دوره 6، شماره 3 - ( 4-1387 )                   جلد 6 شماره 3 صفحات 0-101 | برگشت به فهرست نسخه ها

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Babolhavaeji H, Mousavi Bahar S H, Anvari N, Abed F, Roshanpour A. The study of detecting sperm in testis biopsy in men with severe oligospermia and azoospermia by two methods of wet prep cytologic and classic histopathologic. IJRM. 2008; 6 (3) :101-0
URL: http://ijrm.ssu.ac.ir/article-1-104-fa.html
The study of detecting sperm in testis biopsy in men with severe oligospermia and azoospermia by two methods of wet prep cytologic and classic histopathologic. International Journal of Reproductive BioMedicine. 1387; 6 (3) :0-101

URL: http://ijrm.ssu.ac.ir/article-1-104-fa.html


چکیده:   (1747 مشاهده)
Background: Many azoospermic patients with non obstructive azoospermia (NOA) are candidate for testicular sperm extraction (TESE) and in vitro fertilization. Because sperm might be present in some but not all parts of the testes of such men, multiple sampling of testicular tissue are usually necessary to increase the probability of sperm finding. Sperm finding can be done by two methods: 1) classic histopathology and 2) wet smear.
Objective: Comparative study of pathology and wet smear methods for discovering sperm in testis biopsy of azoospermic men.
Materials and Methods: We prospectively studied 67 consecutive infertile men who referred to Fatemieh Hospital, Hamedan, Iran between April 2002 and September 2004. All patients were either azoospermic or severely oligozoospermic. They underwent intraoperative wet prep cytological examinations of testis biopsy material and then specimens were permanently fixed for pathologic examination too.
Results: Among the 67 testes that underwent wet prep cytological examination, 44 (65.7%) were positive and 23 (34.3%) had no sperm in their wet smear. On the permanent pathologic sections, 19 (28.4%) were positive and 48 (71.6%) cases were with no sperm in their sections. Among all the individuals 18 (26.8%) were negative in both studies, while 14 (20.8%) had minimum 1 sperm in their smears in both examinations. The positive cases in wet prep cytological examination were significantly more than the cases in the permanent histopathologic sections (p-value=0.000).
Conclusion: It seems that wet prep cytological examination is more reliable than permanent histopathologic sections in detecting sperm in testis biopsy of azoospermic men.
نوع مطالعه: Original Article |

فهرست منابع
1. Papadimas J, Papadopoulou F, Ioannidis S, Spanos E, Tarlatzis B, Bontis J, et al. Azoospermia: clinical, hormonal, and biochemical investigation. Arch Androl 1996; 37: 97-102. [DOI:10.3109/01485019608988509]
2. Mazzilli F, Rossi T, Delfino M, Sarandrea N, Dondero F. Azoospermia: incidence, and biochemical evaluation of seminal plasma by the differential pH method. Panminerva Med 2000; 42: 27-31.
3. Silber SJ, Nagy Z, Devroey P, Tournaye H, Van Steirteghem AC. Distribution of spermatogenesis in the testicles of azoospermic men: the presence or absence of spermatids in the testes of men with germinal. Hum Reprod 1997; 12: 2422-2428. [DOI:10.1093/humrep/12.11.2422]
4. Hauser R, Botchan A, Amit A, Yosef D Ben, Gamzu R, Paz G, et al. Multiple testicular sampling in non-obstructive azoospermia- is it necessary? Hum Reprod 1998; 13: 3081-3085. [DOI:10.1093/humrep/13.11.3081]
5. Hotchkiss RS. Testicular biopsy in the diagnosis and treatment of sterility in the male. Bull NY Acad Med 1942; 18: 600-605.
6. Charny CW. Testicular biopsy: its value in male infertility. J Am Med Assoc l940; l 15:1429-1432.
7. Silber SJ. Pregnancy caused by spermatozoa from vasa efferentia. Fertil Steril l988; 49: 373-375. [DOI:10.1016/S0015-0282(16)59733-7]
8. Coburn M, Wheeler TM, Lipshultz LI. Cytological examination of testis biopsy specimens. Fertil Steril AFS Annual Meeting Program Supplement 1986:122.
9. Oates R, DeLas Morenas A, Lipshultz LI. Clinical application of the testicular touch imprint. Fertil Steril AFS Annual Meeting Program Supplement 1989:17.
10. Magid MS. Cash KL, Goldstein M. The testicular biopsy in the evaluation of infertility. Semin Urol l990; 8: 5l-64.
11. Jow WW, Steckel J, Schlegel PN, Magid MS, Goldstein M. Motile sperm in human testis biopsy specimens. J Androl 1993; 14: 194-198.
12. Steckel J, Goldstein M. Value of intraoperative wet prep cytology in predicting final histology of testis biopsy. Fertile Steril AFS Annual Meeting Program Supplement 1991: 27.
13. Young WC. A study of the function of the epididymides. J Exp Biol 1931; 8:151-162.
14. Silber SJ, Ord T, Balmaceda J, Pati-izio P, Asch RH. Congenital absence of the vas deferens. The fertilizing capacity of human epididymal sperm. N Engl J Med 1990; 323: 1788-1792. [DOI:10.1056/NEJM199012273232602]
15. Cooper TO. In defense of a function for the human epididymis. Fertil Steril l990; 54: 965-975. [DOI:10.1016/S0015-0282(16)53988-0]
16. Orgebin-Crist MC, Danzo BJ, Cooper TO. Re-examination of the dependence of epididymal environment. J Reprod Fert Suppl l976; 24: 115-128.

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