1592. [DOI:10.1093/humrep/14.6.1588]
3. Criniti A, Thyer A, Chow G, Lin P, Klein N, Soules M. Elective single blastocyst transfer reduces twin rates without compromising pregnancy rates. Fertil Stril 2005; 84:1613-1619. [DOI:10.1016/j.fertnstert.2005.06.035]
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