International Journal of
Reproductive Biomedicine
Sat, Apr 20, 2024
[Archive]
Volume 14, Issue 7 (7-2016)
IJRM 2016, 14(7): 443-452 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Arun S, Burawat J, Sukhorum W, Sampannang A, Maneenin C, Iamsaard S. Chronic restraint stress induces sperm acrosome reaction and changes in testicular tyrosine phosphorylated proteins in rats. IJRM 2016; 14 (7) :443-452
URL: http://ijrm.ir/article-1-770-en.html
URL: http://ijrm.ir/article-1-770-en.html
Supatcharee Arun1 
, Jaturon Burawat1 , Wannisa Sukhorum1 , Apichakan Sampannang1 , Chanwit Maneenin1 , Sitthichai Iamsaard * 2
, Jaturon Burawat1 , Wannisa Sukhorum1 , Apichakan Sampannang1 , Chanwit Maneenin1 , Sitthichai Iamsaard * 2
1- Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
2- Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand , sittia@kku.ac.th
2- Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand , sittia@kku.ac.th
Abstract: (3170 Views)
Background: Stress is a cause of male infertility. Although sex hormones and sperm quality have been shown to be low in stress, sperm physiology and testicular functional proteins, such as phosphotyrosine proteins, have not been documented.
Objective: To investigate the acrosome status and alterations of testicular proteins involved in spermatogenesis and testosterone synthesis in chronic stress in rats.
Materials and Methods: In this experimental study, male rats were divided into 2 groups (control and chronic stress (CS), n=7). CS rats were immobilized (4 hr/day) for 42 consecutive days. The blood glucose level (BGL), corticosterone, testosterone, acrosome status, and histopathology were examined. The expressions of testicular steroidogenic acute regulatory (StAR), cytochrome P450 side chain cleavage (CYP11A1), and phosphorylated proteins were analyzed.
Results: Results showed that BGL (71.25±2.22 vs. 95.60±3.36 mg/dl), corticosterone level (24.33±4.23 vs. 36.9±2.01 ng/ml), acrosome reacted sperm (3.25±1.55 vs. 17.71±5.03%), and sperm head abnormality (3.29±0.71 vs. 6.21±1.18%) were significantly higher in CS group in comparison with control. In contrast, seminal vesicle (0.41±0.05 vs. 0.24±0.07 g/100g), testosterone level (3.37±0.79 vs. 0.61±0.29 ng/ml), and sperm concentration (115.33±7.70 vs. 79.13±3.65×106 cells/ml) of CS were significantly lower (p<0.05) than controls. Some atrophic seminiferous tubules and low sperm mass were apparent in CS rats. The expression of CYP11A1 except StAR protein was markedly decreased in CS rats. In contrast, a 55 kDa phosphorylated protein was higher in CS testes.
Conclusion: CS decreased the expression of CYP11A, resulting in decreased testosterone, and increased acrosome-reacted sperm, assumed to be the result of an increase of 55 kDa phosphorylated protein.
Objective: To investigate the acrosome status and alterations of testicular proteins involved in spermatogenesis and testosterone synthesis in chronic stress in rats.
Materials and Methods: In this experimental study, male rats were divided into 2 groups (control and chronic stress (CS), n=7). CS rats were immobilized (4 hr/day) for 42 consecutive days. The blood glucose level (BGL), corticosterone, testosterone, acrosome status, and histopathology were examined. The expressions of testicular steroidogenic acute regulatory (StAR), cytochrome P450 side chain cleavage (CYP11A1), and phosphorylated proteins were analyzed.
Results: Results showed that BGL (71.25±2.22 vs. 95.60±3.36 mg/dl), corticosterone level (24.33±4.23 vs. 36.9±2.01 ng/ml), acrosome reacted sperm (3.25±1.55 vs. 17.71±5.03%), and sperm head abnormality (3.29±0.71 vs. 6.21±1.18%) were significantly higher in CS group in comparison with control. In contrast, seminal vesicle (0.41±0.05 vs. 0.24±0.07 g/100g), testosterone level (3.37±0.79 vs. 0.61±0.29 ng/ml), and sperm concentration (115.33±7.70 vs. 79.13±3.65×106 cells/ml) of CS were significantly lower (p<0.05) than controls. Some atrophic seminiferous tubules and low sperm mass were apparent in CS rats. The expression of CYP11A1 except StAR protein was markedly decreased in CS rats. In contrast, a 55 kDa phosphorylated protein was higher in CS testes.
Conclusion: CS decreased the expression of CYP11A, resulting in decreased testosterone, and increased acrosome-reacted sperm, assumed to be the result of an increase of 55 kDa phosphorylated protein.
