International Journal of
Reproductive Biomedicine
Sun, Apr 6, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 151-151 |
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Hajimaqsoudi E, Darbeheshti F, Kalantar SM, Javaheri A, Mirabutalebi SHR, Sheikhha MH. O-41 Investigating the expressions of miRNA-125b and TP53 in endometriosis: Does it underlie cancer-like features of endometriosis?. IJRM 2021; 19 (5) :151-151
URL: http://ijrm.ir/article-1-2974-en.html
URL: http://ijrm.ir/article-1-2974-en.html
1- Department of Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences,Yazd, Iran.
2- Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences,Tehran, Iran. Breast Cancer Association (BrCA), Universal Scientific Education and Research Network (USERN), Tehran, Iran.
3- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi Universityof Medical Sciences, Yazd, Iran.
4- Department of Obstetrics and Gynecology, Faculty of Medicine, Shahid Sadoughi Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5- Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
6- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi Universityof Medical Sciences, Yazd, Iran. ,sheikhha@yahoo.com
2- Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences,Tehran, Iran. Breast Cancer Association (BrCA), Universal Scientific Education and Research Network (USERN), Tehran, Iran.
3- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi Universityof Medical Sciences, Yazd, Iran.
4- Department of Obstetrics and Gynecology, Faculty of Medicine, Shahid Sadoughi Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5- Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
6- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi Universityof Medical Sciences, Yazd, Iran. ,
Abstract: (248 Views)
Background: Endometriosis is generally considered as a benign condition, but there is a possibility for it to become cancerous. miR-125b was upregulated in both endometriotic tissues and serum samples of women with endometriosis but its potential targets in endometriosis are still not fully understood.
Objective: The role of miR-125b in the regulation of TP53 expression in endometriosis was tested with a bioinformatics approach. In addition, the expression of miR-125b and TP53 in both eutopic endometrium (Eu-p) and ectopic endometrium (Ec-p) in endometrium tissues of patients with endometriosis was compared to these in the normal endometrium tissues of controls (Normal).
Materials and Methods: In this case-control study, the eutopic and ectopic samples were collected from 20 patients who underwent laparoscopic surgery and the normal endometrium tissues were collected from 20 controls with no evidence of endometriosis. For bioinformatics approach a protein-protein interaction network was constructed based on co-expressed potential targets of miR-125b. Quantitative PCR technique was used for measurement of miR125b and TP53 expression.
Results: Our results showed that miR-125b was significantly overexpressed in Ec-p. In addition, there was a significant TP53 underexpression in both Ec-p and Eu-p samples compared with normal tissues.
Conclusion: There was a negative correlation between miR-125b and TP53. In addition we observed a noticeable decreased expression of TP53 in both Ec-p and Eu-p samples. These findings may be interpreted as the roles of miR-125b/TP53 axis in the pathogenesis of endometriosis. With the help of bioinformatics analyses we conclude that there is a possible role of miR-125b in cancer-like features of endometriosis.
Objective: The role of miR-125b in the regulation of TP53 expression in endometriosis was tested with a bioinformatics approach. In addition, the expression of miR-125b and TP53 in both eutopic endometrium (Eu-p) and ectopic endometrium (Ec-p) in endometrium tissues of patients with endometriosis was compared to these in the normal endometrium tissues of controls (Normal).
Materials and Methods: In this case-control study, the eutopic and ectopic samples were collected from 20 patients who underwent laparoscopic surgery and the normal endometrium tissues were collected from 20 controls with no evidence of endometriosis. For bioinformatics approach a protein-protein interaction network was constructed based on co-expressed potential targets of miR-125b. Quantitative PCR technique was used for measurement of miR125b and TP53 expression.
Results: Our results showed that miR-125b was significantly overexpressed in Ec-p. In addition, there was a significant TP53 underexpression in both Ec-p and Eu-p samples compared with normal tissues.
Conclusion: There was a negative correlation between miR-125b and TP53. In addition we observed a noticeable decreased expression of TP53 in both Ec-p and Eu-p samples. These findings may be interpreted as the roles of miR-125b/TP53 axis in the pathogenesis of endometriosis. With the help of bioinformatics analyses we conclude that there is a possible role of miR-125b in cancer-like features of endometriosis.
Type of Study: Congress Abstract |
Subject:
Pregnancy Health
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