Volume 13, Issue 12 (12-2015)                   IJRM 2015, 13(12): 749-754 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Eftekhar M, Pourmasumi S, Sabeti P, Aflatoonian A, Sheikhha M H. Mycobacterium tuberculosis infection in women with unexplained infertility. IJRM 2015; 13 (12) :749-754
URL: http://ijrm.ir/article-1-616-en.html
Mycobacterium tuberculosis infection in women with unexplained infertility
Maryam Eftekhar1 , Soheila Pourmasumi * 2, Parvin Sabeti1 , Abbas Aflatoonian1 , Mohammad Hasan Sheikhha1
1- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , spourmasumi@yahoo.com
Abstract:   (2893 Views)
Background: Genital tuberculosis (GTB) is an important cause of female infertility, especially in developing countries. The positive results of polymerase chain reaction (PCR) in endometrial GTB in the absence of tubal damage raise the possibility of the detection of sub-clinical or latent disease, with doubtful benefits of treatment. Objective: To evaluate the mycobacterium tuberculosis infection in endometrial biopsy samples collected from unexplained infertile women attending Yazd Research and Clinical Center for Infertility by using PCR techniques. Materials and Methods: In this cross sectional study, 144 infertile women with unexplained infertility aged 20-35 years old and normal Histro-saplango graphy findings were enrolled. Endometrial biopsy samples from each participant were tested for mycobacterium tuberculosis detecting by PCR. In 93 patients, peritoneal fluid was also taken for culture and PCR. Results: The PCR results of endometrial specimens were negative in all cases, demonstrating that there was no GTB infection among our patients. Conclusion: Our results showed that GTB could not be considered as a major problem in women with unexplained infertility. Although, studies have indicated that PCR is a useful method in diagnosing early GTB disease in infertile women with no demonstrable evidence of tubal or endometrial involvement.
Keywords: Genital mycobacterium tuberculosis, Polymerase chain reaction, Unexplained infertility.
Full-Text [PDF 399 kb]   (926 Downloads) |   |   Full-Text (HTML)  (318 Views)  
Type of Study: Original Article |
References
1. Makar RS, Toth TL. The evaluation of infertility. Am J Clin Pathol 2002; 117: S95-103. [DOI:10.1309/W8LJ-K377-DHRA-CP0B]
2. Huang HF, Liu AX. Research progress of the diseases associated infertility and assisted reproductive technology. Zhejiang Da Xue Xue Bao Yi Xue Ban 2007; 36: 417-423.
3. Esmaeilzadeh S, Delavar MA, Zeinalzadeh M, Mir MR. Epidemiology of infertility: a population-based study in Babol, Iran. Women Health 2012; 52: 744-754. [DOI:10.1080/03630242.2012.728191]
4. Bhattacharya S, Porter M, Amalraj E, Templeton A, Hamilton M, Lee A, et al. The epidemiology of infertility in the North East of Scotland. Hum Reprod 2009; 24: 3096-3107. [DOI:10.1093/humrep/dep287]
5. Wilkes S, Chinn DJ, Murdoch A, Rubin G. Epidemiology and management of infertility: a population-based study in UK primary care. Fam Pract 2009; 26: 269-274. [DOI:10.1093/fampra/cmp029]
6. Schmidt L. Infertility and assisted reproduction in Denmark. Epidemiology and psychosocial consequences. Dan Med Bull 2006; 53: 390-417.
7. Marzieh N. Epidemiology of infertility in the west of Tehran in 2000. J Am Med Womens Assoc 2002; 57: 219.
8. Templeton A. Infertility and the establishment of pregnancy–overview. Br Med Bull 2000; 56: 577-587. [DOI:10.1258/0007142001903283]
9. Pellati D, Mylonakis I, Bertoloni G, Fiore C, Andrisani A, Ambrosini G, et al. Genital tract infections and infertility. Eur J Obstet Gynecol Reprod Biol, 2008; 140: 3-11. [DOI:10.1016/j.ejogrb.2008.03.009]
10. Stanislavov R, Chernev T, Nikolova V, Markova M. Asymptomatic infections of the genital tract and infertility. Akush Ginekol (Sofiia) 2000; 39: 18-20.
11. Shukla Upadhyaya K, Karunagoda K, Dei LP. Infertility caused by tubal blockage: An ayurvedic appraisal. AYU 2010; 31: 159-166. [DOI:10.4103/0974-8520.72378]
12. Mahutte NG, Arici A. New advances in the understanding of endometriosis related infertility. J Reprod Immunol 2002; 55: 73-83. [DOI:10.1016/S0165-0378(01)00130-9]
13. Bender N, Herrmann B, Andersen B, Hocking JS, van Bergen J, Morgan J, et al. Chlamydia infection, pelvic inflammatory disease, ectopic pregnancy and infertility: cross-national study. Sex Transm Infect 2011; 87: 601-608. [DOI:10.1136/sextrans-2011-050205]
14. Du YB, Gao MZ, Shi Y, Sun ZG, Wang J. Endocrine and inflammatory factors and endometriosis-associated infertility in assisted reproduction techniques. Arch Gynecol Obstet 2013; 287: 123-130. [DOI:10.1007/s00404-012-2567-0]
15. Unuane D, Tournaye H, Velkeniers B, Poppe K. Endocrine disorders & female infertility. Best Pract Res Clin Endocrinol Metab 2011; 25: 861-873. [DOI:10.1016/j.beem.2011.08.001]
16. Belaisch-Allart J. Treatment options for age-related infertility. Rev Prat 2010; 60: 819-823.
17. Szewczuk E. Age related infertility: a tale of two technologies. Sociol Health Illn 2012; 34: 429-443. [DOI:10.1111/j.1467-9566.2011.01382.x]
18. Kamath MS, Bhattacharya S. Demographics of infertility and management of unexplained infertility. Best Pract Res Clin Obstet Gynaecol 2012; 26: 729-738. [DOI:10.1016/j.bpobgyn.2012.08.001]
19. Aisenberg Romano G, Ravid H, Zaig I, Schreiber S, Azem F, Shachar I, et al. The psychological profile and affective response of women diagnosed with unexplained infertility undergoing in vitro fertilization. Arch Womens Ment Health 2012; 15: 403-411. [DOI:10.1007/s00737-012-0299-6]
20. Ali AA, Abdallah TM. Clinical presentation and epidemiology of female genital tuberculosis in eastern Sudan. Int J Gynaecol Obstet 2012; 118: 236-238. [DOI:10.1016/j.ijgo.2012.04.005]
21. Shaheen R, Subhan F, Tahir F. Epidemiology of genital tuberculosis in infertile population. J Pak Med Assoc 2006; 56: 306-309.
22. Singh N, Sumana G, Mittal S. Genital tuberculosis: a leading cause for infertility in women seeking assisted conception in North India. Arch Gynecol Obstet 2008; 278: 325-327. [DOI:10.1007/s00404-008-0590-y]
23. Baum SE, Dooley DP, Wright J, Kost ER, Storey DF. Diagnosis of culture-negative female genital tract tuberculosis with peritoneal involvement by polymerase chain reaction. J Reprod Med 2001; 46: 929-932.
24. Bhanu NV, Singh UB, Chakraborty M, Suresh N, Arora J, Rana T, et al. Improved diagnostic value of PCR in the diagnosis of female genital tuberculosis leading to infertility. J Med Microbiol 2005; 54: 927-931. [DOI:10.1099/jmm.0.45943-0]
25. Thangappah RB, Paramasivan CN, Narayanan S. Evaluating PCR, culture & histopathology in the diagnosis of female genital tuberculosis. Indian J Med Res 2011; 134: 40-46.
26. Qureshi RN, Samad S, Hamid R, Lakha SF. Female genital tuberculosis revisited. J Pak Med Assoc 2001; 51: 16-18.
27. Tripathy SN, Tripathy SN. Infertility and pregnancy outcome in female genital tuberculosis. Int J Gynaecol Obstet 2002; 76: 159-163. [DOI:10.1016/S0020-7292(01)00525-2]
28. Mani R, Nayak S, Kagal A, Deshpande S, Dandge N, Bharadwaj R. Tuberculous endometritis in infertility: a bacteriological and histopathological study. Indian J Tuberc 50: 161-162.
29. Biswas D, Agrawal M, Ali A, Bhargava R. Anti ES-31/41 antibodies: A potential tool in the serodiagnosis of extra-pulmonary Tuberculosis. Indian J Tuberc 2003; 50: 203-207.
30. Abebe M, Lakew M, Kidane D, Lakew Z, Kiros K, Harboe M. Female genital tuberculosis in Ethiopia. Int J Gynaecol Obstet 2004; 84: 241-246. [DOI:10.1016/j.ijgo.2003.11.002]
31. Hassoun A, Jacquette G, Huang A, Anderson A, Smith MA. Female genital tuberculosis: uncommon presentation of tuberculosis in the United States. Am J Med 2005; 118: 1295-1296. [DOI:10.1016/j.amjmed.2005.06.025]
32. Sharma JB, Roy KK, Pushparaj M, Kumar S. Hysteroscopic findings in women with primary and secondary infertility due to genital tuberculosis. Int J Gynaecol Obstet 2009; 104: 49-52. [DOI:10.1016/j.ijgo.2008.08.019]
33. Whitehill K, Yong PJ, Williams C. Clinical predictors of endometriosis in the infertility population: is there a better way to determine who needs a laparoscopy? J Obstet Gynaecol Can 2012; 34: 552-557. [DOI:10.1016/S1701-2163(16)35271-9]
34. Mondal SK, Dutta T. A ten year clinicopathological study of female genital tuberculosis and impact on fertility. JNMA J Nepal Med Assoc 2009; 48: 52-57.
35. Banerjee A, Prateek S, Malik S, Dhingra D. Genital tuberculosis in adolescent girls from low socioeconomic status with acute ectopic pregnancy presenting at a tertiary care hospital in urban Northern India: are we missing an opportunity to treat? Arch Gynecol Obstet 2012; 286: 1477-1482. [DOI:10.1007/s00404-012-2487-z]
36. Pešut D, Stojšić J. Female genital tuberculosis: A disease seen again in Europe. Vojnosanit Pregl 2007; 64: 855-858. [DOI:10.2298/VSP0712855P]
37. Metanat M, Sharifi-Mood B, Alavi-Naini R, Aminianfar M. The epidemiology of tuberculosis in recent years: Reviewing the status in south-eastern Iran. ZJRMS 2012; 13: 1-7.
38. Hatami M. Tuberculosis of the female genital tract in Iran. Arch Iranian Med 2005; 8: 32-35.
39. Ghosh SK, Mondal R. Quick diagnosis of female genital tuberculosis using multiplex fast polymerase chain reaction in Southern Assam, India. Int J Gynaecol Obstet 2012; 118: 72-73. [DOI:10.1016/j.ijgo.2012.02.006]
40. Rana T, Singh UB, Kulshrestha V, Kaushik A, Porwal C, Agarwal N, et al. Utility of reverse transcriptase PCR and DNA-PCR in the diagnosis of female genital tuberculosis. J Med Microbiol 2011; 60: 486-491. [DOI:10.1099/jmm.0.025080-0]
41. Baxi A, Neema H, Kaushal M, Sahu P, Baxi D. Genital tuberculosis in infertile women: Assessment of endometrial TB PCR results with laparoscopic and hysteroscopic features. J Obstet Gynaecol India 2011; 61: 301-306. [DOI:10.1007/s13224-011-0046-3]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb