International Journal of
Reproductive Biomedicine
Mon, Dec 30, 2024
[Archive]
Volume 18, Issue 4 (April 2020)
IJRM 2020, 18(4): 295-306 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Sadeghzadeh F, Sadeghzadeh A, Changizi-Ashtiyani S, Bakhshi S, Jalali Mashayekhi F, Mashayekhi M, et al . The effect of hydro-alcoholic extract of Ceratonia Silique L. on spermatogenesis index in rats treated with cyclophosphamide: An experimental study. IJRM 2020; 18 (4) :295-306
URL: http://ijrm.ir/article-1-1351-en.html
URL: http://ijrm.ir/article-1-1351-en.html
Firouzeh Sadeghzadeh1 
, Azizeh Sadeghzadeh1 , Saeed Changizi-Ashtiyani * 2, Sepideh Bakhshi1 , Farideh Jalali Mashayekhi1 , Mehry Mashayekhi3 , Hossein Poorcheraghi4 , Ali Zarei5 , Mostafa Jafari6
, Azizeh Sadeghzadeh1 , Saeed Changizi-Ashtiyani * 2, Sepideh Bakhshi1 , Farideh Jalali Mashayekhi1 , Mehry Mashayekhi3 , Hossein Poorcheraghi4 , Ali Zarei5 , Mostafa Jafari6
1- Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Iran.
2- Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Iran. ,dr.ashtiyani@arakmu.ac.ir
3- Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
4- Student Research Committee, Arak University of Medical Sciences, Arak, Iran.
5- Department of Physiology, Shiraz University of Medical Sciences, Shiraz, Iran.
6- Department of Neurology, University of Minnesota, Minneapolis, USA.
2- Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Iran. ,
3- Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
4- Student Research Committee, Arak University of Medical Sciences, Arak, Iran.
5- Department of Physiology, Shiraz University of Medical Sciences, Shiraz, Iran.
6- Department of Neurology, University of Minnesota, Minneapolis, USA.
Abstract: (2856 Views)
Background: Ceratonia silique (Ceratonia) is a medicinal herb with antioxidant properties that reduces oxidative stress.
Objective: The aim of this study was to investigate the antioxidant effects of Ceratonia extract on improving the toxicity induced by cyclophosphamide (CP) on spermatogenesis.
Materials and Methods: 54 male Wistar rats (4 months old) weighing 200-250 gr were randomly divided into 6 groups (n = 9/each): “group 1 (control) underwent the normal diet and water; group 2 (sham) received 2 ml/day normal saline; group 3 (positive control) received 300 mg/kg/day Ceratonia extract; group 4 (Ceratonia + CP) received Ceratonia extract (300 mg/kg/day) + 5 mg/kg/day CP (Endoxan, baxter oncology gmbh, Germany) after 4 hr; group 5 (CP) received 5 mg/kg/day CP + normal saline 4 hr after it; and group 6 (CP + Ceratonia) received Ceratonia extract (300 mg/kg/day) 4 hr after 5 mg/kg/day CP.” 24 hr after the last gavage, heart blood sampling was performed to measure the levels of malondialdehyde (MDA), ferric reducing antioxidant power, testosterone, luteinizing hormone, and follicle-stimulating hormone. The left caudal epididymis was cut in the Ham’s F10 and the released spermatozoa were used to analyze sperm parameters. The histology of the right testes was studied using stereological techniques and the left testes were used to measure the level of tissue MDA and ferric reducing antioxidant power.
Results: A significant increase in the mean level of MDA (p = 0.013) was seen in the CP compared to the control group. Sperm motility (p = 0.001) and count (p = 0.002), serum and tissue total antioxidant (p ≤ 0.001) and serum testosterone levels (p = 0.019) decreased in the CP compared to the control group. Ceratonia extract could significantly prevent the adverse effects of CP on sperm motility (p < 0.001), the mean levels of tissue MDA (p = 0.018), serum total antioxidant (p = 0.045), and testosterone (p < 0.001).
Conclusion: The Ceratonia extract can modify the reproductive toxicity of CP in rat due to the presence of antioxidant compounds.
Objective: The aim of this study was to investigate the antioxidant effects of Ceratonia extract on improving the toxicity induced by cyclophosphamide (CP) on spermatogenesis.
Materials and Methods: 54 male Wistar rats (4 months old) weighing 200-250 gr were randomly divided into 6 groups (n = 9/each): “group 1 (control) underwent the normal diet and water; group 2 (sham) received 2 ml/day normal saline; group 3 (positive control) received 300 mg/kg/day Ceratonia extract; group 4 (Ceratonia + CP) received Ceratonia extract (300 mg/kg/day) + 5 mg/kg/day CP (Endoxan, baxter oncology gmbh, Germany) after 4 hr; group 5 (CP) received 5 mg/kg/day CP + normal saline 4 hr after it; and group 6 (CP + Ceratonia) received Ceratonia extract (300 mg/kg/day) 4 hr after 5 mg/kg/day CP.” 24 hr after the last gavage, heart blood sampling was performed to measure the levels of malondialdehyde (MDA), ferric reducing antioxidant power, testosterone, luteinizing hormone, and follicle-stimulating hormone. The left caudal epididymis was cut in the Ham’s F10 and the released spermatozoa were used to analyze sperm parameters. The histology of the right testes was studied using stereological techniques and the left testes were used to measure the level of tissue MDA and ferric reducing antioxidant power.
Results: A significant increase in the mean level of MDA (p = 0.013) was seen in the CP compared to the control group. Sperm motility (p = 0.001) and count (p = 0.002), serum and tissue total antioxidant (p ≤ 0.001) and serum testosterone levels (p = 0.019) decreased in the CP compared to the control group. Ceratonia extract could significantly prevent the adverse effects of CP on sperm motility (p < 0.001), the mean levels of tissue MDA (p = 0.018), serum total antioxidant (p = 0.045), and testosterone (p < 0.001).
Conclusion: The Ceratonia extract can modify the reproductive toxicity of CP in rat due to the presence of antioxidant compounds.
Type of Study: Original Article |
Subject:
Fertility & Infertility
References
1. Sadeghzadeh F, Mehranjani MS, Mahmoodi M. Vitamin C ameliorates the adverse effects of dexamethasone on sperm motility, testosterone level and spermatogenesis indexes in mice. Hum Exp Toxicol 2019; 38: 409-418. [DOI:10.1177/0960327118816137]
2. Bakhtiary Z, Shahrooz R, Ahmadi A, Malekinejad H, Mostafavi M. [Study of protective effect of crocin on testiculat histomorphometry and serological parameters in cyclophosphamid on treated adult mice]. J Urmia Univ Med Sci 2014; 25: 663-672. (in Persian)
3. Jalali AS, Hasanzadeh S, Malekinejad H. Crataegus monogyna aqueous extract ameliorates cyclophosphamide-induced toxicity in rat testis: stereological evidences. Acta Med Iran 2012; 50: 1-8.
4. Hamzeh M, Hosseinimehr SJ, Karimpour A, Mohammadi HR, Khalatbary AR, Talebpour Amiri FT. Cerium oxide nanoparticles protect cyclophosphamide-induced testicular toxicity in mice. Int J Prev Med 2019; 10: 5. [DOI:10.4103/ijpvm.IJPVM_184_18]
5. Ghobadi E, Moloudizargari M, Asghari MH, Abdollahi M. The mechanisms of cyclophosphamide-induced testicular toxicity and the protective agents. Exp Opin Drug Metab Toxicol 2017; 13: 525-536. [DOI:10.1080/17425255.2017.1277205]
6. Razak RNHA, Ismail F, Isa MLM, Wahab AYA, Muhammad H, Ramli R, et al. Ameliorative effects of aquilaria malaccensis leaves aqueous extract on reproductive toxicity induced by cyclophosphamide in male rats. Malays J Med Sci 2019; 26: 44-57.
7. Chabra A, Shokrzadeh M, Naghshvar F, Salehi F, Ahmadi A. Melatonin ameliorates oxidative stress and reproductive toxicity induced by cyclophosphamide in male mice. Hum Exp Toxicol 2014; 33: 185-195. [DOI:10.1177/0960327113489052]
8. Mehraban Z, Ghaffari Novin M, Golmohammadi MG, Sagha M, Pouriran K, Nazarian H. Protective effect of gallic acid on apoptosis of sperm and in vitro fertilization in adult male mice treated with cyclophosphamide. J Cell Biochem 2019; 120: 17250-17257. [DOI:10.1002/jcb.28987]
9. Najafi H, Changizi-Ashtiyani S, Najafi M. Antioxidant activity of omega-3 derivatives and their delivery via nanocages and nanocones: DFT and experimental in vivo investigation. J Mol Model 2017; 23: 326-337. [DOI:10.1007/s00894-017-3504-8]
10. Rtibi K, Selmi S, Grami D, Amri M, Eto B, El-Benna J, et al. Chemical constituents and pharmacological actions of carob pods and leaves (Ceratonia siliqua L.) on the gastrointestinal tract: A review. Biomed Pharmacother 2017; 93: 522-528. [DOI:10.1016/j.biopha.2017.06.088]
11. Ata A, Yildiz-Gulay O, Güngör S, Balic A, Gulay MS. The effect of carob (Ceratonia siliqua) bean extract on male New Zealand White rabbit semen. World Rabbit Scie 2018; 26: 209-215. [DOI:10.4995/wrs.2018.10154]
12. Souli A, Sebai H, Chehimi L, Rtibi K, Tounsi H, Boubaker S, et al. Hepatoprotective effect of carob against acute ethanol-induced oxidative stress in rat. Toxicol Ind Health 2015; 31: 802-810. [DOI:10.1177/0748233713475506]
13. Vafaei A, Mohammadi S, Fazel A, Soukhtanloo M, Mohammadi pour A, Beheshti F. Effects of carob (ceratonia siliqua) on sperm quality, testicular structure, testosterone level and oxidative stress in busulfan-induced infertile mice. Pharm Sci 2018; 24: 104-111. [DOI:10.15171/PS.2018.16]
14. Mokhtari M, Mohammadi B, Sharifi E, Shahamir-Tabaabaee M. [The effect of hydro - alcoholic seeds extract of Ceratonia siliqua L. on the kidney functional factors in diabetic male rats]. Yafte 2011; 13: 74-82. (in Persian)
15. Anan H, Wahba N, Abdollah M. Histologycal and immunologycal study of cyclophosphamide effect on adult rat testis. Int J Sci Rep 2017; 3: 39-48. [DOI:10.18203/issn.2454-2156.IntJSciRep20170356]
16. Ghosh D, Das UB, Ghosh S, Mallick M, Debnath J. Testicular gametogenic and steroidogenic activities in cyclophosphamide treated rat: A correlative study with testicular oxidative stress. Drug Chem Toxicol 2002; 25: 281-292. [DOI:10.1081/DCT-120005891]
17. Ramezani M, Changizi-Ashtiyani S, Sadeghzadeh F, Hosseini SS, Zarei A, Hosseini N. Effect of Hydroalcoholic Extract of Some Medicinal Plants on Obesity. Eur J Med Plants 2018; 24: 1-10. [DOI:10.9734/EJMP/2018/41111]
18. Momeni HR, Soleimani Mehranjani M, Abnosi MH, Mahmoodi M. Effects of vitamin E on sperm parameters and reproductive hormones in developing rats treated with para-nonylphenol. Int J Reprode Med 2009; 7: 111-116.
19. Malmir M, Soleimani Mehranjani M, Naderi Noreini S, Faraji T. Protective antioxidant effects of N‐acetylcysteine against impairment of spermatogenesis caused by paranonylphenol. Andrologia 2018; 50: e13114. [DOI:10.1111/and.13114]
20. Tarozzi N, Bizzaro D, Flamigni C, Borini A. Clinical relevance of sperm DNA damage in assisted reproduction. Reprod Biomed Online 2007; 14: 746-757. [DOI:10.1016/S1472-6483(10)60678-5]
21. Afkhami-Ardakani M, Hasanzadeh S, Shahrooz R, Delirezh N, Malekinejad H. Antioxidant effects of Spirulina platensis (Arthrospira platensis) on cyclophosphamide-induced testicular injury in rats. Vet Res Forum 2018; 9: 35-41.
22. Salimnejad R, Soleimani Rad J, Mohammad Nejad D, Roshangar L. Effect of ghrelin on total antioxidant capacity, lipid peroxidation, sperm parameters and fertility in mice against oxidative damage caused by cyclophosphamide. Andrologia 2018; 50: e12883. [DOI:10.1111/and.12883]
23. Mehraban Z, Ghaffari Novin M, Golmohammadi MG, Sagha M, Ziai SA, Abdollahifar MA, et al. Protective effect of gallic acid on testicular tissue, sperm parameters, and dna fragmentation against toxicity induced by cyclophosphamide in adult NMRI mice. Urol J 2019; 18: doi: 10.22037.
24. Soleimanzadeh A, Kian M, Moradi S, Mahmoudi S. Carob (Ceratonia siliqua L.) fruit hydro-alcoholic extract alleviates reproductive toxicity of lead in male mice: Evidence on sperm parameters, sex hormones, oxidative stress biomarkers and expression of Nrf2 and iNOS. Avicenna J Phytomed 2020; 10: 35-49.
25. Shalizar Jalali A, Hasanzadeh S, Malekinejad H. Achillea millefolium inflorescence aqueous extract ameliorates cyclophosphamide-induced toxicity in rat testis: stereological evidences. Chin J Nat Med 2012; 10: 247-254. [DOI:10.3724/SP.J.1009.2012.00247]
26. Torabi F, Malekzadeh Shafaroudi M, Rezaei N. Combined protective effect of zinc oxide nanoparticles and melatonin on cyclophosphamide-induced toxicity in testicular histology and sperm parameters in adult Wistar rats. Int J Reprod BioMed 2017; 15: 403-412. [DOI:10.29252/ijrm.15.7.403]
27. Nayak G, Honguntikar SD, Kalthur SG, D'Souza AS, Mutalik S, Setty MM, et al. Ethanolic extract of moringa oleifera Lam. leaves protect the pre-pubertal spermatogonial cells from cyclophosphamide-induced damage. J Ethnopharmacol 2016; 182: 101-109. [DOI:10.1016/j.jep.2016.02.003]
28. Elhalim SAA, Sharada HM, Abulyazid I, Aboulthana WM, Elhalim STA. Ameliorative effect of carob pods extract (ceratonia siliqua l.) against cyclophosphamide induced alterations in bone marrow and spleen of rats. J Appl Pharm Sci 2017; 7: 168-181.
29. Mahdiani E, Khadem Haghighian H, Javadi M, Karami AA, Kavianpour M. [Effect of Carob (Ceratonia siliqua L.) oral supplementation on changes of semen parameters, oxidative stress, inflammatory biomarkers and reproductive hormones in infertile men]. Sci J Kurdistan Univ Med Sci 2018; 23: 56-66. (in Persian)
30. Mokhtari M, Sharifi E, Azadian Sh. The effects of hydro alcoholic extract of Ceratonia siliqua L. seeds on pituitary--testis hormones and spermatogenesis in rat. Adv Environment Biol 2012; 23: 2778-2784.
31. Lev E, Amar Z. Ethnopharmacological survey of traditional drugs sold in the Kingdom of Jordan. J Ethnopharmacol 2002; 82: 131-145. [DOI:10.1016/S0378-8741(02)00182-4]
32. Liu M, Tan H, Zhang X, Liu Z, Cheng Y, Wang D, et al. Hematopoietic effects and mechanisms of Fufang e׳jiao jiang on radiotherapy and chemotherapy-induced myelosuppressed mice. J Ethnopharmacol 2014; 152: 575-584. [DOI:10.1016/j.jep.2014.02.012]
33. Tripathi DN, Jena GB. Astaxanthin inhibits cytotoxic and genotoxic effects of cyclophosphamide in mice germ cells. Toxicology 2008; 248: 96-103. [DOI:10.1016/j.tox.2008.03.015]
34. Mahmoudzadeh L, Najafi H, Ashtiyani SC, Yarijani ZM. Anti-inflammatory and protective effects of saffron extract in ischaemia/reperfusion-induced acute kidney injury. Nephrology 2017; 22: 748-754. [DOI:10.1111/nep.12849]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
