Volume 16, Issue 6 (Jun 2018)
IJRM 2018, 16(6): 405-412 |
Back to browse issues page
Mona Ghasemi1 
, Abbas Farshad * 2, Hadi Hajarian3 , Omid Banafshi4 , Vahideh Asadollahi4 , Fardin Fathi5
, Abbas Farshad * 2, Hadi Hajarian3 , Omid Banafshi4 , Vahideh Asadollahi4 , Fardin Fathi5
1- Department of Animal Sciences, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran
2- Department of Animal Sciences, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran. , AFarshad1957@gmail.com
3- Department of Animal Sciences, Razi University, Kermanshah, Iran
4- Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
5- Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
2- Department of Animal Sciences, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran. , AFarshad1957@gmail.com
3- Department of Animal Sciences, Razi University, Kermanshah, Iran
4- Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
5- Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Abstract: (2798 Views)
Background: Sericin, because of its ability to remove free radicals and its antioxidant properties, has been used to successfully cryopreserve various mammalian cell types. However, the effects of sericin on cryopreservation of mouse sperm has not been reported.
Objective: The current study intended to determine the protective role of different concentrations of sericin (0, 0.25, 0.5, and 0.75%) on mouse spermatozoa during cryopreservation, in addition to its effect on in vitro fertilization and subsequent embryo development.
Materials and Methods: Mouse sperm from epididymides were frozen in cryoprotective agent with 18% raffinose, 3% skim milk, and different concentrations of sericin (0, 0.25, 0.5, 0.75%). Thawed sperm were used for in vitro fertilization. The obtained embryos were cultured in Ksom medium for 6 days. The post-thawed motility, viability, fertilizing ability, and subsequent development to the 2-cell embryo and blastocyst stages were evaluated. Results: Our findings show that frozen-thawed sperm cells with 5% sericin indicate significantly (p≤0.0001) percentages of survivability and motility, the best fertilizing ability, as well as 2-cell embryo and blastocyst development compared to the other treated groups. There was no significant difference in survivability (p=0.8781), fertilizing ability (p=0.2458) and development of 2-cell (p=0.5136) and blastocysts embryos (p=0.0896) between 0.75% sericin and control groups.
Conclusion: Supplementation by 0.5% sericin in cryoprotective agent improved frozen-thawed mouse epididymal sperm cell quality and resulted in increased embryo development.
Objective: The current study intended to determine the protective role of different concentrations of sericin (0, 0.25, 0.5, and 0.75%) on mouse spermatozoa during cryopreservation, in addition to its effect on in vitro fertilization and subsequent embryo development.
Materials and Methods: Mouse sperm from epididymides were frozen in cryoprotective agent with 18% raffinose, 3% skim milk, and different concentrations of sericin (0, 0.25, 0.5, 0.75%). Thawed sperm were used for in vitro fertilization. The obtained embryos were cultured in Ksom medium for 6 days. The post-thawed motility, viability, fertilizing ability, and subsequent development to the 2-cell embryo and blastocyst stages were evaluated. Results: Our findings show that frozen-thawed sperm cells with 5% sericin indicate significantly (p≤0.0001) percentages of survivability and motility, the best fertilizing ability, as well as 2-cell embryo and blastocyst development compared to the other treated groups. There was no significant difference in survivability (p=0.8781), fertilizing ability (p=0.2458) and development of 2-cell (p=0.5136) and blastocysts embryos (p=0.0896) between 0.75% sericin and control groups.
Conclusion: Supplementation by 0.5% sericin in cryoprotective agent improved frozen-thawed mouse epididymal sperm cell quality and resulted in increased embryo development.
Type of Study: Original Article |
References
1. Moore AI, Squires EL, Graham JK. Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival. Cryobiology 2005; 51: 241-249. [DOI:10.1016/j.cryobiol.2005.07.004]
2. Watson PF. The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci 2000; 60-61: 481-492. [DOI:10.1016/S0378-4320(00)00099-3]
3. Matsuoka T, Imai H, Kohno H, Fukui Y. Effects of bovine serum albumin and trehalose in semen diluents for improvement of frozen-thawed ram spermatozoa. J Reprod Dev 2006; 52: 675-683. [DOI:10.1262/jrd.18033]
4. .Songsasen N, Betteridge KJ, Leibo SP. Birth of live mice resulting from oocytes fertilized in vitro with cryopreserved spermatozoa. Biol Reprod 1997; 56: 143-152 [DOI:10.1095/biolreprod56.1.143]
5. .Nakagata N, Takeshima T. High fertilizing ability of mouse spermatozoa diluted slowly after cryopreservation. Theriogenology 1992; 37: 1283-1291. [DOI:10.1016/0093-691X(92)90183-R]
6. .Kato N, Sato S, Yamanaka A, Yamada H, Fuwa N, Nomura M. Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity. Biosci Biotechnol Biochem 1998; 62: 145-147. [DOI:10.1271/bbb.62.145]
7. Takahashi M, Tsujimoto K, Yamada H, Takagi H, Nakamori S. The silk protein, sericin, protects against cell death caused by acute serum deprivation in insect cell culture. Biotechnol Lett 2003; 25: 1805-1809. [DOI:10.1023/A:1026284620236]
8. Tsujimoto K, Takagi H, Takahashi M, Yamada H, Nakamori S. Cryoprotective effect of the serine-rich repetitive sequence in silk protein sericin. J Biochem 2001; 129: 979-986. [DOI:10.1093/oxfordjournals.jbchem.a002946]
9. Sasaki M, Kato Y, Yamada H, Terada S. Development of a novel serum-free freezing medium for mammalian cells using the silk protein sericin. Biotechnol Appl Biochem 2005; 42: 183-188. [DOI:10.1042/BA20050019]
10. Kumar P, Kumar D, Sikka P, Singh P. Sericin supplementation improves semen freezability of buffalo bulls by minimizing oxidative stress during cryopreservation. Anim Reprod Sci 2015; 152: 26-31. [DOI:10.1016/j.anireprosci.2014.11.015]
11. Hosoe M, Yoshida N, Hashiyada Y, Teramoto H, Takahashi T, Niimura S. Sericin accelerates the production of hyaluronan and decreases the incidence of polyspermy fertilization in bovine oocytes during in vitro maturation. J Reprod Dev 2014; 60: 268-273. [DOI:10.1262/jrd.2013-110]
12. Isobe T, Ikebata Y, Onitsuka T, Do LT, Sato Y, Taniguchi M, et al. Cryopreservation for bovine embryos in serum-free freezing medium containing silk protein sericin. Cryobiology 2013; 67: 184-187. [DOI:10.1016/j.cryobiol.2013.06.010]
13. Dash R, Acharya C, Bindu PC, Kundu SC. Antioxidant potential of silk protein sericin against hydrogen peroxide-induced oxidative stress in skin fibroblasts. BMB Rep 2008; 41: 236-241. [DOI:10.5483/BMBRep.2008.41.3.236]
14. Isobe T, Ikebata Y, Onitsuka T, Wittayarat M, Sato Y, Taniguchi M, et al. Effect of sericin on preimplantation development of bovine embryos cultured individually. Theriogenology 2012; 78: 747-752. [DOI:10.1016/j.theriogenology.2012.03.021]
15. Do LT, Namula Z, Luu VV, Sato Y, Taniguchi M, Isobe T, et al. Effect of sericin supplementation during in vitro maturation on the maturation, fertilization and development of porcine oocytes. Reprod Domest Anim 2014; 49: e17-e20. [DOI:10.1111/rda.12274]
16. Yasmin C, Otoi T, Setiadi MA, Karja NW. Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin. Acta Vet Hung 2015; 63: 110-117. [DOI:10.1556/AVet.2015.009]
17. Hajarian H, Aghaz F, Shabankareh HK. Replacement of serum with sericin in in vitro maturation and culture media: effects on embryonic developmental competence of Sanjabi sheep embryo during breeding season. Theriogenology; 92: 144-148. [DOI:10.1016/j.theriogenology.2016.12.027]
18. Cao TT, Zhang YQ. The potential of silk sericin protein as a serum substitute or an additive in cell culture and cryopreservation. Amino Acids 2017; 49: 1029-1039. [DOI:10.1007/s00726-017-2396-3]
19. Nakagata N. Cryopreservation of mouse spermatozoa and in vitro fertilization. Methods Mol Biol 2011; 693: 57-73. [DOI:10.1007/978-1-60761-974-1_4]
20. Takeo T, Nakagata N. Reduced glutathione enhances fertility of frozen/thawed C57BL/6 mouse sperm after exposure to methyl-beta-cyclodextrin. Biol Reprod 2011; 85: 1066-1072. [DOI:10.1095/biolreprod.111.092536]
21. Aboagla EM, Terada T. Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol Reprod 2003; 69: 1245-1250 [DOI:10.1095/biolreprod.103.017889]
22. Jain YC, Anand SR. The lipids of buffalo spermatozoa and seminal plasma. J Reprod Fertil 1976; 47: 255-260. [DOI:10.1530/jrf.0.0470255]
23. Aitken RJ, Buckingham D, Harkiss D. Use of a xanthine oxidase free radical generating system to investigate the cytotoxic effects of reactive oxygen species on human spermatozoa. J Reprod Fertil 1993; 97: 441-450. [DOI:10.1530/jrf.0.0970441]
24. Alvarez JG, Storey BT. Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Res 1989; 23: 77-90. [DOI:10.1002/mrd.1120230108]
25. Terada S, Nishimura T, Sasaki M, Yamada H, Miki M. Sericin, a protein derived from silkworms, accelerates the proliferation of several mammalian cell lines including a hybridoma. Cytotechnology 2002; 40: 3-12. [DOI:10.1023/A:1023993400608]
26. Aghaz F, Hajarian H, Shabankareh HK, Abdolmohammadi A. Effect of sericin supplementation in maturation medium on cumulus cell expansion, oocyte nuclear maturation, and subsequent embryo development in Sanjabi ewes during the breeding season. Theriogenology 2015; 84: 1631-1635. [DOI:10.1016/j.theriogenology.2015.08.013]
27. Ohnishi K, Murakami M, Morikawa M, Yamaguchi A.Effect of the silk protein sericin on cryopreserved rat islets. J Hepatobiliary Pancreat Sci 2012; 19: 354-360. [DOI:10.1007/s00534-011-0415-4]
28. Miyamoto Y, Oishi K, Yukawa H, Noguchi H, Sasaki M, Iwata H, et al. Cryopreservation of human adipose tissue-derived stem/progenitor cells using the silk protein sericin. Cell Transplant 2012; 21: 617-622. [DOI:10.3727/096368911X605556]
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |