چهارشنبه 26 اردیبهشت 1403
[Archive]
دوره 6، شماره 5 - ( 4-1387 )
جلد 6 شماره 5 صفحات 180-175 |
برگشت به فهرست نسخه ها
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Hassani Bafrani H, Saito H. Expression of c-Jun in human granulosa cells from patients participating in in vitro fertilization programs. IJRM 2008; 6 (5) :175-180
URL: http://ijrm.ir/article-1-128-fa.html
URL: http://ijrm.ir/article-1-128-fa.html
Expression of c-Jun in human granulosa cells from patients participating in in vitro fertilization programs. International Journal of Reproductive BioMedicine. 1387; 6 (5) :175-180
چکیده: (1921 مشاهده)
Background: The mitogen-activated protein kinase (MAPK) pathway is one of the major signaling pathways that transmit intracellular signals initiated by extracellular stimuli to the nucleus. The stress-activated protein kinase (SAPK)/c-Jun NH2-terminal kinase is a subfamily of MAP kinases implicated in cytokine and stress responses.
Objective: In this study, we have examined total and phosphorylated c-Jun in the mural and cumulus granulosa cells, and investigated also whether c-Jun can be responsible for the difference in the expression of apoptosis between mural and cumulus regions.
Materials and Methods: A total of 14 consecutive couples participating in IVF program were investigated. Aspirated follicular fluid was transferred into tissue culture dishes and oocyte-cumulus cells complexes were isolated. The cells were centrifuge and fixed with Bouin’s solution and then were put on a glass slide. After fixation, the slides were stained by immunocytochemistry method. The incidence of apoptotic granulosa cells was examined by a fluorescence microscope.
Results: The incidence of apoptotic granulosa cells was 1.27 ± 0.12 in the mural region and 0.38 ± 0.07 in the cumulus regions. All mural and cumulus cells expressed total c-Jun in 7 patients while phosphorylated c-Jun was also expressed in all cells of the other 7 patients. There was no difference between apoptotic and nonapoptotic cells in the expression of total and phosphorylated c-Jun.
Conclusion: C-Jun may not be responsible for apoptotic effect on mural and cumulus cells.
Objective: In this study, we have examined total and phosphorylated c-Jun in the mural and cumulus granulosa cells, and investigated also whether c-Jun can be responsible for the difference in the expression of apoptosis between mural and cumulus regions.
Materials and Methods: A total of 14 consecutive couples participating in IVF program were investigated. Aspirated follicular fluid was transferred into tissue culture dishes and oocyte-cumulus cells complexes were isolated. The cells were centrifuge and fixed with Bouin’s solution and then were put on a glass slide. After fixation, the slides were stained by immunocytochemistry method. The incidence of apoptotic granulosa cells was examined by a fluorescence microscope.
Results: The incidence of apoptotic granulosa cells was 1.27 ± 0.12 in the mural region and 0.38 ± 0.07 in the cumulus regions. All mural and cumulus cells expressed total c-Jun in 7 patients while phosphorylated c-Jun was also expressed in all cells of the other 7 patients. There was no difference between apoptotic and nonapoptotic cells in the expression of total and phosphorylated c-Jun.
Conclusion: C-Jun may not be responsible for apoptotic effect on mural and cumulus cells.
نوع مطالعه: Original Article |
فهرست منابع
1. Kidd VJ. Proteolytic activities that mediate apoptosis. Annu Rev Physiol 1998; 60: 533-573. [DOI:10.1146/annurev.physiol.60.1.533]
2. Earnshaw WC, Martins LM, Kaufmann SH. Mammalian caspases: structure, activation, substrates, and functions during apoptosis. Annu Rev Biochem 1999; 68: 383-424. [DOI:10.1146/annurev.biochem.68.1.383]
3. Kerr JFR, Gobe, GC, Winterford CM, Harmon BV. Anatomical methods in cell death. In: Schwartz LM, Osborne B (eds). Methods in cell biology, New York: Academic Press; 1998; 1-27. [DOI:10.1016/S0091-679X(08)61921-4]
4. Ben-Sasson SA, Sherman Y, Gavrieli Y. Identification of dying cells-in situ staining. Anatomical methods in cell death. In: Schwartz LM, Osborne B (eds). Methods in cell Biology. New York: Academic press; 1995; 29-39. [DOI:10.1016/S0091-679X(08)61922-6]
5. Eastman A. Assays for DNA fragmantation, endonucleases and intracellular PH and Ca2+ associated with apoptosis. Anatomical methods in cell death. In: Schwartz LM, Osborne B (eds). Methods in Cell Biology. New York: Academic Press; 1995; 41-55. [DOI:10.1016/S0091-679X(08)61923-8]
6. Casas C, Ribera J, Esquedra JE. Antibodies against c-Jun N-terminal peptide cross-react with neo-epitopes emerging after caspase-mediated proteolysis during apoptosis. J Neurochem 2001; 77: 904-915. [DOI:10.1046/j.1471-4159.2001.00314.x]
7. Minden A, Karin M. Regulation and function of the JNK subgroup of MAP kinases. Biochim Biophys Acta 1997; 1333: 85-104. [DOI:10.1016/S0304-419X(97)00018-8]
8. IP YT, Davis RJ. Signal transduction by the c-jun N-terminal kinase (JNK)-from inflammation to development. Curr Opin Cell Biol 1998; 10: 205-219. [DOI:10.1016/S0955-0674(98)80143-9]
9. Schaeffer HJ, Weber MJ. Mitogen-activated protein kinases: specific messages from ubiquitous messengers. Mol Cell Biol 1999; 19: 2435-2444. [DOI:10.1128/MCB.19.4.2435]
10. Boulton TG, Nye SH, Robbins DJ, IP NY, Radziejewska E, Morgenbesser SD,et al. ERKs: a family of protein-serine/threnine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF. Cell 1991; 65: 663-675. [DOI:10.1016/0092-8674(91)90098-J]
11. Han J, Lee JD, Bibbs L, Ulevitch RJ. A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. Science 1994; 265: 808-811. [DOI:10.1126/science.7914033]
12. Cobb MH, Goldsmith EJ. How MAP kinases are regulated. L Biol Chem 1995; 270: 14843-14846. [DOI:10.1074/jbc.270.25.14843]
13. Kyriakis JM, Avruch J. Protein kinase cascades activated by stress and inflammatory cytokines. Bioessays 1996; 18: 567-577. [DOI:10.1002/bies.950180708]
14. Su B, Karin M. Mitogen-activated protein kinase cascades and regulation of gene expression. Curr Opin Immunol 1996; 8: 402-411. [DOI:10.1016/S0952-7915(96)80131-2]
15. Fanger GR, Gerwins P, Widmann C, Jarpe MB, Johnson GL. MEKKS, GCKs, MLKs, PAKs, TAKs, and tpls: upstream regulators of the c-jun amino-terminal kinases? Curr Opin Genet Dev 1997; 7: 67-74. [DOI:10.1016/S0959-437X(97)80111-6]
16. Kim JW, Chang TS, Lee JE, Huh SH, Yeon SW, Yang WS, et al. Negative regulation of the SAPK/JNK signaling pathway by presenilin 1. The Journal of Cell Biology 2001; 153: 457-464. [DOI:10.1083/jcb.153.3.457]
17. Bagowski PC, Xiong W, Ferrel EJ. C-jun N-terminal kinase activation in xenopus laevis eggs and embryos. The J Biol Chem 2001; 276: 1459-1465. [DOI:10.1074/jbc.M008050200]
18. Estus S, Zaks WJ, Freeman RS, Gruda M, Bravo R, Johnson EM. Altered gene expression in neurons during programmed cell death: identification of c-Jun as necessary for neuronal apoptosis. J Cell Biol 1994; 127: 1717-1727. [DOI:10.1083/jcb.127.6.1717]
19. Ham J, Babij C, Whitfield J, Pfarr CM, Lallemand D, Yaniv, et al. A c-Jun dominant negative mutant protects sympathetic neurons against programmed cell death. Neuron 1995; 14: 927-939. [DOI:10.1016/0896-6273(95)90331-3]
20. Wang CY, Mayo MW, Baldwin JR. TNF- and cancer therapy-induced apoptosis: potentiation by inhibition of NF-KappaB. Science 1996; 274: 784-787. [DOI:10.1126/science.274.5288.784]
21. Le-Niculescu H, Bonfoco E, Kasuya Y, Claret FX, Green DR, Karin M. Withdrawal of survival factors results in activation of the JNK pathway in neuronal cells leading to fas ligand induction and cell death. Mol Cell Biol 1999; 19: 751-763. [DOI:10.1128/MCB.19.1.751]
22. Kolbus A, Herr I, Martin S, Debatin KM, Wagner EF, Angel P. c-Jun-dependent CD95-L Expression is a rate-limiting step in the induction of apoptosis by alkylating agents. Mol Cell Biol 2000; 20: 575-582. [DOI:10.1128/MCB.20.2.575-582.2000]
23. Leoncini L, Del Vecchio MT, Megha T, Barbini P, Galieni P, Pileri S. Correlation between apoptotic and proliferative indices in malignant non-Hodgkin's lymphoma. Am J Pathol 1993; 142: 755-763.
24. Wheeler Tm, Rogers E, Aihara M, Scardino PT, Thompson TC. Apoptotic index as a biomarker in prostatic intraepithelial neoplasm (PIN) and prostatic cancer. J Cell Biol 1994; 19: 202-207.
25. Nakahara K, Sakai N, Saito H, Naohiro T, Saito T, Hiroi M, et al. Incidence of apoptotic bodies in membrane granulosa of patients participating in an in vitro fertilization program. Fertil Steril 1997; 67: 302-308. [DOI:10.1016/S0015-0282(97)81915-2]
26. Amsterdam A, Koch Y, Leiberman Me, Lindner HR. Distribution of binding sites for human chorionic gonadotropin in the preovlatory follicle of the rat. J Cell Biol 1975; 67: 894-900. [DOI:10.1083/jcb.67.3.894]
27. Lawrence TS, Deckel N, Beers WH. Binding of human chorionic gonadotropin by rat cumuli oophori and granulosa cells: a comparative study. Endocrinology 1980; 106: 1114-1118. [DOI:10.1210/endo-106-4-1114]
28. Zhou J, Refuerzo J, Bondy C. Granulosa cell DNA synthesis is strictly correlated with the presence of insulinlike growth factor I and absence of c-fos/c-jun expression. Mol Endocrinol 1995; 9: 924-931. [DOI:10.1210/me.9.7.924]
29. Oktay KH, Oktay MH. Immunohistochemical analysis of tyrosine phosphorylation and AP-1 transcription factors c-Jun, Jun D, and Fos family during early ovarian follicle development in the mouse. Appl Immunohistochem Mol Morpho 2004; 12:364-369. [DOI:10.1097/00129039-200412000-00014]
30. Peluso JJ, Luciano AM, Pappalardo A, White BA. Cellular and molecular mechanisms that mediate insulin-dependent rat granulosa cell mitosis. Biol Reproduction 1995; 52: 124-130. [DOI:10.1095/biolreprod52.1.124]
31. Son DS, Arai KY, Roby KF, Terranova PF. Tumor necrosis factor alpha (TNF) increases granulosa cell proliferation: dependence on c-Jun and TNF receptor type 1. Endocrinology 2004; 145: 1218-1226. [DOI:10.1210/en.2003-0860]
32. Quinn P, Warnes GM, Kerin JF, Kirby C. Culture factors affecting the success rate of in vitro fertilization and embryo transfer. Ann NY Acad Sci 1985; 442: 195-204. [DOI:10.1111/j.1749-6632.1985.tb37520.x]
33. Saporito SM, Thomas AB, Scott WR. MPTP activates c-Jun NH2-terminal kinase (JNK) and its upstream regulatory kinase MKK4 in nigrostriatal neurons in vivo. J Neurochem 2000; 75: 1200-1208. [DOI:10.1046/j.1471-4159.2000.0751200.x]
34. Schaeffer JH, Weber JM: Mitogen-Activated Protein Kinases. Specific messages from ubiquitous messengers. Mol Cell Biol 1999; 19: 2435-2444. [DOI:10.1128/MCB.19.4.2435]
| بازنشر اطلاعات | |
 |
این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است. |
