Volume 15, Issue 3 (5-2017)                   IJRM 2017, 15(3): 161-168 | Back to browse issues page

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Sabour M, Khoradmehr A, Kalantar S M, Danafar A H, Omidi M, Halvaei I, et al . Administration of high dose of methamphetamine has detrimental effects on sperm parameters and DNA integrity in mice. IJRM 2017; 15 (3) :161-168
URL: http://ijrm.ir/article-1-806-en.html
Administration of high dose of methamphetamine has detrimental effects on sperm parameters and DNA integrity in mice
Mojdeh Sabour1 , Arezoo Khoradmehr2 , Seyyed Mehdi Kalantar3 , Amir Hossein Danafar2 , Marjan Omidi2 , Iman Halvaei4 , Ali Nabi2 , Saeed Ghasemi- Esmailabad2 , Ali Reza Talebi * 5
1- Islamic Azad University of Ashkhezar, Yazd, Iran
2- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3- 1. Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 2. Department of General Surgery, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
4- Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
5- 1.Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.2.Department of Biology and Anatomy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. , prof_talebi@ssu.ac.ir
Abstract:   (4675 Views)
Background: Methamphetamine (MA) was shown to have harmful effects on malereproductive system.
Objective: To investigate probable effects of daily administration of MA on spermparameters and chromatin/DNA integrity in mouse.
Material and Methods: Thirty-five NMRI male mice were divided into five groupsincluding low, medium, and high dosage groups which were injectedintraperitoneally with 4, 8 and 15 mg/kg/day for 35 days, respectively. Normalsaline was injected in sham group and no medications were used in control group.Then, the mice were killed and caudal epididymis of each animal was cut and placedin Ham’s F10 medium for sperm retrieval. To evaluate sperm chromatinabnormalities, the aniline blue, toluidine blue and chromomycine A3 were used. Forsperm DNA integrity and apoptosis, the acridine orange, sperm chromatindispersion, and TUNEL assay were applied. For sperm morphology, Papanicolaoustaining was done
Results: Normal morphology and progressive motility of spermatozoa decreased inmedium and high dosage groups in comparison with the control group (p=0.035).There was a significant increase in rate of aniline blue, toluidine blue, andchromomycine A3 positive spermatozoa in high dosage group. In a similar manner,there was an increase in rates of acridine orange, TUNEL and sperm chromatindispersion positive sperm cells in high dosage group with respect to others.
Conclusion: MA abuse in a dose-dependent manner could have detrimental effectson male reproductive indices including sperm parameters and spermchromatin/DNA integrity in mice
Keywords: Methamphetamine, Apoptosis, Sperm, Sperm chromatin.
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Type of Study: Original Article |
References
1. Reuter PH. Can production and trafficking of illicit drugs be reduced or merely shifted? World Bank Policy Research Working Paper Series 2008; 1-35.
2. Schifano F, Corkery J, Cuffolo G. Smokable (ice, crystal meth) and non smokable amphetamine-type stimulants: clinical pharmacological and epidemiological issues, with special reference to the UK. Ann Ist Super Sanità 2007;43: 110-115.
3. Brick J, Erickson CK. Drugs, the brain, and behavior: The pharmacology of drug use disorders. 2nd Ed. Routledge; 2013.
4. Zhang X-D, Kelly-Hanku A, Chai J-J, Luo J, Temmerman M, Luchters S. Sexual and reproductive health risks amongst female adolescents who use amphetamine-type stimulants and sell sex: a qualitative inquiry in Yunnan, China. Harm Reduct J 2015; 34: 1-12. [DOI:10.1186/s12954-015-0065-y]
5. Kelly BC, LeClair A, Parsons JT. Methamphetamine use in club subcultures. Subst Use Misuse 2013; 48: 1541-1552. [DOI:10.3109/10826084.2013.808217]
6. Pal R, Megharaj M, Kirkbride KP, Naidu R. Illicit drugs and the environment-a review. Sci Total Environ 2013; 463: 1079-1092. [DOI:10.1016/j.scitotenv.2012.05.086]
7. Degenhardt L, Hall W. Extent of illicit drug use and dependence, and their contribution to the global burden of disease. Lancet 2012; 379: 55-70. [DOI:10.1016/S0140-6736(11)61138-0]
8. German CL, Fleckenstein AE, Hanson GR. Bath salts and synthetic cathinones: an emerging designer drug phenomenon. Life Sci 2014; 97: 2-8. [DOI:10.1016/j.lfs.2013.07.023]
9. Spauwen, M.H.F. The underlying biological mechanisms behind Helen Fisher's' Four primary temperament dimensions in the initial phase of mate choice' [Msc thesis]. Utrecht University, 2013.
10. Urióstegui-Acosta M, Hernández-Ochoa I, Sánchez-Gutiérrez M, Pi-a-Guzmán B, Rafael-Vázquez L, Solís-Heredia M, et al. Methamidophos alters sperm function and DNA at different stages of spermatogenesis in mice. Toxicol Appl Pharmacol 2014; 279: 391-400. [DOI:10.1016/j.taap.2014.06.017]
11. Singh P, Gupta R, Patidar D, Singh RK. Male infertility: causes and contributors. Int J Pharm Sci Res 2014; 5: 2095.
12. Wang L, Qu G, Dong X, Huang K, Kumar M, Ji L, et al. Long-term effects of methamphetamine exposure in adolescent mice on the future ovarian reserve in adulthood. Toxicol Lett 2016; 242: 1-8. [DOI:10.1016/j.toxlet.2015.11.029]
13. Fronczak CM, Kim ED, Barqawi AB. The insults of illicit drug use on male fertility. J Androl 2012; 33: 515-528. [DOI:10.2164/jandrol.110.011874]
14. Safarinejad MR, Asgari SA, Farshi A, Ghaedi G, Kolahi AA, Iravani S, et al. The effects of opiate consumption on serum reproductive hormone levels, sperm parameters, seminal plasma antioxidant capacity and sperm DNA integrity. Reprod Toxicol 2013; 36: 18-23. [DOI:10.1016/j.reprotox.2012.11.010]
15. Lin JF, Lin YH, Liao PC, Lin YC, Tsai TF, Chou KY, et al. Induction of testicular damage by daily methamphetamine administration in rats. Chin J Physiol 2014; 57: 19-30. [DOI:10.4077/CJP.2014.BAB155]
16. Nudmamud-Thanoi S, Sueudom W, Tangsrisakda N, Thanoi S. Changes of sperm quality and hormone receptors in the rat testis after exposure to methamphetamine. Drug Chem Toxicol 2016: 39: 432-438. [DOI:10.3109/01480545.2016.1141421]
17. Zuloaga DG, Johnson LA, Agam M, Raber J. Sex differences in activation of the hypothalamic-pituitary-adrenal axis by methamphetamine. J Neurochem 2014; 129: 495-508. [DOI:10.1111/jnc.12651]
18. Talebi A, Vahidi S, Aflatoonian A, Ghasemi N, Ghasemzadeh J, Firoozabadi R, et al. Cytochemical evaluation of sperm chromatin and DNA integrity in couples with unexplained recurrent spontaneous abortions. Andrologia 2012; 44: 462-470. [DOI:10.1111/j.1439-0272.2011.01206.x]
19. Carrell DT, Emery BR, Hammoud S. Altered protamine expression and diminished spermatogenesis: what is the link? Hum Reprod Update 2007; 13: 313-327. [DOI:10.1093/humupd/dml057]
20. Roychoudhury S, Sharma R, Sikka S, Agarwal A. Diagnostic application of total antioxidant capacity in seminal plasma to assess oxidative stress in male factor infertility. J Assist Reprod Genet 2016; 33: 627-635. [DOI:10.1007/s10815-016-0677-5]
21. Gharagozloo P, Gutiérrez-Adán A, Champroux A, Noblanc A, Kocer A, Calle A, et al. A novel antioxidant formulation designed to treat male infertility associated with oxidative stress: promising preclinical evidence from animal models. Hum Reprod 2016; 31: 252-262. [DOI:10.1093/humrep/dev302]
22. Vessey W, McDonald C, Virmani A, Almeida P, Jayasena C, Ramsay J. Levels of reactive oxygen species (ROS) in the seminal plasma predicts the effectiveness of L-carnitine to improve sperm function in men with infertility. Endocrine Abstracts 2016; 44: 232 [DOI:10.1530/endoabs.44.P232]
23. Green A, De Souza R, Williams J, Murray T, Cross A. The neurotoxic effects of methamphetamine on 5-hydroxytryptamine and dopamine in brain: evidence for the protective effect of chlormethiazole. Neuropharmacol 1992; 31: 315-321. [DOI:10.1016/0028-3908(92)90062-T]
24. Petropoulos S, Matthews SG, Szyf M. Adult glucocorticoid exposure leads to transcriptional and DNA methylation changes in nuclear steroid receptors in the hippocampus and kidney of mouse male offspring. Biol Reprod 2014; 90: 43. [DOI:10.1095/biolreprod.113.115899]
25. Talebi AR, Sarcheshmeh AA, Khalili MA, Tabibnejad N. Effects of ethanol consumption on chromatin condensation and DNA integrity of epididymal spermatozoa in rat. Alcohol 2011; 45: 403-409. [DOI:10.1016/j.alcohol.2010.10.005]
26. Roodbari F, Abedi N, Talebi AR. Early and late effects of Ibuprofen on mouse sperm parameters, chromatin condensation, and DNA integrity in mice. Iran J Reprod Med 2015; 13: 703.
27. WHO. WHO laboratory manual for the examination and processing of human semen. 5th Ed. Cambridge University Press; 2010.
28. Köhn F, Mack S, Schill W, Zaneveld L. Detection of human sperm acrosome reaction: comparison between methods using double staining, Pisum sativum agglutinin, concanavalin A and transmission electron microscopy. Hum Reprod 1997; 12: 714-721. [DOI:10.1093/humrep/12.4.714]
29. Auger J, Mesbah M, Huber C, Dadoune J. Aniline blue staining as a marker of sperm chromatin defects associated with different semen characteristics discriminates between proven fertile and suspected infertile men. Int J Androl 1990; 13: 452-462. [DOI:10.1111/j.1365-2605.1990.tb01052.x]
30. Mangoli E, Talebi AR, Anvari M, Pourentezari M. Effects of experimentally-induced diabetes on sperm parameters and chromatin quality in mice. Iran J Reprod Med 2013; 11: 53-60.
31. Rahimipour M, Talebi AR, Anvari M, Sarcheshmeh AA, Omidi M. Saccharin consumption increases sperm DNA fragmentation and apoptosis in mice. Iran J Reprod Med 2014; 12: 307-312.
32. Khoradmehr A, Danafar A, Halvaei I, Golzadeh J, Hosseini M, Mirjalili T, et al. Effect of prenatal methamphetamine administration during gestational days on mice. Iran J Reprod Med 2015; 13: 41-48.
33. Nabi A, Khalili M, Halvaei I, Roodbari F. Prolonged incubation of processed human spermatozoa will increase DNA fragmentation. Andrologia 2014; 46: 374-379. [DOI:10.1111/and.12088]
34. Alavi SH, Taghavi MM, Moallem SA. Evaluation of effects of methamphetamine repeated dosing on proliferation and apoptosis of rat germ cells. Syst Biol Reprod Med 2008; 54: 85-91. [DOI:10.1080/19396360801952078]
35. Nudmamud‐Thanoi S, Thanoi S. Methamphetamine induces abnormal sperm morphology, low sperm concentration and apoptosis in the testis of male rats. Andrologia 2011; 43: 278-282. [DOI:10.1111/j.1439-0272.2010.01071.x]
36. Yamamoto Y, Yamamoto K, Hayase T. Effect of methamphetamine on male mice fertility. J Obstet Gynaecol Res 1999; 25: 353-358. [DOI:10.1111/j.1447-0756.1999.tb01176.x]
37. Lin JF, Lin YH, Liao PC, Lin YC, Tsai TF, Chou KY, et al. Induction of testicular damage by daily methamphetamine administration in rats. Chin J Physiol 2014; 57: 19-30. [DOI:10.4077/CJP.2014.BAB155]
38. Barroso G, Morshedi M, Oehninger S. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum Reprod 2000; 15: 1338-1344. [DOI:10.1093/humrep/15.6.1338]
39. Frohmader KS, Bateman KL, Lehman MN, Coolen LM. Effects of methamphetamine on sexual performance and compulsive sex behavior in male rats. Psychopharmacol 2010; 212: 93-104. [DOI:10.1007/s00213-010-1930-8]
40. Lewis C, Dluzen DE. Testosterone enhances dopamine depletion by methamphetamine in male, but not female, mice. Neurosci Lett 2008; 448: 130-133. [DOI:10.1016/j.neulet.2008.10.011]
41. Kazerooni T, Asadi N, Jadid L, Kazerooni M, Ghanadi A, Ghaffarpasand F, et al. Evaluation of sperm's chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion. J Assist Reprod Genet 2009; 26: 591-596. [DOI:10.1007/s10815-009-9361-3]
42. Erenpreiss J, Jepson K, Giwercman A, Tsarev I, Erenpreisa J, Spano M. Toluidine blue cytometry test for sperm DNA conformation: comparison with the flow cytometric sperm chromatin structure and TUNEL assays. Hum Reprod 2004; 19: 2277-2282. [DOI:10.1093/humrep/deh417]
43. Barenys M, Macia N, Camps L, de Lapuente J, Gomez-Catalan J, Gonzalez-Linares J, et al. Chronic exposure to MDMA (ecstasy) increases DNA damage in sperm and alters testes histopathology in male rats. Toxicol Lett 2009; 191: 40-46. [DOI:10.1016/j.toxlet.2009.08.002]
44. Sakkas D, Mariethoz E, Manicardi G, Bizzaro D, Bianchi PG, Bianchi U. Origin of DNA damage in ejaculated human spermatozoa. Rev Reprod 1999; 4: 31-37. [DOI:10.1530/ror.0.0040031]
45. Said TM, Agarwal A, Sharma RK, Thomas AJ, Sikka SC. Impact of sperm morphology on DNA damage caused by oxidative stress induced by β-nicotinamide adenine dinucleotide phosphate. Fertil Steril 2005; 83: 95-103 [DOI:10.1016/j.fertnstert.2004.06.056]
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