en بیان mir-302 در جنین های انسانی حاصل از تزریق درون سیتوپلاسمی اسپرم به تخمک های بالغ شده در آزمایشگاه Original Article Original Article <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&nbsp;مقدمه:</span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">در یک سلول تخم در ابتدا رونوشت&shy;های مادری که مربوط به بلوغ تخمک هست باید پاک شود تا ژنوم جنینی فرصت فعال شدن را پیدا کنند. بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">mir-302</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در طول مراحل ابتدایی تکوین جنین، پاک شدن رونوشت&shy;های مادری را تنظیم می&shy;کند. </span></span><strong><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">در این مطالعه میزان بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">mir-302</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و اثر تکنیک بلوغ آزمایشگاهی (</span></span><span dir="LTR"><span style="font-size:10.0pt;">in vitro maturation</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) بر بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">mir-302</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در جنین</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های انسانی حاصل از &nbsp;&nbsp;تخمک</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های بالغ شده در آزمایشگاه بررسی شد.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">موارد و روش&shy;ها: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مطالعه حاضر بر اساس بلوغ تخمک در دو شرایط </span></span><span dir="LTR"><span style="font-size:10.0pt;">in vivo</span></span><span style="font-size:10.0pt;"> (</span><span dir="LTR"><span style="font-size:10.0pt;">MII</span></span><span style="font-size:10.0pt;">) </span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">و </span></span><span dir="LTR"><span style="font-size:10.0pt;">in vitro</span></span> <span dir="LTR"><span style="font-size:10.0pt;">(GV-MII)</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در دو گروه مورد بررسی قرار گرفت. تکوین و نمره کیفیت جنین تا روز سوم مورد ارزیابی قرار گرفت. در هر دو گروه جنین&shy;ها با مورفولوژی طبیعی وارد مطالعه مولکولی شده و با انجام </span></span><span dir="LTR"><span style="font-size:10.0pt;">PGD</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> (</span></span><span dir="LTR"><span style="font-size:10.0pt;">Preimplantation genetic diagnosis</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)، میزان بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">mir-302</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با استفاده از تکنیک </span></span><span dir="LTR"><span style="font-size:10.0pt;">qRT-PCR</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> مورد ارزیابی قرار گرفت. سپس جنین تحت فرایند انجماد قرار گرفت. </span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج نشان داد بیان نسبی </span></span><span dir="LTR"><span style="font-size:10.0pt;">mir-302</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> مابین دو گروه اختلاف معنی&shy;دار داشت (05/0></span></span><span dir="LTR"><span style="font-size:10.0pt;">p </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">). نمره کیفیت جنین مابین دو گروه اختلاف معنی</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">دار داشت (01/0></span></span><span dir="LTR"><span style="font-size:10.0pt;">p </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">).</span></span><strong><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه&shy; گیری:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">این مطالعه روشن کرد فرایند </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVM</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">اثری منفی بر روی سطح نسبی بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">mir-302</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">(علی</span></span><span style="font-size:11.0pt;">&shy;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">رغم مورفولوژی نرمال جنین</span></span><span style="font-size:11.0pt;">&shy;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ها) در جنین</span></span><span style="font-size:11.0pt;">&shy;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های پیش از لانه</span></span><span style="font-size:11.0pt;">&shy;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">گزینی دارد. بنابراین، با توجه به نقش</span></span><span style="font-size:11.0pt;">&shy;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های حیاتی </span></span><span dir="LTR"><span style="font-size:10.0pt;">mir-302</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، کاهش بیان این </span></span><span dir="LTR"><span style="font-size:10.0pt;">microRNA</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> می&shy;تواند با کاهش پتانسیل لازم برای تکوین ابتدایی جنین همراه باشد.</span></span> <strong>Background: </strong>The expression of miR-302 over the period of early embryogenesis could possibly regulate the maternal transcript clearance. Zygotic transcription activation is mostly related to maternal messages degradation.<br> <strong>Objective:</strong> In this study, the effects of in-vitro maturation technique (IVM) on the expression of miR-302 in human embryo produced from immature and mature human oocytes (matured in vitro and in vivo, before sperm exposure) obtained from females under gonadotrophin therapy were evaluated for assisted reproduction.<br> <strong>Materials and Methods:</strong> Immature oocytes were cultured in vitro. The injection of oocytes-producing polar bodies was given using fresh sperm. Then, the embryo quality score was assessed in the IVM group compared with the control group. In both the groups, embryos with normal morphology were included in the molecular study. Only one blastomere was removed from three-day embryos and then the embryos were frozen. The expression of miR-302 in embryos was measured through quantitative realtime polymerase chain reaction.<br> <strong>Results:</strong> Our data showed a significant reduction of miR-302 expression in the IVM group vs. the control group (p = 0.02). The embryo quality score showed a significant difference between the two groups (p = 0.01).<br> <strong>Conclusion:</strong> The present study demonstrated that the IVM process had a negative effect on the expression level of miR-302 in human pre-implantation embryos. Considering the major role of expression miR-302, a decrease in miR-302 expression<br> could be related to a&nbsp;reduced potential&nbsp; in the early embryonic development. mir-302, تکوین و نمو جنین, تحریک کنترل شده تخمدان, بلوغ آزمایشگاهی تخمک, تزریق داخل سیتوپلاسمی اسپرم. miR-302, Embryonic development, Ovarian stimulation, In-vitro maturation, Intracytoplasmic sperm injection. 405 412 http://ijrm.ir/browse.php?a_code=A-10-31-102&slc_lang=en&sid=1 Parvin Dorfeshan 0000-0001-9508-5737 No Department of Social Medicine, Faculty of Medicine, Jundishapure University of Medical Sciences, Ahvaz, Iran. Marefat Ghaffari Novin mghaffarin@yahoo.com 0000-0003-4455-5211 Yes Infertility and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Mohammad Salehi 0000-0002-3768-4325 No Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Fatane Farifteh 0000-0002-4421-8533 No Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran