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Reproductive Biomedicine
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Volume 18, Issue 10 (October 2020)
IJRM 2020, 18(10): 825-836 |
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Hajimaghsoudi E, Darbeheshti F, Kalantar S M, Javaheri A, Mirabutalebi S H, Sheikhha M H. Investigating the expressions of miRNA-125b and TP53 in endometriosis. Does it underlie cancer-like features of endometriosis? A case-control study. IJRM 2020; 18 (10) :825-836
URL: http://ijrm.ir/article-1-1471-en.html
URL: http://ijrm.ir/article-1-1471-en.html
Elnaz Hajimaghsoudi1 
, Farzaneh Darbeheshti2 , Seyed Mehdi Kalantar3 , Atiyeh Javaheri4 , Seyed Hamidreza Mirabutalebi5 , Mohammad Hasan Sheikhha * 6
, Farzaneh Darbeheshti2 , Seyed Mehdi Kalantar3 , Atiyeh Javaheri4 , Seyed Hamidreza Mirabutalebi5 , Mohammad Hasan Sheikhha * 6
1- Department of Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Breast Cancer Association (BrCA), Universal Scientific Education and Research Network (USERN), Tehran, Iran.
3- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Department of Obstetrics and Gynecology, Faculty of Medicine, Shahid Sadoughi Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
6- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,sheikhha@yahoo.com
2- Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Breast Cancer Association (BrCA), Universal Scientific Education and Research Network (USERN), Tehran, Iran.
3- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Department of Obstetrics and Gynecology, Faculty of Medicine, Shahid Sadoughi Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
6- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,
Abstract: (1781 Views)
Background: Endometriosis is generally considered as a benign condition; however, there is a possibility for it to become cancerous. miR-125b is upregulated in both endometriotic tissues and serum samples of women with endometriosis but its potential targets in endometriosis are still not fully understood.
Objective: The role of miR-125b in the regulation of TP53 expression in endometriosis was tested with a bioinformatics approach. In addition, the expression of miR-125b and TP53 in both eutopic (Eu-p) and ectopic endometrium (Ec-p) in the endometrium tissues of women with endometriosis was compared to those in the normal endometrium tissues of controls (Normal).
Materials and Methods: In this case-control study, the Eu-p and Ec-p samples were collected from 20 women who underwent laparoscopic surgery, and the normal endometrium tissues were collected from 20 controls with no evidence of endometriosis. For bioinformatics approach, a protein-protein interaction network was constructed based on co-expressed potential targets of miR-125b. Quantitative polymerase chain reaction technique was used for the measurement of miR125b and TP53 expression.
Results: Our results showed that miR-125b was significantly overexpressed in Ec-p (p-value: 0.021). In addition, there was a significant TP53 under expression in both the Ec-p and Eu-p samples compared with the Normal tissues (p-value: 0.003).
Conclusion: The negative correlation between miR-125b and TP53 as well as a noticeable decreased expression of TP53 in both Ec-p and Eu-p samples may be interpreted as the roles of miR-125b/TP53 axis in the pathogenesis of endometriosis. In addition, these findings and bioinformatic analyses imply a possible role of miR-125b in cancer-like features of endometriosis.
Type of Study: Original Article |
Subject:
Reproductive Genetics
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