International Journal of
Reproductive Biomedicine
Sun, Apr 6, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 114-114 |
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Sabour M, Agha-Rahimi A, Dehghani Ashkezari M, Seifati SM, Kalantar SM, Anbari F et al . O-13 Evaluation the effect of human sperm incubation time in polyvinylpyrrolidone on sperm structure reactive oxyen species, acrosome reaction, and mitochondorial membrane potential. IJRM 2021; 19 (5) :114-114
URL: http://ijrm.ir/article-1-2945-en.html
URL: http://ijrm.ir/article-1-2945-en.html
Sabour M1 
, Agha-Rahimi A * 2, Dehghani Ashkezari M1 , Seifati SM1 , Kalantar SM3 , Anbari F4 , Nabi A4
, Agha-Rahimi A * 2, Dehghani Ashkezari M1 , Seifati SM1 , Kalantar SM3 , Anbari F4 , Nabi A4
1- Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.
2- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,63rahimi@gmail.com
3- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,
3- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Abstract: (237 Views)
Background: Polyvinylpyrrolidone (PVP) is a chemical used in intracytoplasmic sperm injection for sperm immobilization. In human sperm, PVP has been shown to damage sperm membranes, DNA integrity, mitochondrial membrane, and destroy axonal tubules and fibrous sheaths.
Objective: The aim of this study was to investigate the ideal time that sperm can be safely incubated in PVP with less possible damage.
Materials and Methods: Twenty-five normospermic samples were used. Sperm samples were prepared by swim-up method. Sperm samples incubated in 10% PVP at different time intervals (0, 15, 30, and 60 min). The effect of PVP was assessed on sperm structure, reactive oxyen species, acrosome reaction, Mitochondorial Membrane potential at different time intervals.
Results: Sperm parameters, DNA integrity and chromatin quality in 15, 30 and 60 min after incubation sperm with PVP were significantly changed compared to the 0 min. Moreover, in 30 and 60 min after incubation with PVP, above parameters were significantly changed compared to the 15 min. 60 min after incubation sperm with PVP, these parameters were significantly changed compared to the 30 min.
Conclusion: Sperm samples could be incubated with PVP for 15 min with less possible damage. While, prolonged incubation may damage the sperm parameters, DNA integrity and chromatin quality significantly.
Objective: The aim of this study was to investigate the ideal time that sperm can be safely incubated in PVP with less possible damage.
Materials and Methods: Twenty-five normospermic samples were used. Sperm samples were prepared by swim-up method. Sperm samples incubated in 10% PVP at different time intervals (0, 15, 30, and 60 min). The effect of PVP was assessed on sperm structure, reactive oxyen species, acrosome reaction, Mitochondorial Membrane potential at different time intervals.
Results: Sperm parameters, DNA integrity and chromatin quality in 15, 30 and 60 min after incubation sperm with PVP were significantly changed compared to the 0 min. Moreover, in 30 and 60 min after incubation with PVP, above parameters were significantly changed compared to the 15 min. 60 min after incubation sperm with PVP, these parameters were significantly changed compared to the 30 min.
Conclusion: Sperm samples could be incubated with PVP for 15 min with less possible damage. While, prolonged incubation may damage the sperm parameters, DNA integrity and chromatin quality significantly.
Type of Study: Congress Abstract |
Subject:
Pregnancy Health
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