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Reproductive Biomedicine
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Volume 6, Issue 5 (7-2008)
IJRM 2008, 6(5): 175-180 |
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Hassani Bafrani H, Saito H. Expression of c-Jun in human granulosa cells from patients participating in in vitro fertilization programs. IJRM 2008; 6 (5) :175-180
URL: http://ijrm.ir/article-1-128-en.html
URL: http://ijrm.ir/article-1-128-en.html
1- Department of Anatomy and Embryology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran , hasanhasani@yahoo.com
2- Department of Obstetrics and Gynecology, School of Medicine, Yamagata University of Medical Sciences, Yamagata, Japan
2- Department of Obstetrics and Gynecology, School of Medicine, Yamagata University of Medical Sciences, Yamagata, Japan
Abstract: (2142 Views)
Background: The mitogen-activated protein kinase (MAPK) pathway is one of the major signaling pathways that transmit intracellular signals initiated by extracellular stimuli to the nucleus. The stress-activated protein kinase (SAPK)/c-Jun NH2-terminal kinase is a subfamily of MAP kinases implicated in cytokine and stress responses.
Objective: In this study, we have examined total and phosphorylated c-Jun in the mural and cumulus granulosa cells, and investigated also whether c-Jun can be responsible for the difference in the expression of apoptosis between mural and cumulus regions.
Materials and Methods: A total of 14 consecutive couples participating in IVF program were investigated. Aspirated follicular fluid was transferred into tissue culture dishes and oocyte-cumulus cells complexes were isolated. The cells were centrifuge and fixed with Bouin’s solution and then were put on a glass slide. After fixation, the slides were stained by immunocytochemistry method. The incidence of apoptotic granulosa cells was examined by a fluorescence microscope.
Results: The incidence of apoptotic granulosa cells was 1.27 ± 0.12 in the mural region and 0.38 ± 0.07 in the cumulus regions. All mural and cumulus cells expressed total c-Jun in 7 patients while phosphorylated c-Jun was also expressed in all cells of the other 7 patients. There was no difference between apoptotic and nonapoptotic cells in the expression of total and phosphorylated c-Jun.
Conclusion: C-Jun may not be responsible for apoptotic effect on mural and cumulus cells.
Objective: In this study, we have examined total and phosphorylated c-Jun in the mural and cumulus granulosa cells, and investigated also whether c-Jun can be responsible for the difference in the expression of apoptosis between mural and cumulus regions.
Materials and Methods: A total of 14 consecutive couples participating in IVF program were investigated. Aspirated follicular fluid was transferred into tissue culture dishes and oocyte-cumulus cells complexes were isolated. The cells were centrifuge and fixed with Bouin’s solution and then were put on a glass slide. After fixation, the slides were stained by immunocytochemistry method. The incidence of apoptotic granulosa cells was examined by a fluorescence microscope.
Results: The incidence of apoptotic granulosa cells was 1.27 ± 0.12 in the mural region and 0.38 ± 0.07 in the cumulus regions. All mural and cumulus cells expressed total c-Jun in 7 patients while phosphorylated c-Jun was also expressed in all cells of the other 7 patients. There was no difference between apoptotic and nonapoptotic cells in the expression of total and phosphorylated c-Jun.
Conclusion: C-Jun may not be responsible for apoptotic effect on mural and cumulus cells.
Type of Study: Original Article |
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