Volume 2, Issue 1 (7-2004)                   IJRM 2004, 2(1): 34-39 | Back to browse issues page

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Zarghami N, Khosrowbeygi A. Evaluation of Lipid Peroxidation as an Indirect Measure of Oxidative Stress in Seminal Plasma. IJRM. 2004; 2 (1) :34-39
URL: http://ijrm.ssu.ac.ir/article-1-9-en.html
Abstract:   (2197 Views)
Background: It has been proposed that oxidative stress plays an important role in male infertility. The aims of this study were to compare seminal plasma levels of 15-F2t-isoprostane (8-iso-PGF2?), malondialdehyde (MDA), and total (sum of free and bound) homocysteine (tHcy) in normozoospermic vs. asthenozoospermic men, and to examine the relationships between tHcy and lipid peroxidation products. Materials and Methods: The study was a case-control study with a simple random sampling. The case group consisted of 15 asthenozoospermic males. This group was compared with 15 normozoospermic men. Seminal plasma levels of 15-F2t-isoprostane and tHcy were measured using commercially available enzyme immunoassay (EIA) kits. MDA levels were determined by the thiobarbituric acid (TBA) assay. The Mann-Whitney U test was used to compare two groups. Coefficients of correlation were calculated using Spearman’s correlation analysis. All hypothesis tests were two-tailed with statistical significance assessed at the p value <0.05 level. Results: MDA levels were lower in asthenozoospermic subjects than in control subjects (0.72±0.06 µM vs. 0.40±0.06 µM; p<0.05). No differences were seen in 15-F2t-isoprostane levels in asthenozoospermic subjects and controls (65.00±3.20 pg/ml vs. 58.17±4.12 pg/ml; p>0.05). Interestingly, tHcy levels were slightly higher in asthenozoospermic subjects than in controls (6.18±1.17 µM vs. 4.8±0.52µM). Sperm motility was inversely correlated with seminal plasma 15-F2t-isoprostane and MDA levels, respectively (p<0.05). Conclusion: Seminal plasma levels of 15-F2t-isoprostane and tHcy showed no significant differences between normozoospermic and asthenozoospermic men. Sperm motility correlated inversely with seminal plasma levels of 15-F2t-isoprostane and MDA. No relationship was found between tHcy and lipid peroxidation. However, higher sample size is required to confirm these findings.
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