en ارزیابی سطح بیان FZD3 و miR-378 در سلولهای کومولوس زنان مبتلا به سندرم تخمدان پلیکیستیک: یک مطالعه مورد-شاهدی Reproductive Genetics Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">سندرم تخمدان پلی&shy;کیستیک یک اختلال شایع غدد درون&shy;ریز است که زنان در سنین باروری را تحت تأثیر قرار می&shy;دهد. مطالعات اخیر نشان می&shy;دهد که </span></span><i><span dir="LTR" style="font-size:10.0pt">FZD3</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">miR-378</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با تأثیر بر بلوغ تخمک، نقش مهمی در </span></span><span dir="LTR" style="font-size:10.0pt">PCOS</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> دارند.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">با توجه به اهمیت </span></span><i><span dir="LTR" style="font-size:10.0pt">FZD3</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">miR-378</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در تخمک&shy;گذاری، مطالعه حاضر با هدف تعیین سطح بیان ژن </span></span><i><span dir="LTR" style="font-size:10.0pt">FZD3</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">miR-378</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در سلول&shy;های کومولوس تخمک&shy;های </span></span><span dir="LTR" style="font-size:10.0pt">GV</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">MII</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در زنان مبتلا به </span></span><span dir="LTR" style="font-size:10.0pt">PCOS</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> انجام شد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial",sans-serif"></span></span></b></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش&shy; ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نمونه‌های این مطالعه مورد-شاهدی شامل 25 زن مبتلا به سندرم تخمدان پلی&shy;کیستیک که در مرکز تحقیقاتی و بالینی ناباروری یزد تحت درمان قرار گرفتند، به‌طور تصادفی انتخاب شدند. تشخیص </span></span><span dir="LTR" style="font-size:10.0pt">PCOS</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بر اساس معیارهای تعریف شده در دستورالعمل روتردام انجام شد. واکنش زنجیره&shy;ای پلیمراز در زمان واقعی، سطح بیان </span></span><i><span dir="LTR" style="font-size:10.0pt">FZD3</span></i> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و </span></span><span dir="LTR" style="font-size:10.0pt">miR-378</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">را تعیین کرد.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه نشان داد که بیان </span></span><i><span dir="LTR" style="font-size:10.0pt">FZD3</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">miR-378</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در سلول&shy;های کومولوس تخمک&shy;های نابالغ در مقایسه با تخمک&shy;های بالغ افزایش یافته است </span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">(</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">0001/0</span></span> <span lang="FA" style="font-size:10.0pt"><span b="" mitra="" style="font-family:">> </span></span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""></span></span></b></span></span></span></span></span><br> <b><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه &shy;گیری:</span></span></b><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> سطوح بالای </span></span><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">FZD3</span></span></i> <span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">miR-378</span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در سلول&shy;های کومولوس تخمک&shy;های نابالغ می&shy;تواند بلوغ آنها را مهار کند. </span></span><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">FZD3</span></span></i><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، جزئی از مسیر سیگنالینگ </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">WNT</span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، در تخمک&shy;های نابالغ بیش از حد بیان می&shy;شود و ممکن است بر روند بلوغ تأثیر منفی بگذارد. علاوه بر این، </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">miR-378</span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با هدف قرار دادن و سرکوب ژن‌های ضروری، رشد تخمک را مهار می‌کند. در حال حاضر، جنبه&shy;های مختلف عملکرد </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">microRNA</span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> ناشناخته باقی مانده است. </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">miR-378</span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> ممکن است نقش تنظیمی خود را با هدف قرار دادن مستقیم ژن </span></span><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">FZD3</span></span></i> <span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">یا با هدف قرار دادن سایر ژن&shy;ها و واسطه&shy;هایی که با </span></span><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">FZD3</span></span></i> <span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">یا پروتئینی که آن را کدگذاری می&shy;کند، اعمال کند. این مطالعه ممکن است پایه&shy;ای برای بررسی بیشتر این فرضیه در تحقیقات آتی فراهم کند</span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Polycystic ovarian syndrome (PCOS) is a common endocrine disorder that affects women of reproductive age. Recent studies suggest that frizzled class receptor 3 (<i>FZD3</i>) and miR-378 play significant roles in PCOS by affecting oocyte maturation.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Considering the importance of <i>FZD3</i> and miR-378 in ovulation, the present study aimed to determine the expression levels of <i>FZD3</i> and <i>miR-378</i> genes in cumulus cells of germinal vesicles and metaphase II oocytes in women with PCOS.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The samples for this case-control study included, randomly selected, 25 women with PCOS who were treated at the </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Research and Clinical Center for Infertility, Yazd,</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Iran. The diagnosis of PCOS was made based on the criteria defined in the Rotterdam guidelines. </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Quantitative real-time polymerase chain reaction </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">determined the expression level of <i>FZD3</i> and miR-378.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">This study showed increased expression of <i>FZD3</i> and miR-378 in cumulus cells of immature oocytes compared to mature oocytes (p < 0.0001).</span></span></span></span></span><br> <b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">High levels of <i>FZD3</i> and miR-378 in cumulus cells of immature oocytes can inhibit their maturation. <i>FZD3</i>, a component of the WNT signaling pathway, is overexpressed in immature oocytes and may negatively affect the maturation process. Additionally, miR-378 inhibits oocyte development by targeting and repressing essential genes. Currently, various aspects of microRNA function remain unknown. MiR-378 may exert its regulatory role by directly targeting the <i>FZD3</i> gene or by targeting other genes and mediators that interact with <i>FZD3</i> or the protein it encodes. This study may provide a foundation for further investigation of this hypothesis in future research.</span></span></span></div> تنظیم بیان ژن, سندرم تخمدان پلی کیستیک, سلول های کومولوس,مسیر سیگنالیگ Wnt. Gene expression regulation, Polycystic ovary syndrome, Cumulus cells, Wnt signaling pathway. 265 274 http://ijrm.ir/browse.php?a_code=A-10-2930-1&slc_lang=en&sid=1 Amir Mohmmad Bagheri amirmo.bsh@gmail.com 0009-0006-8297-4766 No Department of Medical Genetics, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Kimia Monshizadeh kimia.monshizadeh@yahoo.com 0000-0003-2181-4890 No Department of Medical Genetics, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Fatemeh Anbari Anbari.fatemeh@gmail.comyahoo.com 0000-0001-6967-2305 No Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Nasrin Ghasemi nghasemi479@gmail.com 0000-0002-1103-6004 No Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Mohammadreza Dehghani mrdehghani@ssu.ac.ir 0000-0001-5134-9438 Yes 3. Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.