Volume 15, Issue 5 (6-2017)                   IJRM 2017, 15(5): 297-304 | Back to browse issues page

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Fesahat F, Montazeri F, Sheikhha M H, saeedi H, Dehghani Firouzabadi R, Kalantar S M. Frequency of chromosomal aneuploidy in high quality embryos from young couples using preimplantation genetic screening. IJRM. 2017; 15 (5) :297-304
URL: http://ijrm.ssu.ac.ir/article-1-821-en.html
1- Genetics Department, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3- Embryology Department, Omid Fertility Clinic, Tehran, Iran
4- Genetics Department, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , smkalantar@yahoo.com
Abstract:   (2443 Views)
Background: Selection of the best embryo for transfer is very important in assistedreproductive technology (ART). Using morphological assessment for this selectiondemonstrated that the correlation between embryo morphology and implantationpotential is relatively weak. On the other hand, aneuploidy is a key genetic factorthat can influence human reproductive success in ART.
Objective: The aim of this lab trial study was to evaluate the incidence ofaneuploidies in five chromosomes in the morphologically high-quality embryosfrom young patients undergoing ART for sex selection.
Materials and Methods: A total of 97 high quality embryos from 23 women at theage of 37or younger years that had previously undergone preimplantation geneticscreening for sex selection were included in this study. After washing, the slides ofblastomeres from embryos of patients were reanalyzed by fluorescence in-situhybridization for chromosomes 13, 18 and 21.
Results: There was a significant rate of aneuploidy determination in the embryosusing preimplantation genetic screening for both sex and three evaluated autosomalchromosomes compared to preimplantation genetic screening for only sexchromosomes (62.9% vs. 24.7%, p=0.000). The most frequent detectedchromosomal aneuploidy was trisomy or monosomy of chromosome 13.
Conclusion: There is considerable numbers of chromosomal abnormalities inembryos generated in vitro which cause in vitro fertilization failure and it seems thatmorphological characterization of embryos is not a suitable method for choosing theembryos without these abnormalities.
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Type of Study: Original Article |

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