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Volume 5, Issue 4 (7-2007)
IJRM 2007, 5(4): 89-93 |
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Mohammadzadeh A, Heidari M, Soltan Ghoraiee H, Ghaffari Novin M, Jeddi-Tehrani M, Akhondi M M, et al . The effect of pentoxifylline on the growth of endometrial implants and leukocytes in rats.. IJRM 2007; 5 (4) :89-93
URL: http://ijrm.ir/article-1-82-en.html
URL: http://ijrm.ir/article-1-82-en.html
Afsaneh Mohammadzadeh * 
1, Mahnaz Heidari2 , Haleh Soltan Ghoraiee3 , Marefat Ghaffari Novin4 , Mahmood Jeddi-Tehrani5 , Mohammad Mahdi Akhondi5 , Hojjat Zeraati5 , Farzaneh Mohammadzadeh6 , Pegah Ebadi4
1, Mahnaz Heidari2 , Haleh Soltan Ghoraiee3 , Marefat Ghaffari Novin4 , Mahmood Jeddi-Tehrani5 , Mohammad Mahdi Akhondi5 , Hojjat Zeraati5 , Farzaneh Mohammadzadeh6 , Pegah Ebadi4
1- Department of Reproductive Endocrinology and Embryology, Reproductive Biotechnology Research Center, Avesina Research Institute, ACECR, Tehran, Iran , af23mohammadzadeh@yahoo.com
2- Departmet of Nanotechnology , Nanobiotechnology Research Center , Avesina Research Institute, ACECR, Tehran, Iran
3- Department of Reproductive and Biotechnology Genetic, Reproductive Biotechnology Research Center, Avesina Research Institute ,ACECR, Tehran, Iran
4- Department of Reproductive Endocrinology and Embryology, Reproductive Biotechnology Research Center, Avesina Research Institute, ACECR, Tehran, Iran
5- Department of Monoclonal Antibody, Monoclonal Antibody Research Center , Avesina Research Institute ,ACERCR, Tehran, Iran
6- Anatomy Department, Iran University of Medical Science, Tehran, Iran
2- Departmet of Nanotechnology , Nanobiotechnology Research Center , Avesina Research Institute, ACECR, Tehran, Iran
3- Department of Reproductive and Biotechnology Genetic, Reproductive Biotechnology Research Center, Avesina Research Institute ,ACECR, Tehran, Iran
4- Department of Reproductive Endocrinology and Embryology, Reproductive Biotechnology Research Center, Avesina Research Institute, ACECR, Tehran, Iran
5- Department of Monoclonal Antibody, Monoclonal Antibody Research Center , Avesina Research Institute ,ACERCR, Tehran, Iran
6- Anatomy Department, Iran University of Medical Science, Tehran, Iran
Abstract: (2952 Views)
Background: Immune system disturbances have an important role in endometriosis which may lead to infertility. It seems that inflammatory cytokines specially tumor necrosis factor alpha (TNF-a) which were produced by activated macrophages have an important role in pathology of endometriosis. Based on this theory, anti TNF-a drugs like pentoxifylline (PX) are suggested as new drugs for Endometriosis.
Objective: This experimental study has been done on female rats to determine the effect of PX on the endometrial implants and leukocytes in serum.
Material and Methods: In proestrous phase, one horn of rat’s bicorn uterus was removed surgically and the endometrium was implanted to different places as follows: subcutaneous, peritoneum and near ovaries. After two months observation, female rats divided into two groups randomly. In treated group (n=10) PX (5mg/kg twice a day) and in control group (n=10), normal saline (same dose) were injected subcutaneously. Then, via second laparotomy and in the same phase of the cycles, the size of implants and the amount of leukocytes in serum were measured.
Results: In treated group compared with control, the size of implants was decreased significantly in right subcutaneous (8.05mm vs 13.50mm) p<0.01, left subcutaneous (7.64 mm vs 14mm) p<0.01, right ovary (6.64 mm vs 15.22mm) p<0.001 and left ovary (7.18 mm vs 14.56 mm) p<0.005. In treated group, the total leukocyte count (5259.54 ± 178.78 vs 15833.33 ± 259.27) p<0.02 was decreased. The number of esterous cycle was similar in both groups.
Conclusion: PX can reduce the size of endometrial implants as well as leukocyte count.
Objective: This experimental study has been done on female rats to determine the effect of PX on the endometrial implants and leukocytes in serum.
Material and Methods: In proestrous phase, one horn of rat’s bicorn uterus was removed surgically and the endometrium was implanted to different places as follows: subcutaneous, peritoneum and near ovaries. After two months observation, female rats divided into two groups randomly. In treated group (n=10) PX (5mg/kg twice a day) and in control group (n=10), normal saline (same dose) were injected subcutaneously. Then, via second laparotomy and in the same phase of the cycles, the size of implants and the amount of leukocytes in serum were measured.
Results: In treated group compared with control, the size of implants was decreased significantly in right subcutaneous (8.05mm vs 13.50mm) p<0.01, left subcutaneous (7.64 mm vs 14mm) p<0.01, right ovary (6.64 mm vs 15.22mm) p<0.001 and left ovary (7.18 mm vs 14.56 mm) p<0.005. In treated group, the total leukocyte count (5259.54 ± 178.78 vs 15833.33 ± 259.27) p<0.02 was decreased. The number of esterous cycle was similar in both groups.
Conclusion: PX can reduce the size of endometrial implants as well as leukocyte count.
Type of Study: Original Article |
References
1. Akanda VA, Hunt LP, Cahill DJ, Jenkins JM. Differences in time to natural conception between women with unexplained infertility and infertile women with minor endometriosis. Hum Reprod 2004 ;19:96-103. [DOI:10.1093/humrep/deh045] [PMID]
2. Denny E, Khan KS. Systematic reviews of qualitative evidence. What are the experiences of women with endometriosis. J Obstet Gynaecol 2006;26:501-506. [DOI:10.1080/01443610600797301] [PMID]
3. Tomassetti C, Meuleman C, Pexster SA, Mihalyi A, Kyama C, Sima P, D'Hooghe TM. Endometriosis, recurrent miscarriage and implantation failure : is there an immunological link? Reprod Biomed Online 2006;13:58-64. [DOI:10.1016/S1472-6483(10)62016-0]
4. Berkkangolu M, Arici A. Immunology and endometriosis . Am J Reprod.Immunol 2003;50:48-59. [DOI:10.1034/j.1600-0897.2003.00042.x] [PMID]
5. Nothnick WB. Treating endometriosis as an autoimmune disease. Fertil Steril 2001;76 :223-231. [DOI:10.1016/S0015-0282(01)01878-7]
6. Kyama.CM, Debrock S, Mwenda JM, D'Hooghe TM. Potential involvement of the immune system in development of endometriosis .Reprod Biol Endocrinol 2003;1:123-139. [DOI:10.1186/1477-7827-1-123] [PMID] [PMCID]
7. Maeda N, Lzumiya C, Yamamoto Y, Yomamato Y, Oguri H, Kusume T, Fukaya T. Increased killer inhibitory receptor KIR2DL1 expression among natural killer cells in women with pelvic endometriosis .Fertil Steril 2002 ;77:297-302. [DOI:10.1016/S0015-0282(01)02964-8]
8. Gomez-Tores MJ, Acien P, Campos A, Velasco I. Embriotoxicity of peritoneal fluid in women with endometriosis: Its relation with cytokins and lymphocyte population.Hum Reprod 2002 ;17: 777-781. [DOI:10.1093/humrep/17.3.777] [PMID]
9. Richter O, Mallman P, Vanderven H, Krebs D. TNF-α Secretion by Peritoneal macrophages in endometriosis. Zentralbl Gynakol 1998;120:332-336.
10. Jerzak M,Baranowski W,Rechberger T, Gorski A. Enhanced Tcell interactions with extracellular matrix proteins in infertile women with endometriosis. Immunol Lett 2002; 81:65-70. [DOI:10.1016/S0165-2478(01)00337-6]
11. Bruner KL, Matrstan IM, Rodgers WI, Gorstein F, Osteen K. Suppression of matrix metalloproteinase inhibits establishment of ectopic lesions by human tissue in nude mice. J Clin Invest 1997; 99: 2851-2857. [DOI:10.1172/JCI119478] [PMID] [PMCID]
12. Sullivan GW, Caper HT, Novick WJ Jr, Mandell GL. Induction of the inflammatory action of IL1, TNF-α, on neutrofil function by Pentoxifylline. Infect Immun. 1988 ;56:1722-1729.
13. Ward A, Clissold SP. Pentoxifylline .A review of it's pharmacodynamic and pharmacokinetic properties and it's therapeutic efficacy. Drugs 1987;34:50-97. [DOI:10.2165/00003495-198734010-00003] [PMID]
14. Maksymowych WP, Avina-Zubeta A, Luong MH, Kussel AS. An open study of pentoxifylline in the treatment of sever refractory rheumatoid arthritis. J Rheumatol 1995; 22:625-629.
15. Reimund JM, Bumont S, Muller CD, Kenny JS, kedinger M, Bauman R, et al . In vitro effects of pentoxifylline on inflammatory cytokine release in patients with inflammatory bowel disease. Gut 1997 ;40: 475-480. [DOI:10.1136/gut.40.4.475] [PMID] [PMCID]
16. Thornhill MH, Baccaglini L, Theaker E, Pemberton MN. A randomized Double-blind Placcebo-controlled of Pentoxifylline for the treatment of Recurrent Aphthous Stomatitis. Arch Dermatol 2007;143:463-470. [DOI:10.1001/archderm.143.4.463] [PMID]
17. Vernon MW, Wilson EA. Studies on the surgical induction of endometriosis in the rat. Fertil steril 1985 ;44 :684-699.
18. Sharpe- Timms KL. Using rats as a research model for the study of endometriosis. Ann NY Acad Sci 2002 ;955:318-327. [DOI:10.1111/j.1749-6632.2002.tb02792.x] [PMID]
19. Steinleitner A, Lambert H, Suarez M, Serpa N, Roy S. Immunomodulation in the treatment of endometriosis - associated subfertility : use of Pentoxifylline to reverse the inhibition of fertilization by surgically induced endometriosis in a rodent model. Fertil Steril 1991;56:975-979. [DOI:10.1016/S0015-0282(16)54674-3]
20. Nothnick WB, Curry TE, Vernon MW. Immunomodulation of rat endometriotic implant growth and protein production. AJRI 1994;31:151-162. [DOI:10.1111/j.1600-0897.1994.tb00860.x] [PMID]
21. Balasch J, Creus M, Fabregues F, Carmona F, Martinez-Roman S, Manau D, Vanrell JA. Pentoxifylline versus placebo in the treatment of infertility associated with minimal or mild endometriosis: a pilot randomized clinical trial. Hum Reprod 1997;12:2046-2050. [DOI:10.1093/humrep/12.9.2046] [PMID]
22. Alborzi S, Ghotbi S, Parsanezhad ME, Dehbashi S, Alborzi S, Alborzi M. Pentoxifylline therapy after laparoscopic surgery for different stages of endometriosis: a prospective, double blind,randomized placebo-controled study. J Minim Invasive Gynecol 2007;14:54-58. [DOI:10.1016/j.jmig.2006.06.024] [PMID]
23. Smith MG, Hau J, Hoosier GL. Handbook of laboratory animal science, 2nd ed , U.S.A, CRC press . 2003.p. 51-62.
24. Waggie K. Manual of microbiologic monitoring of laboratory animals, 2nd ed , U.S.A, DIAN publishing. 1996:59-165.
25. Mohammadzadeh A, Heidari M, Soltan Ghoraii H, Zarnani AH, Ghaffari Novin M, Akhondi MM Mossavie Jarahi A, Mohammadzadeh F. Induction of endometriosis by implantation of endometrial fragments in female rats. IJRM 2006; 4:63-67.
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