Volume 2, Issue 1 (7-2004)                   IJRM 2004, 2(1): 34-39 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Zarghami N, Khosrowbeygi A. Evaluation of Lipid Peroxidation as an Indirect Measure of Oxidative Stress in Seminal Plasma. IJRM 2004; 2 (1) :34-39
URL: http://ijrm.ir/article-1-9-en.html
Evaluation of Lipid Peroxidation as an Indirect Measure of Oxidative Stress in Seminal Plasma
Nosratollah Zarghami * , Ali Khosrowbeygi
Abstract:   (2978 Views)
Background: It has been proposed that oxidative stress plays an important role in male infertility. The aims of this study were to compare seminal plasma levels of 15-F2t-isoprostane (8-iso-PGF2?), malondialdehyde (MDA), and total (sum of free and bound) homocysteine (tHcy) in normozoospermic vs. asthenozoospermic men, and to examine the relationships between tHcy and lipid peroxidation products. Materials and Methods: The study was a case-control study with a simple random sampling. The case group consisted of 15 asthenozoospermic males. This group was compared with 15 normozoospermic men. Seminal plasma levels of 15-F2t-isoprostane and tHcy were measured using commercially available enzyme immunoassay (EIA) kits. MDA levels were determined by the thiobarbituric acid (TBA) assay. The Mann-Whitney U test was used to compare two groups. Coefficients of correlation were calculated using Spearman’s correlation analysis. All hypothesis tests were two-tailed with statistical significance assessed at the p value <0.05 level. Results: MDA levels were lower in asthenozoospermic subjects than in control subjects (0.72±0.06 µM vs. 0.40±0.06 µM; p<0.05). No differences were seen in 15-F2t-isoprostane levels in asthenozoospermic subjects and controls (65.00±3.20 pg/ml vs. 58.17±4.12 pg/ml; p>0.05). Interestingly, tHcy levels were slightly higher in asthenozoospermic subjects than in controls (6.18±1.17 µM vs. 4.8±0.52µM). Sperm motility was inversely correlated with seminal plasma 15-F2t-isoprostane and MDA levels, respectively (p<0.05). Conclusion: Seminal plasma levels of 15-F2t-isoprostane and tHcy showed no significant differences between normozoospermic and asthenozoospermic men. Sperm motility correlated inversely with seminal plasma levels of 15-F2t-isoprostane and MDA. No relationship was found between tHcy and lipid peroxidation. However, higher sample size is required to confirm these findings.
Keywords: Seminal Plasma, 15-F2t-Isoprostane, Malondialdehyde, Homocysteine, Lipid Peroxidation, Asthenozoospermia, Normozoospermia
Full-Text [PDF 174 kb]   (931 Downloads) |   |   Full-Text (HTML)  (401 Views)  
Type of Study: Original Article |
References
1. Agarwal A., and Saleh R.A. (2002) Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin North Am 29:817-827. [DOI:10.1016/S0094-0143(02)00081-2]
2. Agarwal A., Saleh R.A., and Bedaiwy M.A. (2003) Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 79:829-843. [DOI:10.1016/S0015-0282(02)04948-8]
3. Antoine L., Karine L., Jérôme G., and Anne-Marie P. (2002) Values of sperm thiobarbituric acid-reactive substance in fertile men. Clinica Chimica Acta 325:113-115. [DOI:10.1016/S0009-8981(02)00289-9]
4. Dandekar S.P., Nadkarni G.D., Kulkarni V.S., and Punekar S. (2002) Lipid peroxidation and antioxidant enzymes in male infertility. J Postgrad Med 48:186-189.
5. Fraczek M., Szkutnik D., Sanocka D., and Kurpisz M. (2001) Peroxidation components of sperm lipid membranes in male infertility. Ginekologia Polska 72:73-79.
6. Gagnon C., and de Lamirande E. (1999) Extrinsic factors affecting sperm motility: immunological and infectious factors and reactive oxygen species. In: Male sterility and motility disorders, (ed. S. Hamaman et al.), 37-44. Springer-Verlag, New York. [DOI:10.1007/978-1-4612-1522-6_3]
7. Gomez E., Irvine D.S., and Aitken R.J. (1998) Evaluation of a spectrophotometric assay for the measurement of malondialdehyde and 4-hydroxyalkenals in human spermatozoa: relationships with semen quality and sperm function. Int J Androl 21:81-94. [DOI:10.1046/j.1365-2605.1998.00106.x] [PMID]
8. Hull M.G., Glazener C.M., Conway D.I., Foster P.A., Hinton R.A., Coulson C. et al. (1995) Population study of causes, treatment and outcome of infertility. Br Med J 291:1693-1697. [DOI:10.1136/bmj.291.6510.1693] [PMID] [PMCID]
9. Ichikawa T., Oeda T., Ohmori H., and Schill W.B. (1999) Reactive oxygen species influence the acrosome reaction but not acrosin activity in human spermatozoa. Int J Androl 22:37-42. [DOI:10.1046/j.1365-2605.1999.00145.x] [PMID]
10. Jacobsen, D.W. (2000) Hyperhomocysteinemia and oxidative stress: time for a reality check? Arterioscler Thromb Vasc Biol 20:1182-1184. [DOI:10.1161/01.ATV.20.5.1182] [PMID]
11. Keskes-Ammar L., Feki-Chakroun N., Rebai T., Sahnoun Z., Ghozzi H., Hammami S. et al. (2003) Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Arch Androl 49:83-94. [DOI:10.1080/01485010390129269] [PMID]
12. Lawson J.A., Rokach J., and FitzGerald G.A. (1999) Isoprostanes: formation, analysis and use as indices of lipid peroxidation in vivo. J Biol Chem 274:24441-24444. [DOI:10.1074/jbc.274.35.24441] [PMID]
13. Meagher E.A., and Fitz Gerald G.A. (2000) Indices of lipid peroxidation in vivo: strengths and limitations. Free Radic Biol Med 28:1745-1750. [DOI:10.1016/S0891-5849(00)00232-X]
14. Nakamura H., Kimura T., Nakajima A., Shimoya K., Takemura M., Hashimoto K. et al. (2002) Detection of oxidative stress in seminal plasma and fractionated sperm from subfertile male patients. Eur J Obstet Gynecol Reprod Biol 105:155-160. [DOI:10.1016/S0301-2115(02)00194-X]
15. Pasqualotto F.F., Sharma R.K., Nelson D.R., Thomas A.J. J.r., and Agarwal A. (2000) Relationship between oidative stress, semen characteristics, and clinical diagnosis in men undergoing infertility investigation. Fertil Steril 73:459-464. [DOI:10.1016/S0015-0282(99)00567-1]
16. Perna A.F., Ingrosso D., and De Santo N.G. (2003) Homocysteine and oxidative stress. Amino Acids 25:409-417. [DOI:10.1007/s00726-003-0026-8] [PMID]
17. Powers R.W., Majors A.K., Lykins D.L., Sims C.J., Lain K.Y., and Roberts J.M. (2002) Plasma homocysteine and malondialdehyde are correlated in an age- and gender-specific manner. Metabolism 51:1433-1438. [DOI:10.1053/meta.2002.35587] [PMID]
18. Pratico D., Lawson J.A., Rokach J., and FitzGerald G.A. (2001) The isoprostanes in biology and medicine. Trends Endocrinol Metab 12:243-247. [DOI:10.1016/S1043-2760(01)00411-8]
19. Rhemrev J.P., Vermeiden J.P., Haenen G.R., De Bruijne J.J., Rekers-Mombarg L.T., and Bast A. (2001) Progressively motile human spermatozoa are well protected against in vitro lipid peroxidation imposed by induced oxidative stress. Andrologia 33:151-158. [DOI:10.1046/j.1439-0272.2001.00430.x] [PMID]
20. Roberts L.J., and Morrow J.D. (2000) Measurement of F(2)-isoprostanes as an index of oxidative stress in vivo. Free Radic Biol Med 28:505-513. [DOI:10.1016/S0891-5849(99)00264-6]
21. Roberts L.J., and Morrow J.D. (2002) Products of the isoprostane pathway: unique bioactive compounds and markers of lipid peroxidation. Cell Mol Life Sci 59:808-820. [DOI:10.1007/s00018-002-8469-8] [PMID]
22. Sanocka D., and Kurpisz M. (2004) Reactive oxygen species and sperm cells. Reprod Biol Endocrinol 2:12. [DOI:10.1186/1477-7827-2-12] [PMID] [PMCID]
23. Suleiman S.A., Ali M.E., Zaki Z.M., el-Malik E.M., and Nasr M.A. (1996) Lipid peroxidation and human sperm motility: protective role of vitamin E. J Androl 17:530-537.
24. Voutilainen S., Morrow J.D., Roberts L.J., Alfthan G., Alho H., Nyyssonen K., and Salonen J.T. (1999) Enhanced in vivo lipid peroxidation at elevated plasma total homocysteine levels. Arterioscler Thromb Vasc Biol19:1263-1266. [DOI:10.1161/01.ATV.19.5.1263] [PMID]
25. World Health Organization. (1999) WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interactin. 3rd ed Cambridge University Press, Cambridge, UK.
26. Zappacosta B., Mordente A., Persichilli S., Minucci A., Carlino P., Martorana G.E. et al. (2001) Is homocysteine a pro-oxidant? Free Radic Res 35:499-505. [DOI:10.1080/10715760100301511] [PMID]
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb