Volume 17, Issue 2 (February 2019 2019)                   IJRM 2019, 17(2): 99-106 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Salehi P, Shahrokhi S Z, Kamran T, Ajami A, Taghiyar S, Deemeh M R. Effect of antioxidant therapy on the sperm DNA integrity improvement; a longitudinal cohort study. IJRM 2019; 17 (2) :99-106
URL: http://ijrm.ir/article-1-1399-en.html
Effect of antioxidant therapy on the sperm DNA integrity improvement; a longitudinal cohort study
Peyman Salehi1 , Seyedeh Zahra Shahrokhi2 , Tayyebeh Kamran3 , Ali Ajami4 , Sana Taghiyar4 , Mohammad Reza Deemeh * 5
1- Infertility center, Shahid Beheshti Hospital, Isfahan, Iran
2- Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3- Department of Midwifery, Shahid Beheshti Hospital, Isfahan, Iran.
4- Andrology Section, Nobel Mega-lab, Isfahan, Iran
5- Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , m.deemeh@modares.ac.ir
Abstract:   (5103 Views)
Background: The effect of antioxidant therapy on sperm DNA fragmentation index (DFI) and achieving natural pregnancy were under debate. Very few studies have showed the rate of pregnancy rate after the antioxidant therapy due to ethical and technical limitations.
Objective: The aim of this cohort study was to determine the improvement rate of sperm DFI and natural pregnancy rate after the antioxidant therapy in infertile men.
Materials and Methods: 1645 infertile men were subjected for this study from May 2015 to December 2017. The Spermogram and sperm DFI were assessed using World Health Organization (WHO) 2010-based protocols and sperm chromatin structure assay (SCSA), respectively, in sperm samples before and after antioxidant therapy.
Results: The total sperm DFI improvement rate was 38.9% in the total population. Sperm DFI improvement had close correlation with total motility (r= 0.731, p= 0.001) and progressive motility improvement (r= 0.885, p= 0.001); 16.8% of individuals who completed antioxidant therapy for nine months achieved natural pregnancy.
Conclusion: The results of the current study suggested that SCSA along with spermogram might be a suitable option for the evaluation of fertility potential. In addition, antioxidant therapy may be useful for men with high levels of sperm DFI. However, the rate of pregnancy was still low and other treatment protocols such as assisted reproductive technology may be necessary.
Keywords: Antioxidant, Reactive oxygen species, DNA fragmentation, Male infertility
Full-Text [PDF 1205 kb]   (1232 Downloads) |   |   Full-Text (HTML)  (494 Views)  
Type of Study: Original Article |
References
1. [1] Menkveld R, Wong WY, Lombard CJ, Wetzels AM, Thomas CM, Merkus HM, et al. Semen parameters, including WHO and strict criteria morphology, in a fertile and subfertile population: an effort towards standardization of in-vivo thresholds. Hum Reprod 2001; 16: 1165–1171. [DOI:10.1093/humrep/16.6.1165]
2. [2] Palermo GD, Neri QV, Cozzubbo T, Rosenwaks Z. Perspectives on the assessment of human sperm chromatin integrity. Fertil Steril 2014; 102: 1508–1517. [DOI:10.1016/j.fertnstert.2014.10.008]
3. [3] Aitken RJ, De Iuliis GN. Value of DNA integrity assays for fertility evaluation. Soc Reprod Fertil 2007; 65 (Suppl.): 81–92.
4. [4] Simon L, Murphy K, Shamsi MB, Liu L, Emery B, Aston KI, et al. Paternal influence of sperm DNA integrity on early embryonic development. Hum Reprod 2014; 29: 2402–2412. [DOI:10.1093/humrep/deu228]
5. [5] Oleszczuk K, Giwercman A, Bungum M. Sperm chromatin structure assay in prediction of in vitro fertilization outcome. Andrology 2016; 4: 290–296. [DOI:10.1111/andr.12153]
6. [6] Bisht S, Dada R. Oxidative stress: Major executioner in disease pathology, role in sperm DNA damage and preventive strategies. Front Biosci (Schol Ed) 2017; 9: 420–447. [DOI:10.2741/s495]
7. [7] Lopes S, Jurisicova A, Sun JG, Casper RF. Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa. Hum Reprod 1998; 13: 896–900. [DOI:10.1093/humrep/13.4.896]
8. [8] Aitken RJ, De Iuliis GN, Finnie JM, Hedges A, McLachlan RI. Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria. Hum Reprod 2010; 25: 2415–2426. [DOI:10.1093/humrep/deq214]
9. [9] Tarin JJ, Brines J, Cano A. Antioxidants may protect against infertility. Hum Reprod 1998; 13: 1415–1416. [DOI:10.1093/humrep/13.6.1415]
10. [10] Sheweita SA, Tilmisany AM, Al-Sawaf H. Mechanisms of male infertility: role of antioxidants. Curr Drug Metab 2005;6: 495–501. [DOI:10.2174/138920005774330594]
11. [11] Imamovic Kumalic S, Pinter B. Review of clinical trials on effects of oral antioxidants on basic semen and other parameters in idiopathic oligoasthenoteratozoospermia. Biomed Res Int 2014; 2014: 426951–426961.
12. [12] Lombardo F, Sansone A, Romanelli F, Paoli D, Gandini L, Lenzi A. The role of antioxidant therapy in the treatment of male infertility: an overview. Asian J Androl 2011; 13: 690–697. [DOI:10.1038/aja.2010.183]
13. [13] World Health Organization. Examination and processing human semen. 5th Ed. New York, Cambridge University Press; 2010.
14. [14] Boe-Hansen GB, Fedder J, Ersboll AK, Christensen P. The sperm chromatin structure assay as a diagnostic tool in the human fertility clinic. Hum Reprod 2006; 21: 1576–1582. [DOI:10.1093/humrep/del019]
15. [15] Evenson DP, Larson KL, Jost LK. Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. J Androl 2002; 23: 25–43. [DOI:10.1002/j.1939-4640.2002.tb02599.x]
16. [16] Evgeni E, Charalabopoulos K, Asimakopoulos B. Human sperm DNA fragmentation and its correlation with conventional semen parameters. J Reprod Infertil 2014; 15: 2–14.
17. [17] Aitken RJ, Baker MA. Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development. Int J Dev Biol 2013; 57: 265–272. [DOI:10.1387/ijdb.130146ja]
18. [18] Aitken RJ. Reactive oxygen species as mediators of sperm capacitation and pathological damage. Mol Reprod Dev 2017; 84: 1039–1052. [DOI:10.1002/mrd.22871]
19. [19] Cuasnicu PS, Da Ros VG, Weigel Munoz M, Cohen DJ. Acrosome reaction as a preparation for gamete fusion. Adv Anat Embryol Cell Biol 2016; 220: 159–172. [DOI:10.1007/978-3-319-30567-7_9]
20. [20] Showell MG, Mackenzie-Proctor R, Brown J, Yazdani A, Stankiewicz MT, Hart RJ. Antioxidants for male subfertility. Cochrane Database Syst Rev 2014; 12: CD007411. [DOI:10.1002/14651858.CD007411.pub3]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb