شنبه 1 دی 1403
[Archive]
دوره 7، شماره 3 - ( 4-1388 )
جلد 7 شماره 3 صفحات 134-129 |
برگشت به فهرست نسخه ها
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Bahmanpour S, Namavar M R, Talaei T, Mazaheri Z, Monabati A. The effects of follicular fluid on human X Y bearing sperm ratio by Fluorescent in Situ Hybridization (FISH). IJRM 2009; 7 (3) :129-134
URL: http://ijrm.ir/article-1-154-fa.html
URL: http://ijrm.ir/article-1-154-fa.html
The effects of follicular fluid on human X Y bearing sperm ratio by Fluorescent in Situ Hybridization (FISH). International Journal of Reproductive BioMedicine. 1388; 7 (3) :129-134
چکیده: (2434 مشاهده)
Background: Some evidences showed that the secretion of uterine tube vagina and follicular fluid (FF) affects X and Y-chromosome populations. Sperm selection with X or Y chromosome can added to oocyte for gender desired. The isolation of X Y sperm have done and all efforts in this field are done to make culture media similar to in vivo condition. The objective of this study was to find if the FF can influence the ratio of X or Y chromosomes therefore we added human FF to culture media to separate X and Y sperms.
Materials and Methods: Normal semen sample from 36 healthy men were selected. Then the samples were divided into control and experimental groups: control group sperms have been incubated with conventional culture media (Ham,s F-10) and experimental group with conventional culture media + 10% human FF. For sperm isolation swim up technique was used. After 24 hours of incubation slides smear were prepared. Then we used the Fluorescent in Situ Hybridization (FISH) method to evaluate the effect of follicular fluid on the population ratio of X and Y containing sperms.
Results: Although the incubation of sperm in FF and Ham,s F-10 increased Y sperm (59.44 % in control and 61.42% in experimental groups) in comparison with X sperm (40.56% in control and 38.5 in experimental groups) significantly (p<0.05), but the Y (or X) bearing sperm did not significantly change in experimental group in comparison with Y (or X) bearing sperm in control group.
Conclusion: This study showed that using the swim up method for collecting sperms and adding FF to culture media can improve some sperm parameters, but did not has significant effects on population of X and Y sperm.
Materials and Methods: Normal semen sample from 36 healthy men were selected. Then the samples were divided into control and experimental groups: control group sperms have been incubated with conventional culture media (Ham,s F-10) and experimental group with conventional culture media + 10% human FF. For sperm isolation swim up technique was used. After 24 hours of incubation slides smear were prepared. Then we used the Fluorescent in Situ Hybridization (FISH) method to evaluate the effect of follicular fluid on the population ratio of X and Y containing sperms.
Results: Although the incubation of sperm in FF and Ham,s F-10 increased Y sperm (59.44 % in control and 61.42% in experimental groups) in comparison with X sperm (40.56% in control and 38.5 in experimental groups) significantly (p<0.05), but the Y (or X) bearing sperm did not significantly change in experimental group in comparison with Y (or X) bearing sperm in control group.
Conclusion: This study showed that using the swim up method for collecting sperms and adding FF to culture media can improve some sperm parameters, but did not has significant effects on population of X and Y sperm.
نوع مطالعه: Original Article |
فهرست منابع
1. Mittwoch U. Sex in mythology and history. Arq Bras Endocrinol Metab 2005; 49:7-13. [DOI:10.1590/S0004-27302005000100003]
2. Scott Sills E. Sex selection of human sperm: Evolution of current techniques and applications. Arch. Gynecol. Obstet 1998; 261: 109-115. [DOI:10.1007/s004040050209]
3. Paasch U, Grunewald S, Glander HJ. Sperm selection in assisted reproductive techniques. Soc Reprod Fertil Suppl 2007; 65: 515-525.
4. Graves JA, Delbridge ML. The X- a sexy chromosome. Bioassays 2001; 23: 1091-1094. [DOI:10.1002/bies.10026]
5. Jain T, Missmer SA, Gupta RS, Hornstein MD, Preimplantation sex selection demand and preferences in an infertility population. Fert Steril 2005; 83: 649-658. [DOI:10.1016/j.fertnstert.2004.11.023]
6. Gledhill BL. Selection and separation of X- and Y- chromosome-bearing mammalian sperm. Gamete Res 1988; 20: 377-395. [DOI:10.1002/mrd.1120200312]
7. Johnson LA, Welch GR, Keyvanfar K, Dorfmann A, Fugger EF, Schulman JD. Gender preselection in humans? Flow cytometric separation of X and Y spermatozoa for the prevention of X-linked diseases. Hum Reprod 1993; 8:1733. [DOI:10.1093/oxfordjournals.humrep.a137925]
8. Levinson G, Keyvanfar K, Wu JC, Fugger EF, Fields RA, Harton GL, et al. DNA-based X-enriched sperm separation as an adjunct to preimplantation genetic testing for the prevention of X-linked disease. Hum Reprod 1995; 10: 979-982. [DOI:10.1093/oxfordjournals.humrep.a136081]
9. Karabinus DS .Flow cytometric sorting of human sperm: MicroSort clinical trial update. Theriogenology 2009; 71: 74-79. [DOI:10.1016/j.theriogenology.2008.09.013]
10. Schulman JD, Karabinus DS. Scientific aspects of preconception gender selection. Reprod Biomed Online. 2005; 10: 125-128. [DOI:10.1016/S1472-6483(10)62217-1]
11. Grant V.J. Sex determination and the maternal dominance hypothesis. Hum Reprod 1996; 11: 2371-2375. [DOI:10.1093/oxfordjournals.humrep.a019117]
12. Seli E, Sakkas D. Sperm nuclear determinants of reproductive outcome: implications for ART. Hum Reprod 2005; 11: 337-349. [DOI:10.1093/humupd/dmi011]
13. Burrello N, Vicari E, D'Amico L, Satta A, D'Agata R, Calogero AE. Human follicular fluid stimulates the acrosomal reaction by interacting with γ -aminobutyric acid receptor. Fertility and sterility 2004; 82: 1086-1090 [DOI:10.1016/j.fertnstert.2004.04.028]
14. Mendoza C, Tesarik J. Effects of follicular fluid on sperm movement characteristics. Fertil Steril 1991; 56: 588-589. [DOI:10.1016/0020-7292(91)90793-5]
15. Schweigert FJ, Steinhagen B, Raila J, Siemann A, Peet D, Buscher U. Concentrations of carotenoids, retinol and alpha-tocopherol in plasma and follicular fluid of women undergoing IVF. Hum Reprod 2003; 18: 1259-1264. [DOI:10.1093/humrep/deg249]
16. Jeon BG, Moon JS, Kim KC, Lee HJ, Choe SY Rho GJ. Follicular fluid enhances sperm attraction and its motility in human. J of Assist Reprod and genet 2000; 18: 407-412. [DOI:10.1023/A:1016674302652]
17. Chen Z. Yan J, Feng HL. Aneuploid analysis of tripronuclear zygotes derived from in vitro fertilization and intracytoplasmic sperm injection in humans. Fert Steril 2005, 6: 1845-1848. [DOI:10.1016/j.fertnstert.2004.11.076]
18. Flaherty SP, Matthews CD. Application of modern molecular techniques to evaluate sperm sex selection methods. Mol Hum Reprod 1996; 2:937-942. [DOI:10.1093/molehr/2.12.937]
19. Ericsson RJ. X, Y sperm. Contracept Fert Sex 1976; 8: 655-668.
20. Cheng FP, Fazeli AR, Voorhout WF, Tremoleda JL, Bevers MM, Colenbrander B. Progesterone in mare follicular fluid induces the acrosome reaction in stallion sperm and enhances in vitro binding to zona pellucida. International journal of andrology 1998; 21: 57-66. [DOI:10.1046/j.1365-2605.1998.00096.x]
21. Kobayashi J, Oguro H, Uchida H, Kohsaka T, Sasada H, Sato E. Assessment of bovine X- and Y-bearing sperm in fractions by discontinuous percoll gradients with rapid fluorescence in situ hybridization. J Reprod Dev 2004; 4: 463-469. [DOI:10.1262/jrd.50.463]
22. Johnson LA, Flook J, Hawk HW. Sex preselection in rabbits: live births from X and Y sperm separated by DNA and cell sorting. Biol Reprod 1989; 41: 199-203. [DOI:10.1095/biolreprod41.2.199]
23. Yan J, Feng HL, Chen ZJ, Hu J, Gao X, Qin Y. Influence of swim-up time on the ratio of X- and Y-bearing spermatozoa. European Journal of Obstetrics & Gynecology and Reproductive Biology 2006; 129: 150-154. [DOI:10.1016/j.ejogrb.2006.02.020]
24. Gawecka-Szczgiel M, Kurpisz M. X, Y chromosome-bearing sperm selection and detection methods. Areview. Folia Histochem cytobiol 1995; 33: 219-127.
25. Van Dyk Q, Mahony M C Hodgen G D. Differential binding of X- and Y-chromosome-bearing human spermatozoa to zona pellucida in vitro. Andrologia 2001; 33: 199-205. [DOI:10.1046/j.1439-0272.2001.00427.x]
26. Flaherty SP, Michalowska J,Swann NJ, Dmowski WP, Mathews CT. Albumin gradient do not enrich Y bearing spermatozoa. Hum Reprod 1997; 12: 938-942. [DOI:10.1093/humrep/12.5.938]
27. Zhou Z, Su L, Tang G. Isolation of X- and Y-bearing spermatozoa. J Chin Eugen Genet 1995; 5: 95-97.
28. Han TL, Flaherty SP, Ford JH, Matthews CD. Detection of X and Y bearing human sperm after motile sperm isolation by swim-up. Fertil Steril 1993; 60: 1046-1051. [DOI:10.1016/S0015-0282(16)56408-5]
| بازنشر اطلاعات | |
 |
این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است. |
