International Journal of
Reproductive Biomedicine
Thu, Apr 3, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 248-248 |
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Moudi B, Heidari Z, Asemi-Rad A, Mahmoudzadeh-Sagheb H, Sheibak N, Ghasemi M et al . P-81 Cannabinoid receptor type-1 (CB1) and its correlation with CB1 gene polymorphism-1359G/A in ectopic pregnancy compared to the control group. IJRM 2021; 19 (5) :248-248
URL: http://ijrm.ir/article-1-2887-en.html
URL: http://ijrm.ir/article-1-2887-en.html
Moudi B * 
1, Heidari Z2 , Asemi-Rad A3 , Mahmoudzadeh-Sagheb H4 , Sheibak N5 , Ghasemi M6 , Eslami S7
1, Heidari Z2 , Asemi-Rad A3 , Mahmoudzadeh-Sagheb H4 , Sheibak N5 , Ghasemi M6 , Eslami S7
1- Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran. , bita.moudi@yahoo.com
2- Department of Histology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
3- Department of Anatomical Sciences, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
4- Cellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran.
5- Moloud Infertility Center, Ali Ibn Abitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran.
6- Pregnancy Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
7- Department of Obstetric and Gynecology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
2- Department of Histology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
3- Department of Anatomical Sciences, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
4- Cellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran.
5- Moloud Infertility Center, Ali Ibn Abitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran.
6- Pregnancy Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
7- Department of Obstetric and Gynecology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Abstract: (242 Views)
Background: Ectopic pregnancy (EP) is one of the most important causes of maternal mortality. Novel information could help to identify the signaling pathway and mechanism of fetal transfer disruption and its potential use in the diagnosis and prevention of this disorder.
Objective: This study aimed to evaluate the immunohistochemical expression of the cannabinoid receptor type 1 (CB1) and its association with CB1 -1359G/A gene polymorphism (rs1049353) in the fallopian tubes in EP compared to controls.
Materials and Methods: In this case control study, 100 women with EP (cases) and 100 women that underwent abdominal surgery due to the hysterectomy or uterine tubal ligation (healthy controls) were included. Genotyping of CB1-1359G/A polymorphism, tissue expression of CB1 at the protein and mRNA levels were studied using restriction fragment polymorphism, immunohistochemical (IHC) method, and quantitative real-time polymerase chain reaction (qRT-PCR) analysis.
Results: Genotyping showed that in EP, the frequency of AA, AA+AG genotypes, and A allele was significantly higher than healthy control subjects (p = 0.001). Also, patients with EP had significantly increased IHC expression of CB-1 compared to the control samples (p = 0.016). Patients with AA and AG genotypes had a significantly higher IHC expression of CB-1 compared to the GG genotype. Quantitative real-time PCR analysis showed that patients with EP had significantly increased expression of CB-1 compared to the control samples (p < 0.001). Patients with AA and AG genotypes had higher significant mRNA expression of CB-1 compared to the GG genotype.
Conclusion: Based on molecular and cellular analysis of CB1, the frequency of an allele and expression of CB1 were higher in patients with ectopic tubal pregnancy. Identifying the causes of the EP is essential to find effective methods of prevention and treatment of EP. CB1 is likely to be effective in creating innate immunity in humans and can affect the process of EP in the fallopian tube. CB1 is also a pathological valuable factor in identifying the pathway of inflammation during ectopic implantation. However, it is not possible to claim that a single SNP could be the only cause of the EP. In other words, EP is a polygenic disease, but the possible effects of these genetic changes in EP remain unknown. More investigations are required to introduce a risk prediction tool for susceptibility to EP.
Objective: This study aimed to evaluate the immunohistochemical expression of the cannabinoid receptor type 1 (CB1) and its association with CB1 -1359G/A gene polymorphism (rs1049353) in the fallopian tubes in EP compared to controls.
Materials and Methods: In this case control study, 100 women with EP (cases) and 100 women that underwent abdominal surgery due to the hysterectomy or uterine tubal ligation (healthy controls) were included. Genotyping of CB1-1359G/A polymorphism, tissue expression of CB1 at the protein and mRNA levels were studied using restriction fragment polymorphism, immunohistochemical (IHC) method, and quantitative real-time polymerase chain reaction (qRT-PCR) analysis.
Results: Genotyping showed that in EP, the frequency of AA, AA+AG genotypes, and A allele was significantly higher than healthy control subjects (p = 0.001). Also, patients with EP had significantly increased IHC expression of CB-1 compared to the control samples (p = 0.016). Patients with AA and AG genotypes had a significantly higher IHC expression of CB-1 compared to the GG genotype. Quantitative real-time PCR analysis showed that patients with EP had significantly increased expression of CB-1 compared to the control samples (p < 0.001). Patients with AA and AG genotypes had higher significant mRNA expression of CB-1 compared to the GG genotype.
Conclusion: Based on molecular and cellular analysis of CB1, the frequency of an allele and expression of CB1 were higher in patients with ectopic tubal pregnancy. Identifying the causes of the EP is essential to find effective methods of prevention and treatment of EP. CB1 is likely to be effective in creating innate immunity in humans and can affect the process of EP in the fallopian tube. CB1 is also a pathological valuable factor in identifying the pathway of inflammation during ectopic implantation. However, it is not possible to claim that a single SNP could be the only cause of the EP. In other words, EP is a polygenic disease, but the possible effects of these genetic changes in EP remain unknown. More investigations are required to introduce a risk prediction tool for susceptibility to EP.
Type of Study: Congress Abstract |
Subject:
Fertility & Infertility
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