Volume 16, Issue 3 (March 2018)                   IJRM 2018, 16(3): 149-158 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ghowsi M, Khazali H, Sisakhtnezhad S. The effect of resveratrol on oxidative stress in the liver and serum of a rat model of polycystic ovary syndrome: An experimental study. IJRM 2018; 16 (3) :149-158
URL: http://ijrm.ir/article-1-1025-en.html
The effect of resveratrol on oxidative stress in the liver and serum of a rat model of polycystic ovary syndrome: An experimental study
Mahnaz Ghowsi1 , Homayoun Khazali * 2, Sajjad Sisakhtnezhad3
1- Department of Animal Physiology, Faculty of Biological Sciences and Technology, Shahid Beheshti University, Tehran, Iran
2- Department of Animal Physiology, Faculty of Biological Sciences and Technology, Shahid Beheshti University, Tehran, Iran. , khazhom@gmail.com
3- Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran
Abstract:   (3663 Views)
Background: Studies of oxidative status in polycystic ovarian syndrome (PCOS) patients are limited with inconsistent results. The effects of resveratrol as a natural antioxidant on oxidative status in PCOS aren’t clear.
Objective: This study evaluated effects of resveratrol on oxidative stress in the liver and serum of the PCOS rats.
Materials and Methods: Fifteen female Wistar rats (3 wk old) were divided into 3 groups (n=5/each e): Control group, PCO-Control group, and PCO-Resveratrol group. For induction of polycystic ovary phenotype, testosterone enanthate 10 mg/kg was injected for 35 days subcutaneously. Then, resveratrol 10 mg/kg was injected intraperitoneally for 28 days to rats of the PCO-Resveratrol group. Ovarian sections were stained with hematoxylin/eosin. The serum glucose and insulin and the levels of malondialdehyde (MDA) and total antioxidant capacity (TAC) in serum and liver were measured.
Results: Control animals showed normal ovarian morphology and PCO-Control animals exhibited cystic follicles. There were no significant differences in liver TAC between groups. The serum MDA (p=0.034), and homeostatic model assessment insulin resistance (HOMA-IR) (p=0.014) levels in PCO-Control rats were higher than the controls. The liver MDA in PCO-Control rats was more than that of controls (p=0.001). The HOMA-IR (p=0.008) and serum MDA (p=0.006) levels in PCO-Control rats were more than those of PCO-Resveratrol rats (p=0.008). In PCO-Resveratrol group, serum TAC was higher than that of PCO-Control group (p=0.022) and liver MDA was more than controls (p=0.01).
Conclusion: Results indicated that the induction of PCOS in rats increased lipid peroxidation and insulin resistance and resveratrol improved these complications.
Keywords: Oxidative stress, Polycystic ovary syndrome, Resveratrol, Liver.
Full-Text [PDF 744 kb]   (1269 Downloads) |   |   Full-Text (HTML)  (1002 Views)  
Type of Study: Original Article |
References
1. Rojas J, Chávez M, Olivar L, Rojas M, Morillo J, Mejías J, et al. Polycystic ovary syndrome, insulin resistance, and obesity: navigating the pathophysiologic labyrinth. Int J Reprod Med 2014; 2014: 1-17 [DOI:10.1155/2014/719050]
2. Sabuncu T, Vural H, Harma M, Harma M. Oxidative stress in polycystic ovary syndrome and its contribution to the risk of cardiovascular disease. Clin Biochem 2001; 34: 407-413. [DOI:10.1016/S0009-9120(01)00245-4]
3. Murri M, Luque-Ramírez M, Insenser M, Ojeda-Ojeda M, Escobar-Morreale HF. Circulating markers of oxidative stress and polycystic ovary syndrome (PCOS): a systematic review and meta-analysis. Hum Reprod Update 2013; 19: 268-288. [DOI:10.1093/humupd/dms059]
4. Torun AN, Vural M, Cece H, Camuzcuoglu H, Toy H, Aksoy N. Paraoxonase-1 is not affected in polycystic ovary syndrome without metabolic syndrome and insulin resistance, but oxidative stress is altered. Gynecol Endocrinol 2011; 27: 988-992. [DOI:10.3109/09513590.2011.569798]
5. González F, Rote NS, Minium J, Kirwan JP. Reactive oxygen species-induced oxidative stress in the development of insulin resistance and hyperandrogenism in polycystic ovary syndrome. J Clin Endocrinol Metab 2006; 91: 336-340. [DOI:10.1210/jc.2005-1696]
6. Rains JL, Jain SK. Oxidative stress, insulin signaling, and diabetes. Free Radic Biol Med 2011; 50: 567-575. [DOI:10.1016/j.freeradbiomed.2010.12.006]
7. Baltaci SB, Mogulkoc R, Baltaci AK. Resveratrol and exercise. Biomed Rep 2016; 5: 525-530. [DOI:10.3892/br.2016.777]
8. Su HC, Hung LM, Chen JK. Resveratrol, a red wine antioxidant, possesses an insulin-like effect in streptozotocin-induced diabetic rats. Am J Physiol Endocrinol Metab 2006; 290: E1339-E1346. [DOI:10.1152/ajpendo.00487.2005]
9. Tung BT, Rodríguez-Bies E, Ballesteros-Simarro M, Motilva V, Navas P, López-Lluch G. Modulation of endogenous antioxidant activity by resveratrol and exercise in mouse liver is age dependent. J Gerontol A: Biol Sci Med Sci 2014; 69: 398-409. [DOI:10.1093/gerona/glt102]
10. Beloosesky R, Gold R, Almog B, Sasson R, Dantes A, Land-Bracha A, et al. Induction of polycystic ovary by testosterone in immature female rats: modulation of apoptosis and attenuation of glucose/insulin ratio. Int J Mol Med 2004; 14: 207-216. [DOI:10.3892/ijmm.14.2.207]
11. Ergenoglu M, Yildirim N, Yildirim AG, Yeniel O, Erbas O, Yavasoglu A, et al. Effects of resveratrol on ovarian morphology, plasma anti-mullerian hormone, IGF-1 levels, and oxidative stress parameters in a rat model of polycystic ovary syndrome. Reprod Sci 2015; 22: 942-947. [DOI:10.1177/1933719115570900]
12. Asefi M, Vaisi‐Raygani A, Bahrehmand F, Kiani A, Rahimi Z, Nomani H, et al. Paraoxonase 1 (PON1) 55 polymorphism, lipid profiles and psoriasis. Br J Dermatol 2012; 167: 1279-1286. [DOI:10.1111/j.1365-2133.2012.11170.x]
13. Aref ABM, Ahmed OM, Ali LA, Semmler M. Maternal rat diabetes mellitus deleteriously affects insulin sensitivity and Beta-cell function in the offspring. J Diabet Res 2013; 2013: 1-10. [DOI:10.1155/2013/429154]
14. Giallauria F, Palomba S, Vigorito C, Tafuri MG, Colao A, Lombardi G, et al. Androgens in polycystic ovary syndrome: the role of exercise and diet. Semin Reprod Med 2009; 27: 306-315. [DOI:10.1055/s-0029-1225258]
15. Zordoky BN, Robertson IM, Dyck JR. Preclinical and clinical evidence for the role of resveratrol in the treatment of cardiovascular diseases. Biochim Biophys Acta 2015; 1852: 1155-1177. [DOI:10.1016/j.bbadis.2014.10.016]
16. Soufi FG, Mohammad-Nejad D, Ahmadieh H. Resveratrol improves diabetic retinopathy possibly through oxidative stress–nuclear factor κB–apoptosis pathway. Pharmacol Rep 2012; 64: 1505-1514. [DOI:10.1016/S1734-1140(12)70948-9]
17. Gregg D, Rauscher FM, Goldschmidt-Clermont PJ. Rac regulates cardiovascular superoxide through diverse molecular interactions: more than a binary GTP switch. Am J Physiol Cell Physiol 2003; 285: C723-C734. [DOI:10.1152/ajpcell.00230.2003]
18. Yeon Lee J, Baw C-K, Gupta S, Aziz N, Agarwal A. Role of oxidative stress in polycystic ovary syndrome. Curr Women's Health Rev 2010; 6: 96-107. [DOI:10.2174/157340410791321336]
19. Fenkci V, Fenkci S, Yilmazer M, Serteser M. Decreased total antioxidant status and increased oxidative stress in women with polycystic ovary syndrome may contribute to the risk of cardiovascular disease. Fertil Steril 2003; 80: 123-127. [DOI:10.1016/S0015-0282(03)00571-5]
20. Desai V, Prasad NR, Manohar SM, Sachan A, Narasimha SR, Bitla AR. Oxidative stress in non-obese women with polycystic ovarian syndrome. J Clin Diagn Res 2014; 8: CC01-CC03.
21. Takeda E, Arai H, Yamamoto H, Okumura H, Taketani Y. Control of oxidative stress and metabolic homeostasis by the suppression of postprandial hyperglycemia. J Med Invest 2005; 52 (Suppl.): 259-265. [DOI:10.2152/jmi.52.259]
22. Rubiolo JA, Mithieux G, Vega FV. Resveratrol protects primary rat hepatocytes against oxidative stress damage: Activation of the Nrf2 transcription factor and augmented activities of antioxidant enzymes. Eur J pharmacol 2008; 591: 66-72. [DOI:10.1016/j.ejphar.2008.06.067]
23. Sharma S, Anjaneyulu M, Kulkarni SK, Chopra K. Resveratrol, a polyphenolic phytoalexin, attenuates diabetic nephropathy in rats. Pharmacology 2006; 76: 69-75. [DOI:10.1159/000089720]
24. Kumar A, Kaundal RK, Iyer S, Sharma SS. Effects of resveratrol on nerve functions, oxidative stress and DNA fragmentation in experimental diabetic neuropathy. Life Sci 2007; 80: 1236-1244. [DOI:10.1016/j.lfs.2006.12.036]
25. Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, et al. The role of oxidative stress and antioxidants in liver diseases. Int J Mol Sci 2015; 16: 26087-26124. [DOI:10.3390/ijms161125942]
26. Marier JF, Vachon P, Gritsas A, Zhang J, Moreau JP, Ducharme MP. Metabolism and disposition of resveratrol in rats: extent of absorption, glucuronidation, and enterohepatic recirculation evidenced by a linked-rat model. J Pharmacol Exp Ther 2002; 302: 369-373. [DOI:10.1124/jpet.102.033340]
27. Klaassen CD, Reisman SA. Nrf2 the rescue: effects of the antioxidative/electrophilic response on the liver. Toxicol Appl Pharmacol 2010; 244: 57-65. [DOI:10.1016/j.taap.2010.01.013]
28. Sadi G, Bozan D, Yildiz HB. Redox regulation of antioxidant enzymes: post-translational modulation of catalase and glutathione peroxidase activity by resveratrol in diabetic rat liver. Mol Cell Biochem 2014; 393: 111-122. [DOI:10.1007/s11010-014-2051-1]
29. Misra A, Singhal N, Khurana L. Obesity, the metabolic syndrome, and type 2 diabetes in developing countries: role of dietary fats and oils. J Am Coll Nutr 2010; 29: 289S-301S. [DOI:10.1080/07315724.2010.10719844]
30. Bernardis LL, Patterson BD. Correlation between'Lee index'and carcass fat content in weanling and adult female rats with hypothalamic lesions. J Endocrinol 1968; 40: 527-528. [DOI:10.1677/joe.0.0400527]
31. Liu J, Zhang D. The role of oxidative stress in the pathogenesis of polycystic ovary syndrome. Sichuan Da Xue Xue Bao Yi Xue Ban 2012; 43: 187-190. (in Chinese)
32. Franks S. Polycystic ovary syndrome. N Engl J Med 1995; 333: 853-861. [DOI:10.1056/NEJM199509283331307]
33. Franks S. Polycystic ovary syndrome: a changing perspective. Clin Endocrinol 1989; 31: 87-120. [DOI:10.1111/j.1365-2265.1989.tb00457.x]
34. Le Lay S, Simard G, Martinez MC, Andriantsitohaina R. Oxidative stress and metabolic pathologies: from an adipocentric point of view. Oxid Med Cell Longev 2014; 2014: 908539. [DOI:10.1155/2014/908539]
35. Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, et al. Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest 2004; 114: 1752-1761. [DOI:10.1172/JCI21625]
36. Boden G, She P, Mozzoli M, Cheung P, Gumireddy K, Reddy P, et al. Free fatty acids produce insulin resistance and activate the proinflammatory nuclear factor-κB pathway in rat liver. Diabetes 2005; 54: 3458-3465. [DOI:10.2337/diabetes.54.12.3458]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb