Volume 6, Issue 2 (7-2008)                   IJRM 2008, 6(2): 1-0 | Back to browse issues page

XML Print

Vitamins C, E and lipid peroxidation levels in sperm and seminal plasma of asthenoteratozoospermic and normozoospermic men
Mohammad Nouri * 1, Aliyeh Ghasemzadeh2 , Laya Farzadi2 , Vahideh Shahnazi2 , Marefat Ghaffari Novin2
1- Biochemistry Department, Tabriz University of Medical Sciences, Tabriz, Iran , Nourimd@yahoo.com
2- Infertility Center, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract:   (3375 Views)
Background: It has been shown that reactive oxygen species (ROS) can lead to deleterious effects on a range of sperm parameters. Vitamins E and C are capable of reducing ROS levels and lipid peroxidation. Objective: The aim of study was to determine the level of lipid peroxidation as indicated by Malondialdehyde (MDA) and vitamins E and C in sperm and seminal plasma of asthenoteratozoospermic and normozoospermic men and their relationships with semen parameters. Materials and Methods: Forty men with normozoospermic and 60 infertile men with asthenoteratozoospermic semen profiles were randomly selected. Semen analysis was done according to the WHO standard. Sex hormonal profiles were measured by ELISA methods. The level of vitamins (C and E) and MDA were measured by HPLC and tiobarbiotic acid, respectively. Results: MDA concentration in the spermatozoa (0.10.06 nmol/ml) and seminal plasma (1.90.35 nmol/ml) of asthenoteratozoospermic were significantly higher than in normozoospermic males (p<0.001).The level of vitamins E and C in seminal plasma of normozoospermic were significantly higher than in asthenoteratozoospermic males (p<0.01). However, the amount of vitamin C in the spermatozoa of normozoospermic was significantly less than in asthenoteratozoospermic males (p<0.01). Sensitivity, specificity, positive and negative prognostic value of MDA of seminal plasma and spermatozoa were more than vitamins C and E. The level of vitamin C of spermatozoa had more diagnostic value when compare to vitamins C and E in seminal plasma. Conclusion: The level of MDA in seminal plasma and spermatozoa and vitamin C in spermatozoa may be a diagnostic tool for etiology of infertility in the asthenoteratozoospermic patients.
Keywords: Asthenoteratozoospermia, MDA, Vitamin E, Vitamin C, Lipid peroxidation, Antioxidants activity, Infertility.
Full-Text [PDF 47 kb]   (694 Downloads) |   |   Full-Text (HTML)  (402 Views)  
Type of Study: Original Article |
References
1. Aitken RJ, Fisher H. Reactive oxygen species generation and human spermatozoa: the balance of benefit and risk. Bioassays 1994; 16: 259-267. [DOI:10.1002/bies.950160409]
2. Sanocka D, kurpisz M. Reactive oxygen species and sperm cells. Reprod Biol Endocrinol 2004; 2: 12-19. [DOI:10.1186/1477-7827-2-12]
3. Agarwal A, saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil and Steril 2003; 79: 829-843. [DOI:10.1016/S0015-0282(02)04948-8]
4. Girotti MJ, khao N, Melellen BA. Early measurement of systemic lipid peroxidation products in plasma of major blunt trauma patients. J Trauma 1991; 31: 32-35. [DOI:10.1097/00005373-199101000-00007]
5. Zini A, de Lamirande E, Gagnon C. Reactive oxygen species in semen of infertile patients: levels of superoxide dismutase and catalase-like activities in seminal plasma and spermatozoa. Int J Andro 1993; 16: 183-188. [DOI:10.1111/j.1365-2605.1993.tb01177.x]
6. Thiele JJ, Friesleben HJ, Fuchs J, Ochsendorf FR. Ascorbic acid and urate in human seminal plasma: determination and interrelationships with chemiluminescence in washed semen. Human Reproduction 1995; 10: 110-115. [DOI:10.1093/humrep/10.1.110]
7. Tavilani H, Doosti M, Saeidi H. Malondialdehyde levels in sperm and seminal plasma of asthenozoospermic and its relationship with semen parameters. Clinica Chem Acta 2005; 365: 199-203. [DOI:10.1016/j.cccn.2005.01.017]
8. Koca Y, O¨zdal O¨ L, C¸elik M, U¨nal S, Balaban N. Antioxidant activity of seminal plasma in fertile and infertile men. Archives of Andrology 2003; 49: 355-359. [DOI:10.1080/01485010390219728]
9. Song GJ, Norkus EP, Lewis V. Relationship between seminal ascorbic acid and sperm DNA integrity in infertile men. International Journal of Andrology 2006; 29; 569-575. [DOI:10.1111/j.1365-2605.2006.00700.x]
10. World Health Organization WHO: Laboratory Manual for the examination of Human semen and semen- cervical Mucus interaction. 4th ed. Cambridge, UK, Cambridge University press, 1999.
11. Yagi K. Assay for blood plasma or serum. Methods Enzymol 1984; 105: 328-331. [DOI:10.1016/S0076-6879(84)05042-4]
12. Lee Bl, Chua SC, Ong HY, Ong CN. HPLC Method for routine determination of vitamins A and B- carotene in plasma. Journal of Chromato 1992; 581: 41- 47. [DOI:10.1016/0378-4347(92)80445-V]
13. Natelson S. Determination of ascorbic acid using 2,4 dinitrophneyl hydrazine. In, "Technique of clinical chemistry." C C. Thomas, Spring field, Illinois, 1971.
14. Landat A, Karine L, Guechot J, Palluet AM. Values of sperm thiobarbituric acid- reactive substances in fertile men. Clin Chem Acta 2002; 325: 113-115. [DOI:10.1016/S0009-8981(02)00289-9]
15. Shang XJ, Lik Y, Chen YG, Huang YF: Analysis of lipid peroxidative levels in seminal plasma of in fertile men by HPLC. Arch Androl 2004; 50: 411-416. [DOI:10.1080/01485010490484138]
16. Mostafa T, Anis TH, Ghazi S, El-Nashar AR, Imam H, Osman IA. Reactive oxygen species and antioxidants relationship in the internal spermatic vein blood of infertile men with varicocele. Asian J Androl 2006; 8: 451-454. [DOI:10.1111/j.1745-7262.2006.00172.x]
17. Fraga CG, Motchnik PA, Shigenaga MK, Helbock HJ, Jacob RA, Ames BN. Ascorbic acid protects against endogenous oxidative DNA damage in human sperm. Proceedings of the National Academy of Sciences of the United States of America 1991; 88: 11003-11006. [DOI:10.1073/pnas.88.24.11003]
18. Lewis SE, Sterling ES, Young IS, Thompson W. Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men. Fertil and Steril 1997; 67: 142-147. [DOI:10.1016/S0015-0282(97)81871-7]
19. Buettner GR. The pecking order of fl'ee radicals and antioxidants, lipid peroxidation, alpha-tocopherol and ascorbate. Arch Biochem Biophys 1993; 300: 535-543. [DOI:10.1006/abbi.1993.1074]
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.