Volume 7, Issue 2 (7-2009)                   IJRM 2009, 7(2): 45-52 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Abedelahi A, Salehnia M, Abdolamir A, Hajizadeh E. Comparison of ultrastructure and morphology of mouse ovarian follicles after conventional and direct cover vitrification using different concentrations of ethylene glycol. IJRM 2009; 7 (2) :45-52
URL: http://ijrm.ir/article-1-147-en.html
Comparison of ultrastructure and morphology of mouse ovarian follicles after conventional and direct cover vitrification using different concentrations of ethylene glycol
Ali Abedelahi1 , Mojdeh Salehnia * 2, Allameh Abdolamir3 , Ebrahim Hajizadeh4
1- Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , mogdeh@dr.com
3- Department of Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4- Department of Biostatistics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (2675 Views)
Background: Many attempts have done to improve cryopreservation of mammalian ovaries using simple, economical and efficient technique “vitrification”.
Objective: The aim of the present study was to compare the mouse ovaries cryopreservation by direct cover vitrification (DCV) using different concentrations of ethylene glycol (EG) with conventional vitrification methods (CV).
Materials and Methods: Ninety NMRI mice were sacrificed by cervical dislocation; their ovaries were divided into three main experimental groups: control or non-vitrified group, CV group and DCV groups with 4, 6 and 8M EG as cryoprotectant. After vitrification-warming, the viability of mechanically isolated follicles and the morphology of ovarian follicles by light and electron microscopes were studied.
Results: The normality of primary and preantral follicles in non-vitrified and CV groups were higher than those achieved by DCV groups (p<0.001). The survival rates of isolated follicles in non-vitrified, CV and DCV groups with 4M, 6M and 8M ethylene glycol were 98.32, 96.26, 84.10, 85.46 and 84.56 %, respectively and in DCV groups it was lower than other groups (p<0.001). The ultrastructure of ovarian follicles was well preserved in CV technique. The follicles in DCV groups appeared to have vacuolated oocyte with nuclear shrinkage and irregular distribution of cytoplasmic organelles. Their mitochondria were located mainly in the sub cortical part of the oocyte and the granulosa cells demonstrated some signs of degeneration.
Conclusion: DCV of mouse ovarian tissue using only EG has induced some alteration on the fine structure of follicles. The integrity of mouse ovarian tissue was affected by DCV technique more than CV.
Keywords: Cryopreservation, Direct cover vitrification, Ethylene glycol, Preantral follicle, Ultrastructure.
Full-Text [PDF 348 kb]   (621 Downloads) |   |   Full-Text (HTML)  (490 Views)  
Type of Study: Original Article |
References
1. Bagchi A, Woods EJ, Critser JK. Cryopreservation and vitrification: recent advances in fertility preservation technologies. Expert Rev Med Devices 2008; 5: 359-370. [DOI:10.1586/17434440.5.3.359]
2. Courbiere B, Odagescu V, Baudot A, Massardier J, Mazoyer C, Salle B, et al. Cryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols. Fertil Steril 2006; 86: 1243-1251. [DOI:10.1016/j.fertnstert.2006.05.019]
3. Li YB, Zhou CQ, Yang GF, Wang Q, Dong Y. Modified vitrification method for cryopreservation of human ovarian tissues. Chin Med J 2007; 120: 110-114. [DOI:10.1097/00029330-200701020-00007]
4. Migishima F, Suzuki-Migishima R, Song SY, Kuramochi T, Azuma S, Nishijima M, et al. Successful cryopreservation of mouse ovaries by vitrification. Biol Reprod 2003; 68: 881-887. [DOI:10.1095/biolreprod.102.007948]
5. Salehnia M, Moghadam EA, Velojerdi MR. Ultrastructural of follicles after vitrification of mouse ovarian tissue. Fertil Steril 2002; 78: 644-645. [DOI:10.1016/S0015-0282(02)03287-9]
6. Choi J, Lee JK, Lee E, Yoon BK, Bae D, Choi D. Cryopreservation of the mouse ovary inhibits the onset of primordial follicle development. Cryobiology 2007; 54: 55-62. [DOI:10.1016/j.cryobiol.2006.11.003]