Type of Study: Original Article |
References
1. Retana-Marquez S, Bonilla-Jaime H, Vazquez-Palacios G, Martinez-Garcia R, Velazquez-Moctezuma J. Changes in masculine sexual behavior, corticosterone and testosterone in response to acute and chronic stress in male rats. Horm Behav 2003; 44: 327- 337. [DOI:10.1016/j.yhbeh.2003.04.001]
2. Lin H, Yuan KM, Zhou HY, Bu T, Su H, Liu S, et al. Time-course changes of steroidogenic gene expression and steroidogenesis of rat Leydig cells after acute immobilization stress. Int J Mol Sci 2014; 15: 21028-21044. [DOI:10.3390/ijms151121028]
3. Arun S, Burawat J, Sukhorum W, Sumpannang A, Uabundit N, Iamsaard S. Changes of testicular phosphorylated proteins in response to restraint stress in male rats. J Zhejiang Univ Sci B 2016; 17: 87-95. [DOI:10.1631/jzus.B1500174]
4. Kennedy SH, Dickens SE, Eisfeld BS, Bagby RM. Sexual dysfunction before antidepressant therapy in major depression. J Affect Disord 1999; 56: 201-208. [DOI:10.1016/S0165-0327(99)00050-6]
5. Baldwin DS. Depression and sexual function. Br Med Bull 2001; 57: 81-99. [DOI:10.1093/bmb/57.1.81]
6. Rai J, Pandey SN, Srivastava RK. Testosterone hormone level in albino rats following restraint stress of long duration. J Anat Soc India 2004; 53: 17-19.
7. Fenster L, Katz DF, Wyrobek AJ, Pieper C, Rempel DM, Oman D, et al. Effects of psychological stress on human semen quality. Asian J Androl 1997; 18: 194-202.
8. Clarke RN, Klock SC, Geoghegan A, Travassos DE. Relationship between psychological stress and semen quality among in-vitro fertilization patients. Hum Reprod 1999; 14: 753-758. [DOI:10.1093/humrep/14.3.753]
9. Rai J, Pandey SN, Srivastava RK. Effect of restraint stress on spermatogenesis of albino rats. J Anat Soc India 2003; 52: 55-57.
10. Hirano T, Kobayashi Y, Omotehara T, Tatsumi A, Hashimoto R, Umemura Y, et al. Unpredictable chronic stress-induced reproductive suppression associated with the decrease of kisspeptin immunore activity in male mice. J Vet Med Sci 2014; 76: 1201-1208. [DOI:10.1292/jvms.14-0177]
11. Martin LJ, Tremblay JJ. Glucocorticoids antagonize cAMP-induced Star transcription in Leydig cells through the orphan nuclear receptorNR4A1. J Mol Endocrinol 2008; 41: 165-175. [DOI:10.1677/JME-07-0145]
12. Stojkov NJ, Janjic MM, Bjelic MM, Mihajlovic AI, Kostic TS, Andric SA. Repeated Immobilization stress disturbed steroidogenic machinery and stimulated the expression of cAMP signaling elements and adrenergic receptors in Leydig cells. Am J Physiol Endocrinol Metab 2012; 302: E1239-1251. [DOI:10.1152/ajpendo.00554.2011]
13. Yanagimachi R. Mammalian fertilization. The physiology of reproduction. In: Knobil E. (Ed). Raven Press, New York; 1994.
14. Visconti PE, Kopf GS. Regulation of protein phosphorylation during sperm capacitation. Biol Reprod 1998; 59: 1-6. [DOI:10.1095/biolreprod59.1.1]
15. Tomes CN, Roggero CM, De Blas G, Saling PM, Mayorga LS. Requirement of protein tyrosine kinase and phosphatase activities for human sperm exocytosis. Dev Biol 2004; 265: 399-415. [DOI:10.1016/j.ydbio.2003.09.032]
16. Florman HM, Ducibella T. Fertilization in Mammals. Physiology of Reproduction. In: Neill JD, editor. Elsevier; San Diego CA; 2006: 55-112.
17. Kulanand J, Shivaji S. Capacitation-associated changes in protein tyrosine phosphorylation, hyperactivation and acrosome reaction in hamster spermatozoa. Andrologia 2001; 33: 95-104. [DOI:10.1046/j.1439-0272.2001.00410.x]
18. Iamsaard S, Prabsattroo T, Sukhorum W, Muchimapura S, Srisaard P, Uabundit N, et al. (dill) extract enhances the mounting frequency and level of testicular tyrosine proteinphosphorylation in rats. J Zhejiang UnivSci B 2013; 14: 247-252. [DOI:10.1631/jzus.B1200287]
19. Iamsaard S, Sukhorum W, Samrid R, Yimdee J, Kanla P, Chaisiwamongkol K, et al. The sensitivity of male rat reproductive organs to monosodium glutamate. Acta Med Acad 2014; 43: 3-9. [DOI:10.5644/ama2006-124.94]
20. Kopf G, Gerton G. The mammalian sperm acrosome and the acrosome reaction. Elements of Mammalian Fertilization. In: Wassarman P. (Ed). CRC Press, Boca Raton, USA; 1990: 154-203.
21. Arad-Dann H, Beller U, Haimovitch R, Gavrieli Y, Ben-Sasson SA. Immunohistochemistry of phosphotyrosine residues: identification of distinct intracellular patterns in epithelial and steroidogenic tissues. J Histochem Cytochem 1993; 41: 513-519. [DOI:10.1177/41.4.7680679]
22. Iamsaard S, Burawat J, Kanla P, Arun S, Sukhorum W, Sripanidkulchai B, et al. Antioxidant activity and protective effect of Clitoria ternatea flower extract on testicular damage induced by ketoconazole in rats. J Zhejiang Univ Sci B 2014; 15: 548-555. [DOI:10.1631/jzus.B1300299]
23. Zardooz H, Zahedi Asl S, Gharib Naseri MK, Hedayati M. Effect of chronic restraint stress on carbohydrate metabolism in rat. Physiol Behav 2006; 89: 373-378. [DOI:10.1016/j.physbeh.2006.06.023]
24. Wyrobek AJ, Bruce WR. Chemical induction of sperm abnormalities in mice. Proc Nat Acad Sci 1975; 72: 4425-4429. [DOI:10.1073/pnas.72.11.4425]
25. Sakr SA, Zowail ME, Marzouk AM. Effect of saffron (Crocus sativus L.) on sodium valproate induced cytogenetic and testicular alterations in albinorats. Anat Cell Biol 2014; 47: 171-179. [DOI:10.5115/acb.2014.47.3.171]
26. Bendahmane M, Zeng HT, Tulsiani DR. Assessment of acrosomal status in rat spermatozoa: studies on carbohydrate and non-carbohydrate agonists. Arch Biochem Biophys 2002; 404: 38-47. [DOI:10.1016/S0003-9861(02)00278-3]
27. Izgüt-Uysal VN, Gemici B, Birsen I, Acar N, Ustunel I. The protective effect of apelin against water-immersion and restraint stress-induced gastric damage. J Physiol Sci 2014; 64: 279-289. [DOI:10.1007/s12576-014-0317-8]
28. Orr TE, Mann DR. Role of glucocorticoids in the stress-induced suppression of testicular steroidogenesis in adult male rats. Horm Behav 1992; 26: 350-363. [DOI:10.1016/0018-506X(92)90005-G]
29. Kosti CT, Andri CS, Mari CD. The effect of opiod antagonist in local regulation of testicular response to acute stress in adult rats. Steroids 1997; 62: 703-708. [DOI:10.1016/S0039-128X(97)00071-8]
30. Herman JP, Figueiredo H, Mueller NK, Ulrich-Lai Y, Ostrander MM, Choi DC, et al. Central mechanisms of stress integration: hierarchical circuitry controlling hypothalamo- pituitary- adrenocortical responsiveness. Front Neuro Endocrinol 2003; 24: 151-180. [DOI:10.1016/j.yfrne.2003.07.001]
31. Orr TE, Taylor MF, Bhattacharyya AK, Collins DC, Mann DR. Acute immobilization stress disrupts testicular steroidogenesis in adult male rats by inhibiting the activities of 17 alpha-hydroxylase and 17, 20-lyase without affecting the binding of LH/hCG receptors. J Androl 1994; 15: 302-308.
32. Priya PH, Reddy PS. Effect of restraint stress on lead-induced male reproductive toxicity in rats. J Exp Zool A Ecol Genet Physiol 2012; 317: 455-465. [DOI:10.1002/jez.1738]
33. Yao R, Ito C, Natsume Y, Sugitani Y, Yamanaka H, Kuretake S, et al. Lack of acrosome formation in mice lacking a Golgi protein, GOPC. Cell Biol 2002; 99: 11211-11216. [DOI:10.1073/pnas.162027899]
34. Wang H, Wan H, Li X, Liu W, Chen Q, Wang Y, et al. Atg7 is required for acrosome biogenesis during spermatogenesis in mice. Cell Res 2014; 24: 852-869. [DOI:10.1038/cr.2014.70]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
