International Journal of
Reproductive Biomedicine
Mon, Nov 18, 2024
[Archive]
Volume 12, Issue 4 (5-2014)
IJRM 2014, 12(4): 243-248 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Hafizi L, Gholizadeh M, Karimi M, Hosseini G, Mostafavi-Toroghi H, Haddadi M, et al . Effects of magnetized water on ovary, pre-implantation stage endometrial and fallopian tube epithelial cells in mice. IJRM 2014; 12 (4) :243-248
URL: http://ijrm.ir/article-1-531-en.html
URL: http://ijrm.ir/article-1-531-en.html
Leili Hafizi1 
, Mostafa Gholizadeh2 , Mohammad Karimi3 , Golkoo Hosseini * 4, Hesam Mostafavi-Toroghi5 , Mehdi Haddadi6 , Amin Rezaiean3 , Mahmoud Ebrahimi7 , Neda Emami Meibodi8
, Mostafa Gholizadeh2 , Mohammad Karimi3 , Golkoo Hosseini * 4, Hesam Mostafavi-Toroghi5 , Mehdi Haddadi6 , Amin Rezaiean3 , Mahmoud Ebrahimi7 , Neda Emami Meibodi8
1- Department of Obstetrics and Gynecology, Mashhad University of Medical Sciences, Mashhad, Iran
2- Department of Chemistry, Faculty of Basic Sciences, Ferdowsi University, Mashhad, Iran
3- Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- Department of Obstetrics and Gynecology, Mashhad University of Medical Sciences, Mashhad, Iran ,Golkoo.hoseini@gmail.com
5- Biochemistry of Nutrition Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6- Department of Microbiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
7- Cardiovascular Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
8- Department of Biomedical Engineering, Engineering Faculty, Islamic Azad University, Mashhad, Iran
2- Department of Chemistry, Faculty of Basic Sciences, Ferdowsi University, Mashhad, Iran
3- Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- Department of Obstetrics and Gynecology, Mashhad University of Medical Sciences, Mashhad, Iran ,
5- Biochemistry of Nutrition Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6- Department of Microbiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
7- Cardiovascular Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
8- Department of Biomedical Engineering, Engineering Faculty, Islamic Azad University, Mashhad, Iran
Abstract: (2846 Views)
Background: Magnetized water has made many improvements in industry, agriculture and medicine. However its utilization in medicine still remains controversial.
Objective: In this study we aimed to investigate the effects of magnetized water on height of epithelial cells in pre-implantation stage endometrium and fallopian tube and number of corpus lutea in female mice.
Materials and Methods: Eighty female NRMI mice were recruited to this experimental study and randomly divided into two groups: the control group which drank normal water and the experimental (case) group which drank magnetized water for 2 weeks. Super-ovulation was induced in these mice and then they were mated with male mice as well. Samples of ovary, uterus and fallopian tube were obtained at the pre-implantation stage. Then, after preparation, the number of corpus lutea in each ovary was counted and the height of fallopian and endometrial epithelial cells was measured by light microscopy.
Results: Data analysis showed a significant increase in the mean number of corpus lutea and the height of epithelial cells in fallopian tube comparing the case with the control group (p=0.01, p=0.002 respectively) whereas uterus epithelial cells of the case group showed insignificant increase in height, in compare with the control group (p=0.052).
Conclusion: Our results suggest that magnetized water intake increases the number of corpus lutea and the height of fallopian tube epithelial cells. Further research is needed to determine whether this will increase in the success rate of fertility.
Objective: In this study we aimed to investigate the effects of magnetized water on height of epithelial cells in pre-implantation stage endometrium and fallopian tube and number of corpus lutea in female mice.
Materials and Methods: Eighty female NRMI mice were recruited to this experimental study and randomly divided into two groups: the control group which drank normal water and the experimental (case) group which drank magnetized water for 2 weeks. Super-ovulation was induced in these mice and then they were mated with male mice as well. Samples of ovary, uterus and fallopian tube were obtained at the pre-implantation stage. Then, after preparation, the number of corpus lutea in each ovary was counted and the height of fallopian and endometrial epithelial cells was measured by light microscopy.
Results: Data analysis showed a significant increase in the mean number of corpus lutea and the height of epithelial cells in fallopian tube comparing the case with the control group (p=0.01, p=0.002 respectively) whereas uterus epithelial cells of the case group showed insignificant increase in height, in compare with the control group (p=0.052).
Conclusion: Our results suggest that magnetized water intake increases the number of corpus lutea and the height of fallopian tube epithelial cells. Further research is needed to determine whether this will increase in the success rate of fertility.
Type of Study: Original Article |
References
1. Vahidi S, Ardalan A, Mohammad K. Prevalence of Primary Infertility in the Islamic Republic of Iran in 2004-2005. Int J Fertil Steril 2010; 4: 84.
2. Nygren KG, Sullivan E, Zegers-Hochschild F, Mansour R, Ishihara O, Adamson GD, et al.International Committee for Monitoring Assisted Reproductive Technology (ICMART) world report: assisted reproductive technology 2003. Fertil Steril 2011; 95: 2209-2222. [DOI:10.1016/j.fertnstert.2011.03.058]
3. Stern K. Assisted reproductive technology- What's new and what's important? Aust Fam Physician 2012; 41: 762-768.
4. Coey JMD, Cass S. Magnetic water treatment. Journal of Magnetism and Magnetic Materials 2000; 209: 71-74. [DOI:10.1016/S0304-8853(99)00648-4]
5. Sueda M, Katsuki A, Nonomura M, Kobayashi R, Tanimoto Y. Effects of High Magnetic Field on Water Surface Phenomena. The Journal of Physical Chemistry C 2007; 111: 14389-14393. [DOI:10.1021/jp072713a]
6. Cho YI, Lee S-H. Reduction in the surface tension of water due to physical water treatment for fouling control in heat exchangers. International Communications in Heat and Mass Transfer 2005; 32: 1-9. [DOI:10.1016/j.icheatmasstransfer.2004.03.019]
7. Nakagawa J, Hirota N, Kitazawa K, Shoda. Magnetic Field enhancement of water vaporization. J Appl Phys 1999; 86: 2923-2925. [DOI:10.1063/1.371144]
8. Iorio R, Delle Monache S, Bennato F, Di Bartolomeo C, Scrimaglio R, Cinque B, et al. Involvement of mitochondrial activity in mediating ELF-EMF stimulatory effect on human sperm motility. Bioelectromagnetics 2011; 32: 15-27. [DOI:10.1002/bem.20602]
9. Roychoudhury S, Jedlicka J, Parkanyi V, Rafay J, Ondruska L, Massanyi P, et al. Influence of a 50 hz extra low frequency electromagnetic field on spermatozoa motility and fertilization rates in rabbits. J Environ Sci Health A Tox Hazard Subst Environ Eng 2009; 44: 1041-1047. [DOI:10.1080/10934520902997029]
10. Wang D, Cheng X, Yan X. Effect of magnetized liquor on free radical metabolism in the heart of mice. Chinese Journal of Medical Physics 2002; 19: 243-244.
11. Raymond-Whish S, Mayer LP, O'Neal T, Martinez A, Sellers MA, Christian PJ, et al. Drinking water with uranium below the U.S. EPA water standard causes estrogen receptor-dependent responses in female mice. Environ Health Perspect 2007; 115: 1711-1716. [DOI:10.1289/ehp.9910]
12. O'Brien JE, Peterson TJ, Tong MH, Lee EJ, Pfaff LE, Hewitt SC, et al. Estrogen-induced proliferation of uterine epithelial cells is independent of estrogen receptor alpha binding to classical estrogen response elements. J Biol Chem 2006; 281: 26683-26692. [DOI:10.1074/jbc.M601522200]
13. Rajaei F, Borhani N, Sabbagh-Ziarani F, Mashayekhi F. Effects of extremely low-frequency electromagnetic field on fertility and heights of epithelial cells in pre-implantation stage endometrium and fallopian tube in mice. Zhong Xi Yi Jie He Xue Bao 2010; 8: 56-60. [DOI:10.3736/jcim20100111]
14. Gholizadeh M, Arabshahi H, Saeidi MR, Mahdavi B. The Effect of Magnetic Water on Growth and Quality Improvement of Poultry. Middle-East J Sci Res 2008; 3: 140-144.
15. Lin I, Yotvat J. Exposure of irrigation and drinking water to a magnetic field with controlled power and direction. J Magn Magn Mater 1990; 83: 525-526. [DOI:10.1016/0304-8853(90)90611-S]
16. Alimi F, Tlili M, Amor M, Gabrielli C, Maurin G. Influence of magnetic field on calcium carbonate precipitation. Desalination 2007; 206: 163-168. [DOI:10.1016/j.desal.2006.02.064]
17. Johnson KE, Sanders JJ, Gellin RG, Palesch YY. The effectiveness of a magnetized water oral irrigator (Hydro Floss) on plaque, calculus and gingival health. J Clin Periodontol 1998; 25: 316-321. [DOI:10.1111/j.1600-051X.1998.tb02447.x]
18. Lu Jf, Mao Yx, Yang Y, Wang Yc, Shi Yx, Zhou Qh. Influence of Magnetized Water on the Experimental Hyperlipemia and Atherosclerosis in Rabbits. Shanghai Lab Anim Sci 2000; 20: 45-51.
19. Hafizi-Lotfabadi L, Sazgarnia A, Mousavi-Far N, Karimi M, Ghorbani S, Kazemi M, et al. The Effect of Extremely Low Frequency Pulsed Electromagnetic Field on In-Vitro Fertilization Success Rate in NMRI Mice. Cell J 2014; 15: 310-315.
20. Agarwal A, Aponte-Mellado A, Premkumar BJ, Shaman A, Gupta S. The effects of oxidative stress on female reproduction: a review. Reprod Biol Endocrinol 2012; 10: 49. [DOI:10.1186/1477-7827-10-49]
21. Naher ZU, Ali M, Biswas SK, Mollah FH, Fatima P, Hossain MM, et al. Effect of oxidative stress in male infertility. Mymensingh Med J 2013; 22: 136-142.
22. Lee HJ, Kang MH. Effect of the magnetized water supplementation on blood glucose, lymphocyte DNA damage, antioxidant status, and lipid profiles in STZ-induced rats. Nutr Res Pract 2013; 7: 34-42. [DOI:10.4162/nrp.2013.7.1.34]
23. Ma YL, Ren H, Ren S, Zhen EK, Hao G, Zhao YW. A study of the effect of magnetized water on enzyme activities by potentiometric enzyme electrode method. J Tongji Med Univ 1992; 12: 193-196. [DOI:10.1007/BF02887847]
24. Bongso A, Ng SC, Fong CY, Ratnam S. Cocultures: a new lead in embryo quality improvement for assisted reproduction. Fertil Steril 1991; 56: 179-191. [DOI:10.1016/S0015-0282(16)54468-9]
25. Wagh PV, Lippes J. Human oviductal fluid proteins. V. Identification of human oviductin-I as alpha-fetoprotein. Fertil Steril 1993; 59: 148-56. [DOI:10.1016/S0015-0282(16)55631-3]
26. Shah D, Nagarajan N. Luteal insufficiency in first trimester. Indian J Endocrinol Metab 2013; 17: 44- 49. [DOI:10.4103/2230-8210.107834]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
