Volume 7, Issue 3 (7-2009)
IJRM 2009, 7(3): 135-140 |
Back to browse issues page
Fattaneh Farifteh * 
1, Marzieh Mehrafza2 , Mona Oudi2 , Azadeh Eftekhari2 , Maryam Rahnavard2 , Khatereh Barjasteh2 , Ahmad Hossieni2
1, Marzieh Mehrafza2 , Mona Oudi2 , Azadeh Eftekhari2 , Maryam Rahnavard2 , Khatereh Barjasteh2 , Ahmad Hossieni2
1- Mehr Infertility Institute, Rasht, Iran , f.farifteh@yahoo.com
2- Mehr Infertility Institute, Rasht, Iran
2- Mehr Infertility Institute, Rasht, Iran
Abstract: (2557 Views)
Background: Embryo transfer (ET) has traditionally been performed two days after oocyte retrieval. Delaying transfer from day two to day three or four would allow for further development of the embryo might therefore optimize the selection of viable and best quality embryos for transfer and may be closer to the physiological time of the entry into the uterus than transfer on day two and might have a positive effect on pregnancy outcomes.
Objective: The study aimed to determine whether delayed transfer of embryos from 2 to 3 or 4 days after oocyte retrieval and the pregnancy rate in ICSI (intra cytoplasmic sperm injection) procedure.
Materials and Methods: In this deh1ive study we evaluated infertile couples who were referred to the Mehr Infertility Institute between 2006 and 2008 for ICSI according to the day of embryo transfer. We compared embryo quality pregnancy rate and implantation rate among day 2 3 or 4 of transfers. Pregnancy rate was confirmed by measurement of β-hCG in serum after 14 days. After data collection analysis was carried out with the t-test and chi squared tests by using the statistical software package SPSS.16.
Results: The overall clinical pregnancy rate (CPR) reported was 46.6%. The mean age of women and duration of infertility didn,t differ on the day of embryo transfer (p>0.05). Overall CPRs were not statistically different for day 2 (50.3%) day 3 (46.5%) and day 4 (34.8%) transfers respectively there were no significant differences in the age of transferred embryos between pregnant and nonpregnant women (p>0.05).
Conclusion: From the result of the present study there were no statistically significant differences in pregnancy rates according to the day of embryo transfer.
Objective: The study aimed to determine whether delayed transfer of embryos from 2 to 3 or 4 days after oocyte retrieval and the pregnancy rate in ICSI (intra cytoplasmic sperm injection) procedure.
Materials and Methods: In this deh1ive study we evaluated infertile couples who were referred to the Mehr Infertility Institute between 2006 and 2008 for ICSI according to the day of embryo transfer. We compared embryo quality pregnancy rate and implantation rate among day 2 3 or 4 of transfers. Pregnancy rate was confirmed by measurement of β-hCG in serum after 14 days. After data collection analysis was carried out with the t-test and chi squared tests by using the statistical software package SPSS.16.
Results: The overall clinical pregnancy rate (CPR) reported was 46.6%. The mean age of women and duration of infertility didn,t differ on the day of embryo transfer (p>0.05). Overall CPRs were not statistically different for day 2 (50.3%) day 3 (46.5%) and day 4 (34.8%) transfers respectively there were no significant differences in the age of transferred embryos between pregnant and nonpregnant women (p>0.05).
Conclusion: From the result of the present study there were no statistically significant differences in pregnancy rates according to the day of embryo transfer.
Type of Study: Original Article |
References
1. Coskun S, Jaroudi K, Sieck U, Alsufyan H, Alhassan S, Alkabra M. Pronuclear embryo transfer on day 1 versus cleavage stage embryo transfer on day 3: a prospective randomized trial. Fertility & Sterility 2002; 78: 45. [DOI:10.1016/S0015-0282(02)03497-0]
2. Laverge H, De Sutter P, Van der Elst J, Dhont M. A prospective randomized study comparing day 2 and day 3 embryo transfer in human IVF. Hum Reprod 2001; 16: 476-480. [DOI:10.1093/humrep/16.3.476]
3. Harper MJ. Gamete and zygote transport. In: Knobil E, Neill JD (eds) The physiology of reproduction 1994; 1: 123-188.
4. Braude P, Bolton V, Moore S. Human gene expression first occurs between the four and eight cell stage of preimplantation development. Nature 1988; 332:459-461. [DOI:10.1038/332459a0]
5. de los Santos MJ, Mercader A, Galán A, Albert C, Romero JL, Pellicer A. Implantation rates after two, three, or five days of embryo culture. Placenta 2003; 24: 13-19. [DOI:10.1016/S0143-4004(03)00172-3]
6. Ertzeid G, Dale PO, Tanbo T, Storeng R, Kjekshus E, Abyholm T. Clinical outcome of day 2 versus day 3 embryo transfer using serum free culture media: A prospective randomized study. J Asist Reprod Genet 1999; 16: 529-534. [DOI:10.1023/A:1020597104326]
7. Huisman GJ, Fauser BCJM, Eijkemans MJC, Pieters MHEC. Implantation rates after in vitro fertilization and transfer of a maximum of two embryos that have undergone three to five days of culture. Fertil Steril 2000; 73: 117-122. [DOI:10.1016/S0015-0282(99)00458-6]
8. Oatway C, Gunby J, Daya S. Day three versus day two embryo transfer following in vitro fertilization or intracytoplasmic sperm injection. Cochrane Database Syst Rev. 2004; 2: CD004378 [DOI:10.1002/14651858.CD004378]
9. van Os HC, Alberda AT, Janssen-Caspers HA, Leerentveld RA, Scholtes MC, Zeilmaker GH.The influence of the interval between in vitro fertilization and embryo transfer and some other variables on treatment outcome. Fertil Steril 1989; 51: 360-362. [DOI:10.1016/S0015-0282(16)60508-3]
10. Baruffi RL, Mauri AL, Peterson C, Felipe V, Franco Junior JG. Day 2 vs. day 3 embryo transfer after intracytoplasmic sperm injection. A prospective randomized study. J Reprod Med 2003; 48: 631-634.
11. Ashrafi M, Kiani K, Mirzaagha E, Shabani F. The Pregnancy Outcomes of Day 2 versus Day 3 Embryo Transfer: A Cross-Sectional Study. Intel J of Fertil & Steril 2007; 1: 55-62.
12. Goto Y, Kanzaki H, Nakayama T, Takabatake K, Himeno T, Mori T,et al. Relationship between the day of embryo transfer and the outcome in human in vitro fertilization and embryo transfer. J Assist Reprod Genet 1994; 11:401-404. [DOI:10.1007/BF02211726]
13. Lundin K, Bergh C, Hardarson T. Early embryo cleavage is a strong indicator of embryo quality in human IVF. Hum Reprod 2001; 16: 2652-2657. [DOI:10.1093/humrep/16.12.2652]
14. Carrillo AJ, Lane B, Pridman DD, Risch PP, Pool TB, Silverman IH, et al. Improved clinical outcomes for in vitro fertilization with delay of embryo transfer from 48 to 72 hours after oocyte retrieval: use of glucose- and phosphate-free media. Fertil Steril 1998; 69: 329-334. [DOI:10.1016/S0015-0282(97)00499-8]
15. Dawson KJ, Conaghan J, Ostera GR, Winston RM, Hardy K. Delaying transfer to the third day post-insemination, to select non-arrested embryos, increases development to the fetal heart stage. Hum Reprod 1995; 10: 177-182. [DOI:10.1093/humrep/10.1.177]
16. Ertzeid G, Dale PO, Tanbo T, Storeng R, Kjekshus E, Abyholm T. Clinical outcome of day 2 versus day 3 embryo transfer using serum-free culture media: a prospective randomized study. J Assist Reprod Genet 1999; 16: 529-534. [DOI:10.1023/A:1020597104326]
17. Aboulghar MM, Aboulghar MA, Mansour RT, Serour GI, Amin YM, Abou-Setta AM. Pregnancy rate is not improved by delaying embryo transfer from days 2 to 3. Eur J Obstet Gynecol Reprod Biol 2003; 107: 176-179. [DOI:10.1016/S0301-2115(02)00400-1]
18. Bahceci M, Ulug U, Ciray HN, Akman MA, Erden HF. Efficiency of changing the embryo transfer time from day 3 to day 2 among women with poor ovarian response: a prospective randomized trial. Fertil Steril 2006; 86: 81-85. [DOI:10.1016/j.fertnstert.2005.12.029]
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |