یکشنبه 9 دی 1403
[Archive]
دوره 7، شماره 2 - ( 4-1388 )
جلد 7 شماره 2 صفحات 84-79 |
برگشت به فهرست نسخه ها
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Kilic S, Yuksel B, Yilmaz N, Ozdemir E, Ozturk U, Ceylaner S et al . Results of ICSI in severe oligozoospermic and azoospermic patients with AZF microdeletions. IJRM 2009; 7 (2) :79-84
URL: http://ijrm.ir/article-1-142-fa.html
URL: http://ijrm.ir/article-1-142-fa.html
Results of ICSI in severe oligozoospermic and azoospermic patients with AZF microdeletions. International Journal of Reproductive BioMedicine. 1388; 7 (2) :79-84
چکیده: (2443 مشاهده)
Background: The aim of this study was to determine the incidence of AZF (Azoospermia Factor) microdeletions of the Y chromosome in infertile Turkish male patients and intracytoplasmic sperm injection (ICSI) outcome of these patients.
Objective: This study was undertaken in order to evaluate the outcome of intracytoplasmic sperm injection (ICSI) in infertil man with AZF microdeletions Materials and Methods: We evaluated 348 azoospermic and oligozoospermic patients retrospectively. Fourty of these patients had various types of AZF microdeletions. These patients had non-obstructive severe oligoasthenospermia or azoospermia with normal karyotype. Azoospermic patients underwent testicular sperm extraction and aspiration (TESE, TESA). Then ICSI was performed to patients who had testicular sperm or ejeculat.
Results: Fourty patients with AZF microdeletion were evaluated in this study. No spermium could be found in 27 patients. Three of these patients had only AZFa microdeletion, three had AZFb microdeletion, three had AZF (b+c), six had AZF (a+b+c) and 12 patients had AZFc microdeletion. Only two of all patients achieved a pregnancy and both had only AZFc microdeletion.
Conclusion: AZFc microdeletions have a better prognosis for achieving spermium in ejaculate or TESE, TESA materials.
Objective: This study was undertaken in order to evaluate the outcome of intracytoplasmic sperm injection (ICSI) in infertil man with AZF microdeletions Materials and Methods: We evaluated 348 azoospermic and oligozoospermic patients retrospectively. Fourty of these patients had various types of AZF microdeletions. These patients had non-obstructive severe oligoasthenospermia or azoospermia with normal karyotype. Azoospermic patients underwent testicular sperm extraction and aspiration (TESE, TESA). Then ICSI was performed to patients who had testicular sperm or ejeculat.
Results: Fourty patients with AZF microdeletion were evaluated in this study. No spermium could be found in 27 patients. Three of these patients had only AZFa microdeletion, three had AZFb microdeletion, three had AZF (b+c), six had AZF (a+b+c) and 12 patients had AZFc microdeletion. Only two of all patients achieved a pregnancy and both had only AZFc microdeletion.
Conclusion: AZFc microdeletions have a better prognosis for achieving spermium in ejaculate or TESE, TESA materials.
نوع مطالعه: Original Article |
فهرست منابع
1. Tiepolo L, Zuffardi O. Localization of factors controlling spermatogenesis in the nonfluorescent portion of the human Y chromosome long arm. Hum Genet 1976; 34:119-1124. [DOI:10.1007/BF00278879]
2. Matsuda T, Horii Y, Ogura K, Nonomura M, Okada K, Yoshida O. Chromosomal survey of 1001 subfertile males: incidence and clinical features of males with chromosomal anomalies. Acta Urol Jpn 1992; 38: 803-809.
3. Ristanovic M, Bunjevacki V, Tulic C, Novakovic I, Nikolic A. Molecular analysis of Y chromosome microdeletions in idiopathic cases of male infertility in Serbia. Genetika 2007; 43: 850-854. [DOI:10.1134/S1022795407060154]
4. Choi JM, Chung P, Veeck L, Mielnik A, Palermo GD, Schlegel PN. AZF microdeletions of the Y chromosome and in vitro fertilization outcome. Fertil Steril 2004; 81: 337-341. [DOI:10.1016/j.fertnstert.2003.06.030]
5. Ferlin A, Arredi B, Speltra E, Cazzadore C, Selice R, Garolla A, et al. Molecular and clinical characterization of Y chromosome microdeletions in infertile men: a 10-year experience in Italy. Clin Endocrinol Metab 2007; 92: 762-770. [DOI:10.1210/jc.2006-1981]
6. Tsujimura A, Matsumiya K, Miyagawa Y, Tohda A, Miura H, Nishimura K, et al. Conventional multiple or microdissection testicular sperm extraction: a comparative study. Hum Reprod 2002; 17: 2924-2929. [DOI:10.1093/humrep/17.11.2924]
7. Schlegel PN. Testicular sperm extraction: Microdissection improves sperm yield with minimal tissue excision. Hum Reprod 1999;14: 131-135. [DOI:10.1093/humrep/14.1.131]
8. Simoni M, Bakker E, Krausz C. EAA/EMQN best practice guidelines for moleculer diognosis of y-chromosomal microdeletions. Int J Androl 2004,27: 240-249. [DOI:10.1111/j.1365-2605.2004.00495.x]
9. Hadj-Kacem L, Hadj-Kacem H, Ayadi H, Ammar-Keskes L, Chakroun-Fki N, Rebai T, et al. Screening of Y chromosome microdeletions in Tunisian infertile men. Arch Androl 2006; 52: 169-174. [DOI:10.1080/01485010500397964]
10. Krausz C, Bussani-Mastellone C, Granchi S, McElreavey K, Scarselli G, Forti G. Screening for microdeletions of Y chromosome genes in patients undergoing intracytoplasmic sperm injection. Hum Reprod 1999; 14: 1717-1721. [DOI:10.1093/humrep/14.7.1717]
11. Martínez MC, Bernabé MJ, Gómez E, Ballesteros A, Landeras J, Glover G, et al. Screening for AZF deletion in a large series of severely impaired spermatogenesis patients. J Androl 2000; 21: 651-655.
12. Sargin CF, Berker-Karaüzüm S, Manguoğlu E, Erdoğru T, Karaveli S, Gülkesen KH, et al. AZF microdeletions on the Y chromosome of infertile men from Turkey. Ann Genet 2004 ;47: 61-68. [DOI:10.1016/j.anngen.2003.09.002]
13. Kamp C, Hirschmann P, Voss H, Huellen K, Vogt PH. Two long homologous retroviral sequence blocks in proximal Yq11 cause AZFa microdeletions as a result of intrachromosomal recombination events. Hum Mol Genet 2000; 9: 2563-2572. [DOI:10.1093/hmg/9.17.2563]
14. Yogev L, Segal S, Zeharia E, Gamzu R, Maymon BB, Paz G, et al. Sex chromosome alignment at meiosis of azoospermic men with azoospermia factor microdeletion. J Androl 2004; 25: 110-116. [DOI:10.1002/j.1939-4640.2004.tb02765.x]
15. Luetjens CM, Gromoll J, Engelhardt M, Von Eckardstein S, Bergmann M, Nieschlag E, et al. Manifestation of Y-chromosomal deletions in the human testis: a morphometrical and immunohistochemical evaluation. Hum Reprod 2002; 17: 2258-2266. [DOI:10.1093/humrep/17.9.2258]
16. Plaseski T, Novevski P, Kocevska B, Dimitrovski C, Efremov GD, Plaseska-Karanfilska D. AZF deletions in infertile men from the Republic of Macedonia Prilozi 2006; 27: 5-16.
17. Luetjens CM, Gromoll J, Engelhardt M, Von Eckardstein S, Bergmann M, Nieschlag E, et al. Manifestation of Y-chromosomal deletions in the human testis: a morphometrical and immunohistochemical evaluation. Hum Reprod 2002; 17: 2258-2266. [DOI:10.1093/humrep/17.9.2258]
18. Oates RD, Silber S, Brown LG, Page DC. Clinical characterization of 42 oligospermic or azoospermic men with microdeletion of the AZFc region of the Y chromosome, and of 18 children conceived via ICSI. Hum Reprod 2002; 17: 2813-2824. [DOI:10.1093/humrep/17.11.2813]
19. Lynch M, Cram DS, Reilly A, O'Bryan MK, Baker HW, de Kretser DM, et al. The Y chromosome gr/gr subdeletion is associated with male infertility. Mol Hum Reprod 2005; 11: 507-512. [DOI:10.1093/molehr/gah191]
20. Amer M, Haggar SE, Moustafa T, Abd El-Naser T, Zohdy W. Testicular sperm extraction: impact of testicular histology on outcome, number of biopsies to be performed and optimal time for repetition. Hum Reprod 1999; 14: 3030-3034. [DOI:10.1093/humrep/14.12.3030]
| بازنشر اطلاعات | |
 |
این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است. |
