Volume 15, Issue 12 (12-2017)                   IJRM 2017, 15(12): 749-756 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Carkci S, Onalan Etem E, Ozaydin S, Karakeci A, Tektemur A, Ozan T et al . Ion channel gene expressions in infertile men: A case-control study. IJRM. 2017; 15 (12) :749-756
URL: http://ijrm.ssu.ac.ir/article-1-923-en.html
1- Department of Urology, Faculty of Medicine, Firat University, Elazig, Turkey
2- Department of Medical Biology, Faculty of Medicine, Firat University, Elazig, Turkey
3- Department of Medical Biology, Faculty of Medicine, Firat University, Elazig, Turkey.
4- Department of Urology, Faculty of Medicine, Firat University, Elazig, Turkey.
5- Department of Medical Biology, Faculty of Medicine, Firat University, Elazig, Turkey , akarakeci@firat.edu.tr
Abstract:   (1947 Views)
Background: Infertility is described as not receiving pregnancy despite unprotected and regular sexual intercourse in a 1 yr period. It is detected by 15% of the couples. Male and female factor in the etiology may be detected in similar rates.
Objective: The present study aims to investigate ion channel gene expression in semen samples of infertile male compared with fertile men.
Materials and Methods: A total of 150 men who applied to the urology clinic due to infertility were divided into five equal groups: asthenozoospermia, oligozoospermia, oligoasthenoteratozoospermia, teratozoospermia, and normozoospermia (control). All paticipants were evaluated with Cation Channel Spermia (CatSper) 1, 2, 3, 4, Proton Voltage Gated Ion Channel1 (Hv1), Potassium Channel Subfamily U1 (KCNU1), and transmembrane protein (TMEM16A) gene expression in semen samples.
Results: “CatSper1, 4, HV1, KCNU1, and TMEM16A gene expression were detected higher in the oligozoospermia group compared to the controls. CatSper1, 2, 3, 4, KCNU1, and TMEM16A gene expression in the asthenozoospermia group and CatSper1, 2, 3, 4, KCNU1, and TMEM16A gene expression in the teratozoospermia group were detected lower compared to the controls. CatSper1, 4, HV1, and TMEM16A gen expression were higher in the oligoasthenoteratozoospermia men than the controls while CatSper3 gen expression was detected as lower.”
Conclusion: It was detected that these ion channels have an effect on sperm progressive motility and morphology. It may be considered that mutations in these ion channels may result in infertility
Full-Text [PDF 452 kb]   (573 Downloads) |   |   Full-Text (HTML)  (358 Views)  
Type of Study: Original Article |

1. Shukla KK, Mahdi AA, Rajender S. Ion channels in sperm physiology and male fertility and infertility. J Androl 2012; 33: 777-788. [DOI:10.2164/jandrol.111.015552]
2. Yuyan L, Junqing W, Wei Y, Weijin Z, Ersheng G. Are serum zinc and copper levels related to semen quality? Fertil Steril 2008; 89: 1008-1011. [DOI:10.1016/j.fertnstert.2007.04.028]
3. Singh AP, Rajender S. CatSper channel, sperm function and male fertility. Reprod Biomed Online 2015; 30: 28-38. [DOI:10.1016/j.rbmo.2014.09.014]
4. Giojalas LC, Rovasio RA. Mouse spermatozoa modify their motility parameters and chemotactic response to factors from the oocyte microenvironment. Int J Androl 1998; 21: 201-206.
5. Lishko PV, Kirichok Y, Ren D, Navarro B, Chung JJ, Clapham DE. The control of male fertility by spermatozoan ion channels. Ann Rev Physiol 2012; 74: 453-475. [DOI:10.1146/annurev-physiol-020911-153258]
6. Santi CM, Orta G, Salkoff L, Visconti PE, Darszon A, Trevi-o CL. K+ and Cl-Channels and Transporters in Sperm Function. Curr Top Dev Biol 2013; 102: 385-421. [DOI:10.1016/B978-0-12-416024-8.00014-3]
7. World Health Organization, Department of Reproductive Health and Research. WHO laboratory manual for the examination and processing of human semen. 5th Ed. World Health Organization, Switzerland; 2010.
