Volume 18, Issue 9 (September 2020)                   IJRM 2020, 18(9): 755-764 | Back to browse issues page

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Mohammadzadeh M, Khalili M A, Ramezani V, Hamishehkar H, Dehghan Marvast L, Mangoli E, et al . Does resveratrol affect prepared sperm parameters and chromatin quality in normozoospermic and asthenozoospermic patients before and after freezing? A lab trial study. IJRM 2020; 18 (9) :755-764
URL: http://ijrm.ir/article-1-1620-en.html
Does resveratrol affect prepared sperm parameters and chromatin quality in normozoospermic and asthenozoospermic patients before and after freezing? A lab trial study
Masoomeh Mohammadzadeh1 , Mohammad Ali Khalili1 , Vahid Ramezani2 , Hamed Hamishehkar3 , Laleh Dehghan Marvast1 , Esmat Mangoli1 , Mahya Rajabi4 , Zhima Akhavan Sales5 , Ali Reza Talebi * 6
1- Department of Reproductive Biology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Department of Pharmaceutics, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5- Department of Immunology, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
6- Department of Reproductive Biology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. , prof_talebi@hotmail.com
Abstract:   (2070 Views)
Background: Previous studies have examined the effect of resveratrol as a potent antioxidant for free radicals in semen. While, the prepared spermatozoa are more affected by ROS factors due to centrifugation and incubation.
Objective: To evaluate the RSV's effects on the prepared sperm parameters and chromatin quality in both normozoospermic and asthenozoospermic cases before and after freezing.
Materials and Methods: The sample of 10 normozoospermic and asthenozoospermic men was prepared through the swim-up method. The groups were then divided into two samples of control and experimental (exposure to 30 µmol/l of RSV) to evaluate and compare the sperm parameters and chromatin quality before and after freezing.
Results: The motility and viability of spermatozoa were seen to be significantly different before and after freezing separately in the control and treatment samples of the groups (p ≤ 0.001 and p = 0.001, respectively). However, the stated difference between the control and treatment samples of normozoospermic and asthenozoospermic patients were not significant (p > 0.05). In addition, the sperm morphology and chromatin quality were not significantly different between the two samples of each group; nonetheless, chromatin quality of the treated sample was better than that of the control before and after freezing.
Conclusion: Despite the protective effects of RSV on the semen samples, RSV cannot affect significantly the prepared sperms parameters and chromatin quality in normozoospermic and asthenozoospermic patients.
Keywords: Resveratrol, Chromatin, Motility, Spermatozoa, Freeze.
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Type of Study: Original Article | Subject: Reproductive Andrology
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