7. Choi WJ, Yeo HJ, Shin JK, Lee SA, Lee JH, Paik WY. Effect of vitrification method on survivability, follicular growth and ovulation of preantral follicles in mice. J Obstet Gynaecol Res 2007; 33: 128-133. [DOI:10.1111/j.1447-0756.2007.00498.x]
8. Salehnia M. Autograft of vitrified mouse ovaries using ethylene glycol as cryoprotectant. Exp Anim 2002; 51: 509-512. [DOI:10.1538/expanim.51.509]
9. Sugimoto M, Maeda S, Manabe N, Miyamoto H. Development of infantile rat ovaries autotransplanted after cryopreservation by vitrification. Theriogenology 2000; 53: 1093-1103. [DOI:10.1016/S0093-691X(00)00255-7]
10. Hani T, Tachibe T, Shingai S, Kamada N, Ueda O, Jishage K. Fertility of mice receiving vitrified adult mouse ovaries. Reproduction 2006; 131: 681-687. [DOI:10.1530/rep.1.01030]
11. Hasegawa A, Mochida N, Ogasawara T, Koyama K. Pup birth from mouse oocytes in preantral follicles derived from vitrified and warmed ovaries followed by in vitro growth, in vitro maturation, and in vitro fertilization. Fertil Steril 2006; 86: 1182-1192. [DOI:10.1016/j.fertnstert.2005.12.082]
12. Boonkusol D, Faisaikarm T, Dinnyes A, Kitiyanant Y. Effects of vitrification procedures on subsequent development and ultrastructure of in vitro-matured swamp buffalo (Bubalus bubalis) oocytes. Reprod Fertil Dev 2007; 19: 383-391. [DOI:10.1071/RD06097]
13. Mazoochi T, Salehnia M, Valojerdi MR, Mowla SJ. Morphologic, ultrastructural, and biochemical identification of apoptosis in vitrified-warmed mouse ovarian tissue. Fertil Steril 2008; 90: 1480-1486. [DOI:10.1016/j.fertnstert.2007.07.1384]
14. Dhali A, Manik RS, Das SK, Singla SK, Palta P. Post-vitrification survival and in vitro maturation rate of buffalo (Bubalus bubalis) oocytes: effect of ethylene glycol concentration and exposure time. Anim Reprod Sci 2000; 63:159-165. [DOI:10.1016/S0378-4320(00)00170-6]
15. Kasai M, Komi JH, Takakamo A, Tsudera H, Sakurai T, Machida T. A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J Reprod Fertil 1990; 89: 91-97. [DOI:10.1530/jrf.0.0890091]
16. Rayos AA, Takahashi Y, Hishinuma M, Kanagawa H. Quick freezing of unfertilized mouse oocytes using ethylene glycol with sucrose or trehalose. J Reprod Fertil 1994; 100: 123-129. [DOI:10.1530/jrf.0.1000123]
17. Valojerdi MR, Salehnia M. Developmental potential and ultrastructural injuries of metaphase II (MII) mouse oocytes after slow freezing or vitrification. J Assist Reprod Genet 2005; 22: 119-127. [DOI:10.1007/s10815-005-4876-8]
18. Chen SU, Chien CL, Wu MY. Novel direct cover vitrification for cryopreservation of ovarian tissues increases follicle viability and pregnancy capability in mice. Hum Reprod 2006; 21: 2794-2800. [DOI:10.1093/humrep/del210]
19. Haidari K, Salehnia M, Valojerdi MR. The effect of leukemia inhibitory factor and coculture on the in vitro maturation and ultrastructure of vitrified and nonvitrified isolated mouse preantral follicles. Fertil Steril 2008; 90: 2389-2397. [DOI:10.1016/j.fertnstert.2007.10.052]
20. Nagano M, Atabay EP, Atabay EC, Hishinuma M, Katagiri S, Takahashi Y. Effects of isolation method and pre-treatment with ethylene glycol or raffinose before vitrification on in vitro viability of mouse preantral follicles. Biomed Res 2007; 28: 153-160. [DOI:10.2220/biomedres.28.153]
21. Rahimi G, Isachenko E, Sauer H, Isachenko V, Wartenberg M, Hescheler J, et al. Effect of different vitrification protocols for human ovarian tissue on reactive oxygen species and apoptosis. Reprod Fertil Dev 2003; 15: 343-349. [DOI:10.1071/RD02063]
22. Santos RR, Tharasanit T, Van Haeften T, Figueiredo JR, Silva JR, Van den Hurk R. Vitrification of goat preantral follicles enclosed in ovarian tissue by using conventional and solid-surface vitrification methods. Cell Tissue Res 2007; 327: 167-176. [DOI:10.1007/s00441-006-0240-2]
23. Sakai A, Engelmann F. Vitrification, encapsulation-vitrification and droplet-vitrification: a review. Cryo Letters 2007; 28: 151-172.
24. Fauque P, Ben Amor A, Joanne C, Agnani G, Bresson JL, Roux C. Use of trypan blue staining to assess the quality of ovarian cryopreservation. Fertil Steril 2007; 87: 1200 -1207. [DOI:10.1016/j.fertnstert.2006.08.115]
25. Hyttel P, Vajta G, Callesen H. Vitrification of bovine oocytes with the open pulled straw method: ultrastructural consequences. Mol Reprod Dev 2000;56: 80-88 https://doi.org/10.1002/(SICI)1098-2795(200005)56:1<80::AID-MRD10>3.0.CO;2-U [DOI:10.1002/(SICI)1098-2795(200005)56:13.0.CO;2-U]
26. Kuleshova LL, MacFarlane DR, Trounson AO, Shaw JM. Sugars exert a major influence on the vitrification properties of ethylene glycol-based solutions and have low toxicity to embryos and oocytes. Cryobiology 1999; 38: 119-130. [DOI:10.1006/cryo.1999.2153]
27. Isachenko E, Isachenko V, Rahimi G, Nawroth F. Cryopreservation of human ovarian tissue by direct plunging into liquid nitrogen. Eur J Obstet Gynecol Reprod Biol 2003; 108: 186-193. [DOI:10.1016/S0301-2115(02)00465-7]
28. Matos MH, Andrade ER, Lucci CM, Báo SN, Silva JR, Santos RR, et al. Influences of FSH and EGF on primordial follicles during in vitro culture of caprine ovarian cortical tissue. Theriogenology 2004; 62: 65-80. [DOI:10.1016/j.theriogenology.2003.07.025]
29. Santos RR, Rodrigues AP, Costa SH, Silva JR, Matos MH, Lucci CM, et al. Histological and ultrastructural analysis of cryopreserved sheep preantral follicles. Anim Reprod Sci 2006; 91: 249-263. [DOI:10.1016/j.anireprosci.2005.04.013]
30. Green DR, Reed JC. Mitochondria and apoptosis. Science 1998; 281: 1309-1312. [DOI:10.1126/science.281.5381.1309]
31. Van Blerkom J. Mitochondria as regulatory forces in oocytes, preimplantaion embryos and stem cells. Reprod Biomed Online 2008; 16: 553-569. [DOI:10.1016/S1472-6483(10)60463-4]
32. Celestino JJ, Santos RR, Lopes CA, Martins FS, Matos MH, Melo MA, et al. Preservation of bovine preantral follicle viability and ultra-structure after cooling and freezing of ovarian tissue. Anim Reprod Sci 2008; 108: 309-318 [DOI:10.1016/j.anireprosci.2007.08.016]
33. Hovatta O. Cryopreservation and culture of human primordial and primary ovarian follicles. Reprod Biomed Online 2005; 10: 729-734. [DOI:10.1016/S1472-6483(10)61116-9]
34. Kim SS, Yang HW, Kang HG, Lee HH, Lee HC, Ko DS, et al. Quantitative assessment of ischemic tissue damage in ovarian cortical tissue with or without antioxidant (ascorbic acid) treatment. Fertil Steril 2004; 82: 679-685. [DOI:10.1016/j.fertnstert.2004.05.022]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb