جمعه 31 فروردین 1403
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دوره 5، شماره 4 - ( 4-1386 )
جلد 5 شماره 4 صفحات 133-127 |
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Soleimani Mehranjani M, Hemadi M. The effects of sodium arsenite on the testis structure and sex hormones in vasectomised rats. IJRM 2007; 5 (4) :127-133
URL: http://ijrm.ir/article-1-78-fa.html
URL: http://ijrm.ir/article-1-78-fa.html
The effects of sodium arsenite on the testis structure and sex hormones in vasectomised rats. International Journal of Reproductive BioMedicine. 1386; 5 (4) :127-133
چکیده: (2251 مشاهده)
Background: Sodium arsenite and/ or vasectomy may cause variation in sex hormones which affect pathophysiology of reproductive organs.
Objective: The aim was to investigate the morphological changes in structure of testis and hormonal imbalance in bilateral Vasectomised rats treated with sodium arsenite.
Materials and Methods: Four groups of rats: bilateral vasectomy + sodium arsenite, bilateral vasectomy, sham operated + sodium arsenite and sham operated only were considered, and 8 mg/kg/ day of sodium arsenite was given for 8 weeks to the rats. The total volume of testis, volume of interstitial tissue, volume of seminiferous tubules, diameter of seminiferous tubules and germinal epithelium thickness were evaluated using stereological methods. Hormones were also measured and the results were analyzed using one way ANOVA.
Results: A significant reduction of total volume of testis (p<0.01), mean volume of seminiferous tubules (p<0.002) as well as germinal epithelium thickness (p<0.05) in both vasectomy + sodium arsenite and vasectomy rats was seen compared to sham operated only. In addition a significant reduction of testosterone was observed in vasectomy + sodium arsenite group when compared to the other groups (p<0.001). LH level decreased significantly in vasectomy + sodium arsenite when compared to sham operated ones (p<0.05).
Conclusion: Vasectomy and treatment with sodium arsenite affect the structure of testis with respect to its volume, volume of seminiferous tubules and thickness of germinal epithelium, which may be due to variation of LH and testosterone level in the rats.
Objective: The aim was to investigate the morphological changes in structure of testis and hormonal imbalance in bilateral Vasectomised rats treated with sodium arsenite.
Materials and Methods: Four groups of rats: bilateral vasectomy + sodium arsenite, bilateral vasectomy, sham operated + sodium arsenite and sham operated only were considered, and 8 mg/kg/ day of sodium arsenite was given for 8 weeks to the rats. The total volume of testis, volume of interstitial tissue, volume of seminiferous tubules, diameter of seminiferous tubules and germinal epithelium thickness were evaluated using stereological methods. Hormones were also measured and the results were analyzed using one way ANOVA.
Results: A significant reduction of total volume of testis (p<0.01), mean volume of seminiferous tubules (p<0.002) as well as germinal epithelium thickness (p<0.05) in both vasectomy + sodium arsenite and vasectomy rats was seen compared to sham operated only. In addition a significant reduction of testosterone was observed in vasectomy + sodium arsenite group when compared to the other groups (p<0.001). LH level decreased significantly in vasectomy + sodium arsenite when compared to sham operated ones (p<0.05).
Conclusion: Vasectomy and treatment with sodium arsenite affect the structure of testis with respect to its volume, volume of seminiferous tubules and thickness of germinal epithelium, which may be due to variation of LH and testosterone level in the rats.
نوع مطالعه: Original Article |
فهرست منابع
1. Liu SX, Athar M, Lippai I, Waldren C, Hei TK. Induction of oxyradicals by arsenic: implication for mechanism of genotoxicity. Proc Natl Acad Sci USA 2001; 8: 1643-1648. [DOI:10.1073/pnas.98.4.1643]
2. Bates MN, Smith AH, Hopenhayn-Rich C. Arsenic ingestion and internal cancers: a review. Am J Epidemiol 1992; 135:462- 476. [DOI:10.1093/oxfordjournals.aje.a116313]
3. Jana K, Jana S, Samanta PK. Effect of chronic exposure to sodium arsenite on hypothalamo- pituitary- testicular activities in adult rats: possible an estrogenic mode of action. Repord Biol Endocrinol 2006; 4: 9. [DOI:10.1186/1477-7827-4-9]
4. Pant N, Murthy RC, Srivastava SP. Male reproductive toxicity of sodium arsenite in mice. Human and Experimental Toxicology 2004; 23: 399-403. [DOI:10.1191/0960327104ht467oa]
5. Geierhaas B, Bornstein SR, Jarry H, Scherbaum WA, Herrmann M, Pfeiffer EF. Morphological and hormonal changes following vasectomy in rats, suggesting a functional role for Leydig-cell associated. macrophages. Horm Metab Res 1991; 23: 373-8. [DOI:10.1055/s-2007-1003704]
6. Pires Das Neves RN, Carvalho F, Carvalho M, Fernandes E, Soares E, Bastos MDL, Pereira MDL. Protective activity of hesperidin and lipoic acid against sodium arsenite acute toxicity in mice . Toxicologic Pathology 2004; 32: 527-535. [DOI:10.1080/01926230490502566]
7. Ikeda T, Sofikitis N. Bilateral testicular consequences in the unilateral vasectomy of immature rats. Yonago Acta Medica 2000; 43: 1-9.
8. Sarkar M, Chaudhuri GR, Chattopadhyay A, Biswas NM. Effect of sodium arsenite on spermatogenesis, plasma gonadotrophins and testosterone in rats. Asian J Androl 2003; 1: 27-31.
9. Chattopadhyay S, Ghosh S, Chaki S, Debnath J, Ghosh D. Effect of sodium arsenite on plasma levels of gonadotrophins and ovarian steroidogenesis in mature albino rats: duration dependent response. J Toxicol Sci 1999; 24:425-431. [DOI:10.2131/jts.24.5_425]
10. Ikeda T, Sofikitis N. Bilateral testicular consequences in the unilateral vasectomy of immature rats. Yonago Acta medica 2000; 43: 1-9.
11. Gundersen H J, Bagger P, Bendtsen TF, Evans SM., Korbo L, Marcussen N, et al. The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. APMIS (1988a) 96, 857-881. [DOI:10.1111/j.1699-0463.1988.tb00954.x]
12. Gundersen HJ, Bendtsen TF, Korbo L, Marcussen N, Moller A, Nielsen K et al. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS 1988b; 96: 379-94. [DOI:10.1111/j.1699-0463.1988.tb05320.x]
13. Mouton PR. Principles and practices of unbiased stereology: an introduction for bioscientists. The Johns Hopkins University Press. Baltimore/London 2002.
14. Howard CV, Reed MG. Unbiased stereology Three-dimensional measurement in microscopy. Oxford: Bios Scientific Publishers 1998; pp: 143.
15. Gunderson HJG, Jensen EB. Stereological estimation of the volume-weighted mean volume of arbitrary particles observed on random sections. J. Microsc 1985; 138: 127-142. [DOI:10.1111/j.1365-2818.1985.tb02607.x]
16. Jensen EB, Gundersen HJG. Fundamental stereological formula based on isotropically oriented probes through fixed points with applications to particle analysis. J Microsc 1989; 153: 249-267. [DOI:10.1111/j.1365-2818.1989.tb01476.x]
17. Pant N, Kumar R, Murthy RC, Srivastava SP. Male reproductive effect of arsenic in mice. Biometals 2001; 14: 113-117. [DOI:10.1023/A:1016686113763]
18. Sarkar M, Biswas NM, Ghosh D. Effect of sodium arsenite on testicular and 3beta and 17beta hydroxysteroid dehydrogenase activation in albino rats: dose and duration dependent response. Med Sci Res 1991; 19: 789-793.
19. Chowdhury AK. Dependence of testicular germ cells on hormones: a quantitative study in hypophysectomized testosterone treated rats. J Androl 1979; 82: 331-340. [DOI:10.1677/joe.0.0820331]
20. Neaves WB. The rat testis after vasectomy. J Reporod Fert 1974; 40: 39-44. [DOI:10.1530/jrf.0.0400039]
21. Chang SI, Jin B, Youn P, Park C, Park JD, Ryu DY. Arsenic-induced toxicity and the protective role of ascorbic acid in mouse testis. Toxicology and Applied Pharmacology 2007; 218: 196-203. [DOI:10.1016/j.taap.2006.11.009]
22. Sharpe RM, Donachie k, Cooper I. Re-evaluation of the intratesticular level of testosterone required for quantitative maintenance of spermatogenesis in the rat. J Endocrinol 1988; 117: 19-26. [DOI:10.1677/joe.0.1170019]
23. Sharpe RM, Maddocks S, Millar M, Saunders PTK, Kerr JB, Mckinnell C. Testosterone and spermatogenesis: identification of stage dependent, androgen- regulated proteins secreted by adult rat seminiferous tubules. J Androl 1992; 13: 172-184.
24. Kim JM, Ghosh SR, Weil ACP, Zirkin BR. Caspase-3 and caspase- activated dioxyribonuclease are associated with testicular germ cell apoptosis resulting from reduced intratesticular testosterone. Endocrinology 2001; 142: 3809-3816. [DOI:10.1210/endo.142.9.8375]
25. Blanco- Rodriguez J, Martinez- Garcia C. Apoptosis precedes detachment of germ cells from the seminiferous epithelium after hormonal suppression by short-term oestradiol treatment of rats. Int J Androl 1998; 21: 109-115. [DOI:10.1046/j.1365-2605.1998.00109.x]
26. Shiraishi K, Takihara H, Naito K. Quantitative Analysis of Testicular Interstitial Fibrosis after Vasectomy in Humans. Aktuel Urol 2003; 34: 262-264. [DOI:10.1055/s-2003-41612]
27. Pérez-Marín CC, López R, Domínguez JM, Zafra R. Clinical and pathological findings in testis, epididymis, deferens duct and prostate following vasectomy in a dog. Reprod Dom Anim 2006; 41: 169-174. [DOI:10.1111/j.1439-0531.2006.00662.x]
28. Jarow JP, Budin RE, Dym M, Zirkin BR, Noren S, Marshall FF. Quantitative pathologic changes in the human testis after vasectomy. A controlled study. N Engl J Med 1985; 313: 1252-1256. [DOI:10.1056/NEJM198511143132003]
29. Shiraishi K, Takihara H, Naito K. Influence of interstitial fibrosis on spermatogenesis after vasectomy and vasovasostomy. Contraception 2002; 65: 245-249. [DOI:10.1016/S0010-7824(01)00311-0]
30. Yamanaka K, Fujisawa M, Tanaka H, Okada H, Arakawa S, Kamidono S. Significance of human testicular mast cells and their subtypes in male infertility. Hum Reprod 2000; 15: 1543-1547. [DOI:10.1093/humrep/15.7.1543]
31. Raleigh D, O'Donnell L, Southwick GJ, de Kretser DM, McLachlan RI. Stereological analysis of the human testis after vasectomy indicates impairment of spermatogenic efficiency with increasing obstructive interval. Fertil Steril 2004, 81(6):1595-603. [DOI:10.1016/j.fertnstert.2003.10.046]
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