8. Georgiadis AP, Kishore A, Zorrilla M, Jaffe TM, Sanfilippo JS, Volk E, et al. High quality RNA in semen and sperm: isolation, analysis and potential application in clinical testing. J Urol 2015 193: 352-359. [DOI:10.1016/j.juro.2014.07.107]
9. Perdrix A, Travers A, Chelli MH, Escalier D, Do Rego JL, Milazzo JP, et al. Assessment of acrosome and nuclear abnormalities in human spermatozoa with large vacuoles. Hum Reprod 2011; 26: 47-58. [DOI:10.1093/humrep/deq297]
10. Shu F, Zhou X, Li F, Lu D, Lei B, Li Q, et al. Analysis of the correlation of CATSPER single nucleotide polymorphisms (SNPs) with idiopathic asthenospermia. J Assist Reprod Genet 2015; 32: 1643-1649. [DOI:10.1007/s10815-015-0548-5]
11. Ren D, Navarro B, Perez G, Jackson AC, Hsu S, Shi Q, et al. A. Sperm ion channel required for sperm motility and male fertility. Nature 2001; 413: 603-609. [DOI:10.1038/35098027]
12. Tamburrino L, Marchiani S, Minetti F, Forti G, Muratori M, Baldi E. The CatSper calcium channel in human sperm: relation with motility and involvement in progesterone-induced acrozome reaction. Hum Reprod 2014; 29: 418-428. [DOI:10.1093/humrep/det454]
13. Marquez B, Ignotz G, Suarez SS. Contributions of extracellular and intracellular Ca+2 to regulation of sperm motility: release of intracellular stores can hyperactivate CatSper1 and CatSper2 null sperm. Dev Biol 2007; 303: 214-221. [DOI:10.1016/j.ydbio.2006.11.007]
14. Avenarius MR, Hildebrand MS, Zhang Y, Meyer NC, Smith LL, Kahrizi K, et al. Human male infertility caused by mutations in the CATSPER1 channel protein. Am J Hum Genet 2009; 84: 505-510. [DOI:10.1016/j.ajhg.2009.03.004]
15. Bhilawadikar R, Zaveri K, Mukadam L, Naik S, Kamble K, Modi D, et al. Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility, fertilization rate, embryo quality and pregnancy rate. J Assist Reprod Genet 2013; 30: 513-523. [DOI:10.1007/s10815-013-9972-6]
16. Hildebrand MS, Avenarius MR, Fellous M, Zhang Y, Meyer NC, Auer J, et al. Genetic male infertility and mutation of CatSper ion channels. Eur J Hum Genet 2010; 18: 1178-1184. [DOI:10.1038/ejhg.2010.108]
17. Jin J, Jin N, Zheng H, Ro S, Tafolla D, Sanders KM, et al. Catsper3 and Catsper4 are essential for sperm hyperactivated motility and male fertility in the mouse. Biol Reprod 2007; 77: 37-44. [DOI:10.1095/biolreprod.107.060186]
18. Lishko PV, Kirichok Y. The role of Hv1 and CatSper channels in sperm activation. J Physiol 2010; 588: 4667-4672. [DOI:10.1113/jphysiol.2010.194142]
19. Zeng XH, Yang C, Kim ST, Lingle CJ, Xia XM. Deletion of the Slo3 gene abolishes alkalizationactivated K+ current in mouse spermatozoa. Proc Natl Acad Sci USA 2011; 108: 5879-5884. [DOI:10.1073/pnas.1100240108]
20. Santi CM, Martinez-Lopez P, de la Vega-Beltran JL, Butler A, Alisio A, Darszon A, et al. The SLO3 sperm-specific potassium channel plays a vital role in male fertility. FEBS Lett 2010; 584: 1041-1046. [DOI:10.1016/j.febslet.2010.02.005]
21. Orta G, Ferreira G, Jose O, Trevino CL, Beltrán C, Darszon A. Human spermatozoa possess a calcium-dependent chloride channel that may participate in the acrozomal reaction. J Physiol 2012; 590: 2659-2675. [DOI:10.1113/jphysiol.2011.224485]
22. Zanetti N, Mayorga LS. Acrosomal swelling and membrane docking are required for hybrid vesicle formation during the human sperm acrosome reaction. Biol Reprod 2009; 81: 396-405 [DOI:10.1095/biolreprod.109.076166]

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb